| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,571,398 | G→C | 18.2% | intergenic (+166/‑1613) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,571,398 | 0 | G | C | 18.2% | 335.1 / 60.8 | 148 | intergenic (+166/‑1613) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (50/71); new base C (15/12); total (65/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01 | |||||||||||
Only 100 of 155 total aligned reads displayed.
ATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAA > minE/2571328‑2571465 | aTTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATg < 1:4985725/71‑1 (MQ=255) tGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGAc < 1:4784700/71‑1 (MQ=255) taataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACta > 1:4223154/1‑70 (MQ=255) ataaGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata < 1:4326404/70‑1 (MQ=255) cTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:5097885/70‑1 (MQ=255) cTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:5004618/70‑1 (MQ=255) cTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:3511341/70‑1 (MQ=255) tGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:3932380/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtc < 1:5436525/67‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgaga < 1:5619635/71‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag < 1:3413843/70‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:4143204/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:2161337/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCga < 1:4358569/69‑1 (MQ=255) gATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGACGCAGGATGAGACTATACATCGTCgag < 1:3829152/70‑1 (MQ=255) tGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa < 1:3179875/60‑1 (MQ=255) tGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACa < 1:4685267/60‑1 (MQ=255) gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:463623/1‑71 (MQ=255) gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:3667859/1‑71 (MQ=255) gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:1537116/1‑71 (MQ=255) gctgtgATCTCATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTgagaa > 1:1551697/3‑67 (MQ=255) aGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:1982580/49‑1 (MQ=255) aGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg < 1:1820505/70‑1 (MQ=255) gtgtGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa < 1:227182/71‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:3810209/71‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:4960781/71‑1 (MQ=255) gCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag < 1:1624458/61‑1 (MQ=255) gCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgag < 1:4165716/61‑1 (MQ=255) ctctCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:151787/71‑1 (MQ=255) atctcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACg < 1:1855778/63‑1 (MQ=255) atctcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACg < 1:4986953/63‑1 (MQ=255) tctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata > 1:814179/1‑49 (MQ=255) ctcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga > 1:2578423/1‑41 (MQ=255) ctcATTAGATTTCTCATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatga > 1:2317238/1‑71 (MQ=255) tcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCgaga > 1:4175455/1‑58 (MQ=255) tcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:4314960/1‑70 (MQ=255) tcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCacta < 1:1135986/66‑4 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata > 1:1706652/1‑46 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACtata > 1:1435367/1‑46 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:1937277/68‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:3904393/68‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata > 1:1951030/1‑71 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata > 1:2811266/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTgagaa > 1:1372611/1‑56 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCActaatg < 1:4527043/67‑1 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatgaca < 1:2542554/70‑3 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:3556466/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:1508719/1‑71 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:3939917/37‑1 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATga < 1:4703125/37‑1 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa < 1:1107040/60‑1 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:5173566/62‑1 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:5399116/63‑1 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:4112445/1‑62 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:947627/70‑1 (MQ=255) gATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgt > 1:383292/1‑71 (MQ=255) gATTTCTTATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGaat > 1:3122013/1‑69 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATg < 1:2994731/66‑1 (MQ=255) tttcttATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCa > 1:5134465/1‑55 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACAtcgtcg < 1:1363715/46‑1 (MQ=255) ttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttgtt > 1:622813/1‑71 (MQ=255) tttATCGGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCaa > 1:731348/1‑53 (MQ=255) tttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtc > 1:2823603/1‑71 (MQ=255) tATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcc < 1:2406726/70‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:3553576/55‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:1538276/55‑1 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTcctcct < 1:5342248/70‑1 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg > 1:3330444/1‑71 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg < 1:5372862/69‑1 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg < 1:3229031/69‑1 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc > 1:1955615/1‑71 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc > 1:1260997/1‑71 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG‑AATGATGATATGAGTTGTTCCTCCTGCGACg > 1:1619006/1‑71 (MQ=255) gTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc < 1:1230706/69‑1 (MQ=255) ttCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:4301107/46‑1 (MQ=255) tCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCaa > 1:2466197/1‑39 (MQ=255) tCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa > 1:813073/1‑38 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:1971366/44‑1 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:5671987/1‑71 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:423132/1‑71 (MQ=255) tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:64923/38‑1 (MQ=255) tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACgg > 1:5083692/1‑67 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:1664118/1‑38 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:4033043/1‑38 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:2298830/1‑38 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctct < 1:5208430/69‑1 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctct < 1:482278/69‑1 (MQ=255) cGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCg > 1:3029933/1‑60 (MQ=255) cAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa < 1:4786608/71‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:4446608/43‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:2142377/43‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:3708620/43‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:5505732/71‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:258067/71‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:3290580/71‑1 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTaa > 1:5210211/1‑63 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa > 1:2546351/1‑69 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:4208551/1‑66 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:711638/1‑66 (MQ=255) tGAGACGATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTaa > 1:1459/1‑63 (MQ=255) | ATTATGTTAATAAGTTCTGGATGAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAA > minE/2571328‑2571465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |