| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,571,394 | G→T | 17.9% | intergenic (+162/‑1617) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,571,394 | 0 | G | T | 17.9% | 397.8 / 50.5 | 150 | intergenic (+162/‑1617) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (54/69); new base T (15/12); total (69/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
Only 100 of 162 total aligned reads displayed.
TTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCA > minE/2571325‑2571462 | ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAgt < 1:5305193/71‑2 (MQ=255)ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:1864496/70‑1 (MQ=255)ttCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:3328060/70‑1 (MQ=255) cATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt > 1:2803109/1‑71 (MQ=255) cATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt > 1:1549294/1‑71 (MQ=255) aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt < 1:3930766/70‑1 (MQ=255) aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTATATCCGTCTTGTTCTGCCGCAGGAt < 1:5026670/70‑1 (MQ=255) tATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt > 1:5231582/1‑68 (MQ=255) tGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc < 1:4784700/71‑1 (MQ=255) ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACta < 1:1616543/71‑1 (MQ=255) ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:3411049/70‑1 (MQ=255) ttAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACt < 1:596909/70‑1 (MQ=255) taataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACta > 1:4223154/1‑70 (MQ=255) ataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata > 1:2060905/1‑70 (MQ=255) aaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga > 1:246516/1‑61 (MQ=255) cTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:3511341/70‑1 (MQ=255) cTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:5097885/70‑1 (MQ=255) tGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:774512/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt > 1:1469927/1‑51 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt > 1:2566562/1‑51 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGAt > 1:1823475/1‑51 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:5436525/67‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:666272/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:4143204/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:4358569/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGACGCAGGATGAGACTATACATCGTCgag < 1:3829152/70‑1 (MQ=255) tGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:3844992/46‑1 (MQ=255) tGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa < 1:3179875/60‑1 (MQ=255) tGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa < 1:1392556/60‑1 (MQ=255) tGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa < 1:4685267/60‑1 (MQ=255) gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:3667859/1‑71 (MQ=255) gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:1537116/1‑71 (MQ=255) aGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:1982580/49‑1 (MQ=255) gtgtGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa < 1:227182/71‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:3201858/40‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:886221/40‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:5117893/40‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:4960781/71‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:4984534/70‑1 (MQ=255) gCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag < 1:4165716/61‑1 (MQ=255) ctctCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:151787/71‑1 (MQ=255) atctcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACg < 1:4986953/63‑1 (MQ=255) atctcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACg < 1:4993226/63‑1 (MQ=255) tctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata > 1:814179/1‑49 (MQ=255) ctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAg < 1:1266480/36‑1 (MQ=255) ctcATTAGATTTCTCATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatga > 1:1943869/1‑71 (MQ=255) tcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgaga > 1:4175455/1‑58 (MQ=255) tcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCacta < 1:4315466/66‑4 (MQ=255) tcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCacta < 1:1135986/66‑4 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata > 1:1435367/1‑46 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata > 1:1706652/1‑46 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:3904393/68‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata > 1:1951030/1‑71 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:552476/68‑1 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTgagaa > 1:1906316/1‑56 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTgagaa > 1:1372611/1‑56 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatgaca < 1:4734283/70‑3 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:3556466/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:2753796/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:1715371/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:1508719/1‑71 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:3939917/37‑1 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa < 1:1107040/60‑1 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:5173566/62‑1 (MQ=255) tGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat > 1:5176006/1‑69 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:5399116/63‑1 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:4112445/1‑62 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAg > 1:2577682/1‑71 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAg > 1:4664338/1‑71 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:1182047/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:947627/70‑1 (MQ=255) gATTTCTTATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGaat > 1:3122013/1‑69 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATg < 1:2994731/66‑1 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATg < 1:231130/66‑1 (MQ=255) tttcttATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCa > 1:4599096/1‑55 (MQ=255) tttcttATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCa > 1:1970997/1‑55 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtcg < 1:1363715/46‑1 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGc < 1:4813418/53‑1 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg > 1:2172702/1‑60 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg > 1:3515198/1‑60 (MQ=255) tttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtc > 1:2823603/1‑71 (MQ=255) tATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcc < 1:2406726/70‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:1538276/55‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcctcc > 1:2529112/1‑71 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg < 1:5372862/69‑1 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG‑AATGATGATATGAGTTGTTCCTCCTGCGACg > 1:1625714/1‑71 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG‑AATGATGATATGAGTTGTTCCTCCTGCGACg > 1:1619006/1‑71 (MQ=255) gTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc < 1:1230706/69‑1 (MQ=255) ttCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:4301107/46‑1 (MQ=255) ttCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:3234548/1‑46 (MQ=255) ttCTGCCCCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:4292406/1‑46 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:4362226/1‑71 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:5671987/1‑71 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGct > 1:3810355/1‑70 (MQ=255) tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:3935302/38‑1 (MQ=255) tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:64923/38‑1 (MQ=255) tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACgg > 1:5083692/1‑67 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:4033043/1‑38 (MQ=255) cGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCg > 1:3029933/1‑60 (MQ=255) aGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc < 1:3721742/59‑1 (MQ=255) | TTCATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCA > minE/2571325‑2571462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |