| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,678 | G→A | 7.6% | intergenic (+1446/‑333) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,678 | 0 | G | A | 7.6% | 305.7 / 10.3 | 105 | intergenic (+1446/‑333) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (47/50); new base A (3/5); total (50/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
GAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCC > minE/2572609‑2572747 | gAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGt > 1:5455027/1‑71 (MQ=255) aaGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTc < 1:1623420/71‑1 (MQ=255) aGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCa < 1:1116168/71‑1 (MQ=255) ccTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTc < 1:5359246/64‑1 (MQ=255) ccTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTc < 1:2057535/64‑1 (MQ=255) aaaGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACt > 1:3448779/1‑71 (MQ=255) aGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGa > 1:1399628/1‑71 (MQ=255) ttATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGAt > 1:1714589/1‑70 (MQ=255) cTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAAc > 1:701554/1‑55 (MQ=255) cTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATa > 1:4163972/1‑60 (MQ=255) tataAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATaaa < 1:3060346/65‑1 (MQ=255) tataAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAAtcc < 1:4310736/70‑3 (MQ=255) tataAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTcc < 1:2750938/71‑1 (MQ=255) tataAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTc < 1:5405058/70‑1 (MQ=255) tataAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTc < 1:3853401/70‑1 (MQ=255) tataAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTc > 1:2850476/1‑70 (MQ=255) ataAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAAtt > 1:5078795/1‑68 (MQ=255) taAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCac > 1:805141/1‑71 (MQ=255) aaCAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATaaaaa > 1:1153860/1‑63 (MQ=255) aaCAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacct < 1:3079356/71‑1 (MQ=255) aaCAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacct < 1:3974861/71‑1 (MQ=255) aGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGa < 1:4321657/48‑1 (MQ=255) ggTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacctacct > 1:3298856/1‑71 (MQ=255) gTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTc < 1:562847/40‑1 (MQ=255) gTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCtt > 1:2416482/1‑71 (MQ=255) tGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:1357980/71‑1 (MQ=255) gCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCa > 1:5379364/1‑60 (MQ=255) gCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc > 1:4964614/1‑69 (MQ=255) gCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc > 1:1142815/1‑69 (MQ=255) gCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa < 1:1422235/71‑1 (MQ=255) cTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCaa < 1:447350/38‑1 (MQ=255) cTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCaa < 1:1561162/38‑1 (MQ=255) tAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAAt < 1:2952006/54‑1 (MQ=255) tAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGc > 1:2094602/1‑71 (MQ=255) tAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGc > 1:49578/1‑71 (MQ=255) aaTCCCAAGGGGATCGATTGCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGcc > 1:2985563/1‑71 (MQ=255) aaTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTc > 1:4097145/1‑55 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGa > 1:2261199/1‑39 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGa > 1:2653905/1‑39 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGa > 1:5448251/1‑39 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTc > 1:890351/1‑54 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc < 1:5210802/71‑1 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGcc < 1:3723592/70‑1 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc > 1:137772/1‑70 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2969901/71‑1 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:5270177/71‑1 (MQ=255) cccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCaa > 1:3999477/1‑71 (MQ=255) cccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCaa > 1:4792942/1‑71 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAAtt < 1:5061806/50‑1 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:1848093/1‑71 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACATACCTTCCAGCCCaa > 1:2296532/1‑70 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:2106058/61‑1 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:1032664/61‑1 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:3296740/61‑1 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc < 1:2416703/71‑1 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc > 1:73009/1‑71 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc > 1:5319246/1‑71 (MQ=255) cAAGGGGATCGATTCCAGAAATAGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:55964/1‑70 (MQ=255) aaGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:1465919/1‑71 (MQ=255) aaGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:4834487/1‑71 (MQ=255) ggggATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCgaa < 1:724763/71‑3 (MQ=255) ggggATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:2091529/1‑69 (MQ=255) ggggATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:3756246/1‑69 (MQ=255) gATCGATTCCAGAAATCGGGTTCAACGGATACTGATaaa < 1:3238734/39‑1 (MQ=255) gATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2036989/62‑1 (MQ=255) gATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGcc > 1:1086747/1‑71 (MQ=255) aTCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCt < 1:423499/71‑1 (MQ=255) gATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGa < 1:1099265/70‑1 (MQ=255) aTTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCaccta < 1:3775956/45‑1 (MQ=255) ttCCAGAAATCGGGTTCAACGGATACTGATAAAAAt < 1:1892832/36‑1 (MQ=255) ttCCAGAAATCGGGTTCAACGGATACTGATAAAAAt < 1:2648276/36‑1 (MQ=255) tCCAGAAATCGGGTTCAACGGATACTGATAAAAATTcc < 1:4185870/38‑1 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa > 1:4658891/1‑49 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa > 1:5590047/1‑49 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa > 1:1602066/1‑49 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:4341378/70‑1 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:1882051/70‑1 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:4871295/71‑1 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:28989/1‑71 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:2017498/71‑1 (MQ=255) gagaTCGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGatat < 1:1170903/67‑1 (MQ=255) gagaTCGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGatat < 1:3519381/67‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2400665/51‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtg < 1:3999812/66‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtata < 1:1731037/71‑1 (MQ=255) agaTCGGATTCAACGGATACTGATAAAAATTCCATCCTCCTTTCAGCCCAATCGGATCCTGAGTGAtata > 1:4143937/3‑70 (MQ=255) aaaTCGGGTTCAACGGATACTGATAAAAATTCCacctacct > 1:300929/1‑41 (MQ=255) aaaTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGccc > 1:5141368/1‑59 (MQ=255) aaaTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTg > 1:2153154/1‑61 (MQ=255) aTCTGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgt < 1:877913/62‑1 (MQ=255) aTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTcc > 1:274741/1‑42 (MQ=255) aTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgt < 1:4897259/62‑1 (MQ=255) aTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCg < 1:1734648/71‑1 (MQ=255) tCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:4050751/47‑1 (MQ=255) tCGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGatc < 1:2424029/55‑3 (MQ=255) ccGGATTCAGCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtga < 1:4494340/62‑2 (MQ=255) ccGGATTCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGc > 1:1660448/2‑71 (MQ=255) ccGGATTCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGc > 1:1231848/2‑71 (MQ=255) cGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAg < 1:171590/42‑1 (MQ=255) ggTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGcccc < 1:2673676/71‑1 (MQ=255) | GAAAGCATACCTAAAGTTATGCTGTATAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCC > minE/2572609‑2572747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |