| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,705 | C→T | 38.3% | intergenic (+1473/‑306) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,705 | 0 | C | T | 38.3% | 139.8 / 165.1 | 167 | intergenic (+1473/‑306) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (58/45); new base T (40/24); total (98/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01 | |||||||||||
Only 100 of 177 total aligned reads displayed.
TAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGT > minE/2572635‑2572775 | aaCAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacct < 1:3974861/71‑1 (MQ=255) aaCAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacc > 1:171268/1‑70 (MQ=255) gTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCtt > 1:2416482/1‑71 (MQ=255) gCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc > 1:4964614/1‑69 (MQ=255) tAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGc > 1:2094602/1‑71 (MQ=255) aaTCCCAAGGGGATCGATTGCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGcc > 1:2985563/1‑71 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGcc < 1:3723592/70‑1 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc > 1:137772/1‑70 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2969901/71‑1 (MQ=255) cccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCaa > 1:4792942/1‑71 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:1848093/1‑71 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:3296740/61‑1 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:1032664/61‑1 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc > 1:5319246/1‑71 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc > 1:73009/1‑71 (MQ=255) cAAGGGGATCGATTCCAGAAATAGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:55964/1‑70 (MQ=255) aaGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:917203/60‑1 (MQ=255) gATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGcc > 1:1086747/1‑71 (MQ=255) aTTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCaccta < 1:3775956/45‑1 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa > 1:1602066/1‑49 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa > 1:5590047/1‑49 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:4871295/71‑1 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:2017498/71‑1 (MQ=255) gagaTCGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGatat < 1:1170903/67‑1 (MQ=255) gagaTCGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGatat < 1:3519381/67‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2400665/51‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtg < 1:3999812/66‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtata < 1:1731037/71‑1 (MQ=255) aaaTCGGGTTCAACGGATACTGATAAAAATTCCacctacct > 1:300929/1‑41 (MQ=255) aaaTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGccc > 1:5141368/1‑59 (MQ=255) aaaTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTg > 1:2153154/1‑61 (MQ=255) aTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCg < 1:1734648/71‑1 (MQ=255) tCGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGatc < 1:2424029/55‑3 (MQ=255) ccGGATTCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGc > 1:1231848/2‑71 (MQ=255) ccGGATTCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGc > 1:1660448/2‑71 (MQ=255) ggTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGcccc < 1:2673676/71‑1 (MQ=255) attCAGCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCgg < 1:5664344/47‑1 (MQ=255) attCAGCGGATACGCATACAGACTCCATCCCCCCTCCAGCCCTATCGGGTCCTGAGTGATATACCGCCCct < 1:2205840/70‑1 (MQ=255) attCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGat > 1:320179/2‑49 (MQ=255) ttCAGCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCTCCCctg > 1:4336450/1‑71 (MQ=255) ttCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAgt < 1:5182467/56‑1 (MQ=255) ttCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGccccag > 1:1136220/1‑69 (MQ=255) ttCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCaa < 1:1049083/43‑1 (MQ=255) ttCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCaa < 1:2808058/43‑1 (MQ=255) ttCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:1139060/60‑1 (MQ=255) tCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCct > 1:4019555/1‑69 (MQ=255) cAACGGATACTGATACATATTCCATCCACCTCTCAACCCAATCGGGTCCGGGGTGATATACCGCCCctgc < 1:5249456/70‑1 (MQ=255) cAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGatc > 1:813282/1‑46 (MQ=255) cAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGccccag > 1:3173499/1‑67 (MQ=255) cAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:4909787/1‑42 (MQ=255) aaaCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt > 1:711242/2‑56 (MQ=255) aaCGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGATATATCGCCCctgcag > 1:1521892/1‑69 (MQ=255) aaCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:2630580/1‑43 (MQ=255) aaCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:3729997/1‑43 (MQ=255) aaCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg > 1:3513883/1‑71 (MQ=255) aCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagcag > 1:3815513/1‑68 (MQ=255) cGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGATATATCGCCCCTGCAGCg < 1:4503678/71‑1 (MQ=255) cGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCg < 1:3995756/71‑1 (MQ=255) cGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg > 1:5666993/1‑71 (MQ=255) ggATACTGATAAAAATTCCATCCCCCTT‑CAGCCCAATCGGATCCTGAGTGATATACCTCCCCTGCAGCggg > 1:2878568/1‑70 (MQ=255) ggATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCgg > 1:5171066/1‑71 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:241324/52‑1 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:1911403/52‑1 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:666565/1‑57 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:1156018/1‑57 (MQ=255) gATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:5212955/1‑70 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc > 1:428937/1‑38 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc > 1:4998431/1‑38 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc > 1:4633215/1‑38 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc > 1:4480579/1‑38 (MQ=255) aTACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGa > 1:3232991/1‑40 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:2729850/1‑70 (MQ=255) tACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAg > 1:2754358/1‑46 (MQ=255) tACTGATAAAAATTCCACCTACCTTCCAGCCCAAt < 1:5058875/35‑1 (MQ=255) tACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGa < 1:1103184/39‑1 (MQ=255) cTGATAAAAATTCCATCCCCCCTTCAGCCCAATCg < 1:2042505/35‑1 (MQ=16) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGa > 1:1606250/1‑43 (MQ=255) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:76943/1‑49 (MQ=255) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGccc > 1:4294827/1‑57 (MQ=255) tGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt < 1:2519766/40‑1 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCt < 1:2534791/40‑1 (MQ=255) tGATAAAAATTCCACCTACCTCCCAGCCCAATCGGAGCCt < 1:432037/40‑1 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:4979797/1‑64 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:1934447/1‑64 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt < 1:5332975/39‑1 (MQ=16) gATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt < 1:1263718/39‑1 (MQ=16) aTAAAAATTCCATCCCGCTTTCAGCCCAATCGGATCCTGAGTGAtatat > 1:3379299/1‑48 (MQ=255) aTAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGAta > 1:2750914/1‑46 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:4383217/1‑49 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:1445588/71‑1 (MQ=255) tAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCagcagc < 1:1807372/60‑1 (MQ=255) tAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:2339800/60‑1 (MQ=255) aaaaaTTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGatat > 1:4746828/1‑45 (MQ=255) aaaaTTCCATCACCCCTTCAGCCCAATCGGATCCTGAGTGAtata > 1:5468939/1‑45 (MQ=255) aaaaTTCCACCTACCTTCCAGCCCAATCGGAGCATGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAcc > 1:4425874/1‑71 (MQ=255) aaTTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATAcctc < 1:4132582/47‑3 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa > 1:4211728/1‑70 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:967637/70‑1 (MQ=255) acctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:309814/50‑1 (MQ=255) cctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt < 1:2116581/71‑1 (MQ=255) | TAAACAGGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGT > minE/2572635‑2572775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |