| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,829 | G→A | 19.1% | I348I (ATC→ATT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,829 | 0 | G | A | 19.1% | 273.4 / 48.7 | 120 | I348I (ATC→ATT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (51/46); new base A (12/11); total (63/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
CGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGG > minE/311759‑311898 | cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:4147432/71‑1 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat < 1:5089969/71‑1 (MQ=255) gCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat < 1:1848565/71‑1 (MQ=255) aaTGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGACCACCAGcataatg > 1:5560352/1‑69 (MQ=2) ggTCGGAATCAACGTCGCGAGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCtt > 1:5645169/1‑71 (MQ=255) cGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa > 1:1014996/1‑71 (MQ=255) aaTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa < 1:5031656/71‑1 (MQ=255) aTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAg > 1:2390574/1‑71 (MQ=255) aTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAg > 1:1071591/1‑71 (MQ=255) aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:3908644/70‑1 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:2748303/1‑71 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt > 1:1417996/1‑71 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:1484380/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:3121986/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:3234591/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:4426564/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtttt < 1:950230/71‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:1937636/66‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:3918965/71‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:1121907/71‑1 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:3370189/1‑70 (MQ=255) gcgcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:1197818/1‑70 (MQ=255) cgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCAc < 1:4399059/71‑1 (MQ=255) ggAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACt > 1:3698759/1‑71 (MQ=255) aGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa > 1:3504734/1‑51 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa < 1:4535045/52‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa < 1:5266851/52‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCa < 1:4895700/52‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:4650009/71‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:5126597/71‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:1266517/71‑1 (MQ=255) ttCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTg < 1:1448128/71‑1 (MQ=255) ttCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCg > 1:4140710/1‑48 (MQ=12) ttCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGt > 1:2819597/1‑70 (MQ=18) tCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTg > 1:3099907/1‑70 (MQ=18) cTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGc > 1:2516665/1‑52 (MQ=255) cTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGaa > 1:2686001/1‑71 (MQ=255) caagAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGaa > 1:4058890/4‑68 (MQ=21) caagAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGaa > 1:716852/4‑68 (MQ=21) cAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAAt < 1:3558846/69‑1 (MQ=255) cAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAAt < 1:207952/69‑1 (MQ=255) aGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATaga < 1:836204/71‑1 (MQ=255) aGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATaga < 1:1660673/71‑1 (MQ=255) agAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATaga > 1:224138/2‑70 (MQ=21) agAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATaga > 1:2064462/2‑70 (MQ=21) gAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATagaaa < 1:4424764/71‑3 (MQ=18) aCAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATagaaa > 1:3916900/1‑67 (MQ=18) aCAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCa < 1:2979806/54‑1 (MQ=255) aCAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtt > 1:4570445/1‑71 (MQ=255) aCAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtt > 1:4664282/1‑71 (MQ=255) aTACATCACCAGGAACACCAGCATAATGGCTTCGaa < 1:3142984/36‑2 (MQ=12) aTACATCACCAGGAACACCAGCATAATGGCTTCGaa < 1:2692604/36‑2 (MQ=12) aTACATAACGAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa > 1:1882947/1‑71 (MQ=255) aTACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGaa < 1:1074778/59‑1 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc > 1:4986943/1‑70 (MQ=255) cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa < 1:1791893/71‑1 (MQ=255) cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa < 1:4763334/71‑1 (MQ=255) cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa < 1:2122378/71‑1 (MQ=255) cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa < 1:3941275/71‑1 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa > 1:4590069/1‑67 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTACGTGAATAGAGATTTTCa > 1:4825532/1‑67 (MQ=255) tcaCCAGGAACACCAGCATAATGGCTTCGAACAGCGtttt < 1:3807416/38‑1 (MQ=16) tAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAc < 1:4887055/71‑1 (MQ=255) aaCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATaga > 1:5133547/1‑57 (MQ=255) aaCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATaga > 1:1121544/1‑57 (MQ=255) aaCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATaga > 1:573596/1‑57 (MQ=255) aaCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATaga > 1:3942716/1‑57 (MQ=255) aacAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:4258127/3‑40 (MQ=255) aCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:4075661/1‑40 (MQ=255) aCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:3716009/1‑40 (MQ=255) aCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTaa > 1:2845680/1‑40 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:163417/37‑1 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:4088301/37‑1 (MQ=255) aGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:5323838/71‑1 (MQ=255) ggAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg > 1:2177368/1‑71 (MQ=255) ggAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtg < 1:3748263/71‑1 (MQ=255) ggAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtg > 1:4970918/1‑71 (MQ=255) gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt < 1:1019905/57‑1 (MQ=255) gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt < 1:326925/57‑1 (MQ=255) gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAt > 1:2514016/1‑54 (MQ=255) gAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATag < 1:4688178/50‑1 (MQ=255) gAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATag < 1:4258475/50‑1 (MQ=255) gAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtgg > 1:4428081/1‑71 (MQ=255) gAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtgg > 1:5289143/1‑71 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt < 1:2953439/71‑1 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt > 1:3048319/1‑71 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg > 1:2557573/1‑69 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg > 1:3123258/1‑69 (MQ=255) acacGAGGATGATCGCTTCGACCAGCGTTTTAACCa < 1:4256686/36‑1 (MQ=255) acacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggtg < 1:4196140/71‑1 (MQ=255) acGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGgtgt < 1:1719512/70‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGaa < 1:1091756/42‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa > 1:1715554/1‑54 (MQ=255) gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg > 1:2362560/1‑70 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa > 1:1313830/1‑52 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa < 1:3480806/71‑1 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGAGTGGTGTCGTa > 1:2278972/1‑71 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACg < 1:2902771/54‑1 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATggg > 1:3992616/1‑71 (MQ=255) gatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg < 1:2354159/49‑1 (MQ=255) | CGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGG > minE/311759‑311898 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |