BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,570,887	T→C	5.0%	F264F (TTT→TTC)	yibH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,570,887	0	T	C	5.0%	413.0 / 5.9	138	F264F (TTT→TTC)	yibH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (81/50); new base C (2/5); total (83/55)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

CAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAACGCGCTACCTGGG  >  minE/2570817‑2570956

cAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                                                       >  1:1563921/1‑71 (MQ=255)

 aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc                                                                      <  1:5590133/71‑1 (MQ=255)

 aGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTc                                                                      <  1:447287/71‑1 (MQ=255)

      ccTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCgg                                                                    >  1:5018281/1‑68 (MQ=255)

      ccTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaa                                                                 <  1:4446137/71‑1 (MQ=255)

      ccTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCa                                                                  >  1:364113/1‑70 (MQ=255)

       gctGCCGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCag                                                                 <  1:4087924/68‑2 (MQ=255)

         tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCTAAAACGGCAAATTGTCGCCCAAttt                                                                       >  1:2746410/1‑62 (MQ=255)

         tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                                                       >  1:3171260/1‑62 (MQ=255)

         tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                                                       >  1:1634008/1‑62 (MQ=255)

         tGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaaaa                                                               >  1:4206615/1‑70 (MQ=255)

         tGCCGCTGCGTCCGGTGATGGTCTTCAT‑CCCGAGCAAAAACGGCAAATTGTCGCCCAAttt                                                                       >  1:1560527/1‑61 (MQ=255)

           ccGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTc                                                            <  1:1531971/71‑1 (MQ=255)

           ccGCTACGTCCGGTGATGGTGTTTATACCCGATCAGAAACGACAAATCGTGGCGCAGTTCCGTCAGAACTc                                                            >  1:352963/1‑71 (MQ=255)

             gCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGc                                                          <  1:774436/71‑1 (MQ=255)

              cTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCt                                                         >  1:3564061/1‑71 (MQ=255)

                 cGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCg                                                           <  1:2480910/66‑1 (MQ=255)

                 cGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCg                                                           <  1:4251836/66‑1 (MQ=255)

                 cGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCg                                                           <  1:3251621/66‑1 (MQ=255)

                   tCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTAc                                                    <  1:3943/71‑1 (MQ=255)

                    ccGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTAc                                                    <  1:5338924/70‑1 (MQ=255)

                       gTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCt                                                <  1:2653371/71‑1 (MQ=255)

                        tGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTg                                               <  1:5162461/71‑1 (MQ=255)

                          aTGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGa                                              <  1:2546995/70‑1 (MQ=255)

                            ggTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAc                                           >  1:1499124/1‑71 (MQ=255)

                             gTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAcc                                          <  1:4823359/71‑1 (MQ=255)

                               cTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaaaa                                                               >  1:211175/1‑48 (MQ=255)

                               cTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTg                                        >  1:5380065/1‑71 (MQ=255)

                                ttCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTCCGGCaa                                                                 <  1:733199/45‑1 (MQ=255)

                                  cATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaaaa                                                               >  1:260624/1‑45 (MQ=255)

                                  cATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTAc                                                    <  1:3973375/56‑1 (MQ=255)

                                  cATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGc                                      >  1:4865766/1‑70 (MQ=255)

                                  cATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGc                                      >  1:4302455/1‑70 (MQ=255)

                                   aTCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCg                                     <  1:2946237/70‑1 (MQ=255)

                                    tCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGt                                                  >  1:3639720/1‑56 (MQ=255)

                                    tCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCgat                                   <  1:2224427/71‑1 (MQ=255)

                                    tCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCgat                                   <  1:1789805/71‑1 (MQ=255)

                                    tCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCgat                                   <  1:3762874/71‑1 (MQ=255)

                                     ccccGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCgat                                   >  1:5520658/1‑70 (MQ=255)

                                      cccGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCgatga                                 >  1:4876718/1‑71 (MQ=255)

                                       ccGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaaaa                                                               >  1:3021805/1‑40 (MQ=255)

                                       ccGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaaaa                                                               >  1:3451386/1‑40 (MQ=255)

                                       ccGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaaaa                                                               >  1:662394/1‑40 (MQ=255)

                                        cGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCaa                                                                 <  1:4974498/37‑1 (MQ=255)

                                        cGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGc                                                          <  1:4690872/44‑1 (MQ=255)

                                        cGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGaaa                                            >  1:3267301/1‑58 (MQ=255)

                                        cGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGaaa                                            >  1:3885591/1‑58 (MQ=255)

                                         gAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGc                              <  1:4336821/71‑1 (MQ=255)

                                          aGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTg                                                        >  1:5443221/1‑44 (MQ=255)

                                          aGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCg                             >  1:5579984/1‑71 (MQ=255)

                                          aGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGATGTTACGTCTGAAACCTGGCGATGATGCg                             >  1:635513/1‑71 (MQ=255)

                                           gCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCg                             >  1:401519/1‑70 (MQ=255)

                                            cAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGa                           <  1:3240557/71‑1 (MQ=255)

                                             aaaaaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGatgatg                               <  1:1068369/66‑1 (MQ=255)

                                             aaaaaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCgg                            >  1:540788/1‑69 (MQ=255)

                                              aaaaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGaa                                             <  1:5561314/51‑1 (MQ=255)

                                              aaaaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGc                                      >  1:3295029/1‑58 (MQ=255)

                                              aaaaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAg                         >  1:5067464/1‑71 (MQ=255)

                                               aaaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAgt                        >  1:4059834/1‑71 (MQ=255)

                                                aaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCt                                                         >  1:5013824/1‑37 (MQ=255)

                                                aaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTa                                                     >  1:5233899/1‑41 (MQ=255)

                                                aaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTa                                                     >  1:4972012/1‑41 (MQ=255)

                                                aaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAc                                           >  1:1337661/1‑51 (MQ=255)

                                                aaCGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAc                                           >  1:2205286/1‑51 (MQ=255)

                                                aaCGGC‑AATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAgtgg                      >  1:3392502/1‑71 (MQ=255)

                                                  cGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGt                                                       <  1:2881908/37‑1 (MQ=255)

                                                    gCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCgat                                   <  1:1062244/55‑1 (MQ=255)

                                                    gCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGgtgt                   >  1:3426417/1‑71 (MQ=255)

                                                     cAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGa                           <  1:2272444/62‑1 (MQ=255)

                                                     cAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGtt                  <  1:11705/71‑1 (MQ=255)

                                                      aaaTTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGttt                 <  1:2566728/71‑1 (MQ=255)

                                                      aaaTTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGttt                 <  1:5227551/71‑1 (MQ=255)

                                                       aaTTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGttt                 >  1:4571260/1‑70 (MQ=255)

                                                        aTTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTaa               <  1:642387/71‑1 (MQ=255)

                                                        aTTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTaa               >  1:3444830/1‑71 (MQ=255)

                                                         ttGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAAc                                           >  1:251902/1‑42 (MQ=255)

                                                         ttGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGGGATGATGCGGAAGTGGTGTTTAAc              <  1:1179816/71‑1 (MQ=255)

                                                         ttGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGaa                          >  1:3739740/1‑59 (MQ=255)

                                                         ttGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTaa               >  1:5143009/1‑70 (MQ=255)

                                                         ttGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAAc              >  1:3446785/1‑71 (MQ=255)

                                                         ttGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAAc              >  1:302682/1‑71 (MQ=255)

                                                         ttGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAAc              >  1:1603168/1‑71 (MQ=255)

                                                          tGTCGCCCAATTTCGGCAAAACTCGCTG‑TACGTCTGa                                              >  1:2496995/1‑37 (MQ=38)

                                                            tCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTaa               >  1:943101/1‑67 (MQ=255)

                                                            tCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTaa               >  1:274479/1‑67 (MQ=255)

                                                             cGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAACgcgc          >  1:5481088/1‑71 (MQ=255)

                                                              gCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAACgcgc          <  1:3302010/70‑1 (MQ=255)

                                                              gCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAACGCGCt         <  1:709668/71‑1 (MQ=255)

                                                               cccAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCgat                                   <  1:1332322/44‑1 (MQ=255)

                                                                 cAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGaa                          >  1:1701262/1‑51 (MQ=255)

                                                                   aTTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAACGCGCTACCTg    >  1:2240594/1‑71 (MQ=255)

                                                                   aTTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAACGCGCTACCTg    >  1:1861035/1‑71 (MQ=255)

                                                                    tttCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCgat                                   <  1:4153272/39‑1 (MQ=255)

                                                                    tttCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAACGCGCTACCTgg   >  1:3583334/1‑71 (MQ=255)

                                                                     ttCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCgatga                                 >  1:1873910/1‑40 (MQ=255)

                                                                     ttCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCgatga                                 >  1:2337657/1‑40 (MQ=255)

                                                                      tCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAgtg                       >  1:2630385/1‑49 (MQ=255)

                                                                      tCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTa                >  1:1818158/1‑56 (MQ=255)

                                                                      tCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAACGCGCTACCTggg  >  1:2453107/1‑70 (MQ=255)

                                                                      tCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAACGCGCTACCTggg  >  1:2251757/1‑70 (MQ=255)

CAGCTGCCTTGCCGCTGCGTCCGGTGATGGTCTTCATCCCCGAGCAAAAACGGCAAATTGTCGCCCAATTTCGGCAAAACTCGCTGTTACGTCTGAAACCTGGCGATGATGCGGAAGTGGTGTTTAACGCGCTACCTGGG  >  minE/2570817‑2570956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`