| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,571,418 | T→A | 16.7% | intergenic (+186/‑1593) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,571,418 | 0 | T | A | 16.7% | 339.4 / 54.6 | 138 | intergenic (+186/‑1593) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (62/53); new base A (11/12); total (73/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 139 total aligned reads displayed.
GAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATC > minE/2571350‑2571488 | gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:463623/1‑71 (MQ=255)gAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg > 1:1537116/1‑71 (MQ=255) aGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCg < 1:1820505/70‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatg < 1:4960781/71‑1 (MQ=255) tgCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:4984534/70‑1 (MQ=255) ctctCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgat < 1:151787/71‑1 (MQ=255) atctcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACg < 1:1855778/63‑1 (MQ=255) atctcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACg < 1:4993226/63‑1 (MQ=255) ctcATTAGATTTCTCATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatga > 1:1943869/1‑71 (MQ=255) tcATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:4314960/1‑70 (MQ=255) tcATTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCacta < 1:1135986/66‑4 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg < 1:1937277/68‑1 (MQ=255) cATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGata > 1:2811266/1‑71 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:3581123/68‑1 (MQ=255) aTTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGatat < 1:3135749/71‑1 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCActaatg < 1:4527043/67‑1 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatgaca < 1:2542554/70‑3 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACtaatgaca < 1:4734283/70‑3 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:1715371/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:3556466/1‑71 (MQ=255) aTTGGATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGacat > 1:2753796/1‑71 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCa < 1:1107040/60‑1 (MQ=255) ttGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:5173566/62‑1 (MQ=255) ggATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:4112445/1‑62 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTTTGCATTGTTGAGAACGCACTAATGACATGa < 1:5040939/70‑1 (MQ=255) ggATTTCTTATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGa < 1:947627/70‑1 (MQ=255) gATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgt > 1:383292/1‑71 (MQ=255) gATTTCTTATCCGTCTTGATCTGTCCGATGACCAGACTGTGCATTGTTGAGAACGCACTAATGACATGaat > 1:3122013/1‑69 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATg < 1:231130/66‑1 (MQ=255) ttttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATg < 1:2994731/66‑1 (MQ=255) tttcttATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCa > 1:1970997/1‑55 (MQ=255) tttcttATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCa > 1:5134465/1‑55 (MQ=255) tttcttATCCGTCTTGATCTGCCCGATGACCAGACTGTGCATTGTTGAGAACGCa > 1:4599096/1‑55 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGc < 1:4813418/53‑1 (MQ=255) tttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAatgatg > 1:2172702/1‑60 (MQ=255) ttttATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgttgtt > 1:622813/1‑71 (MQ=255) tttATCGGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCaa > 1:731348/1‑53 (MQ=255) tATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTtcc < 1:2406726/70‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:5384567/55‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:3553576/55‑1 (MQ=255) tCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:1538276/55‑1 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg > 1:3330444/1‑71 (MQ=255) cGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg > 1:5491644/1‑71 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg < 1:3229031/69‑1 (MQ=255) tCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTg < 1:5372862/69‑1 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc > 1:1955615/1‑71 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc > 1:1260997/1‑71 (MQ=255) ttGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCG‑AATGATGATATGAGTTGTTCCTCCTGCGACg > 1:1625714/1‑71 (MQ=255) gTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGAc < 1:1230706/69‑1 (MQ=255) ttCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:4301107/46‑1 (MQ=255) ttCTGCCCCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga > 1:4292406/1‑46 (MQ=255) tCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCaa > 1:2466197/1‑39 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAAtgatga < 1:1971366/44‑1 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:4362226/1‑71 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctc > 1:5671987/1‑71 (MQ=255) cTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGct > 1:3810355/1‑70 (MQ=255) tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:3935302/38‑1 (MQ=255) tGCCGCAGGATGAGACTATACATCGTCGAGATCGCAat < 1:64923/38‑1 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:2298830/1‑38 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:4033043/1‑38 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAatga > 1:1664118/1‑38 (MQ=255) ccGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGctct < 1:5208430/69‑1 (MQ=255) cGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCg > 1:3029933/1‑60 (MQ=255) cAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa < 1:4786608/71‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:3708620/43‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:2142377/43‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTa < 1:5339379/64‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:258067/71‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:5505732/71‑1 (MQ=255) gATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:3290580/71‑1 (MQ=255) aTGAGACTATACATCGTCGAGATCGCAATGATGATATGAgtt < 1:4218532/42‑1 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTaa > 1:5210211/1‑63 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa > 1:2546351/1‑69 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:711638/1‑66 (MQ=255) tGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:4208551/1‑66 (MQ=255) tGAGACGATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTaa > 1:1459/1‑63 (MQ=255) agaCTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAag < 1:3733606/70‑1 (MQ=255) aCTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaaaa > 1:4354855/1‑67 (MQ=255) aCTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAagag > 1:242859/1‑70 (MQ=255) tataCATCGTCGAGATCGCAATGATGTTATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAg > 1:3801726/1‑71 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCTACGGCTCTAATCAAAAAAGAGCAgg < 1:3670887/70‑1 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAAt < 1:5045480/56‑1 (MQ=255) taCATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAg > 1:2680435/1‑69 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:3092649/1‑56 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCa > 1:4450503/1‑56 (MQ=255) cATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGt > 1:4219500/1‑71 (MQ=255) aTCGTCGAGATCGCAATGATGATATGAGTTGTtcct > 1:4080725/1‑36 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:1351916/1‑71 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:224432/1‑71 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:4684665/1‑71 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:4018276/1‑71 (MQ=255) tcgtcgAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGc > 1:3781592/1‑71 (MQ=255) gagaTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAg > 1:5419034/1‑63 (MQ=255) gaTCGCAATGATGATATGAGTTGTTCCTCCTGCGAAGGCTCTaa > 1:1148926/1‑44 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:2593939/49‑1 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAag < 1:4729998/52‑1 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAag < 1:124382/52‑1 (MQ=255) aTCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAgg < 1:84878/58‑1 (MQ=255) aTCGCAATCATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCaaaa < 1:4850145/49‑1 (MQ=255) tCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATc < 1:4509072/71‑1 (MQ=255) | GAGTGTGCTCTCATTGGATTTTTTATCCGTCTTGTTCTGCCGCAGGATGAGACTATACATCGTCGAGATCGCAATGATGATATGAGTTGTTCCTCCTGCGACGGCTCTAATCAAAAAAGAGCAGGAGTGCTTTTCCATC > minE/2571350‑2571488 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |