| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,711 | T→C | 25.9% | intergenic (+1479/‑300) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,711 | 0 | T | C | 25.9% | 270.7 / 105.3 | 166 | intergenic (+1479/‑300) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (70/53); new base C (27/16); total (97/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
Only 100 of 188 total aligned reads displayed.
GGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAG > minE/2572641‑2572780 | ggTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCacctacct > 1:3298856/1‑71 (MQ=255) tGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:1357980/71‑1 (MQ=255) gCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc > 1:1142815/1‑69 (MQ=255) gCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc > 1:4964614/1‑69 (MQ=255) gCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa < 1:1422235/71‑1 (MQ=255) tAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGc > 1:2094602/1‑71 (MQ=255) aTCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGccc < 1:5210802/71‑1 (MQ=255) tCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2969901/71‑1 (MQ=255) ccAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:1848093/1‑71 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:1032664/61‑1 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:3296740/61‑1 (MQ=255) cAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc < 1:2416703/71‑1 (MQ=255) aaGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTc < 1:917203/60‑1 (MQ=255) aaGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:1465919/1‑71 (MQ=255) aaGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:4834487/1‑71 (MQ=255) ggggATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCgaa < 1:724763/71‑3 (MQ=255) ggggATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:3756246/1‑69 (MQ=255) gATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGcc > 1:1086747/1‑71 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa > 1:1602066/1‑49 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCa > 1:4658891/1‑49 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:2017498/71‑1 (MQ=255) gagaTCGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGatat < 1:3519381/67‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCa < 1:2400665/51‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtg < 1:3999812/66‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtata < 1:1731037/71‑1 (MQ=255) aaaTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTg > 1:2153154/1‑61 (MQ=255) aTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgt < 1:4897259/62‑1 (MQ=255) aTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCg < 1:1734648/71‑1 (MQ=255) ccGGATTCAGCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtga < 1:4494340/62‑2 (MQ=255) ggTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGcccc < 1:2673676/71‑1 (MQ=255) attCAGCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCgg < 1:5664344/47‑1 (MQ=255) attCAGCGGATACGCATACAGACTCCATCCCCCCTCCAGCCCTATCGGGTCCTGAGTGATATACCGCCCct < 1:2205840/70‑1 (MQ=255) attCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGat > 1:320179/2‑49 (MQ=255) ttCAGCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCTCCCctg > 1:4336450/1‑71 (MQ=255) ttCAACGTATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:2577749/1‑60 (MQ=255) ttCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGccccag > 1:1136220/1‑69 (MQ=255) ttCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCaa < 1:1049083/43‑1 (MQ=255) ttCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCaa < 1:2808058/43‑1 (MQ=255) ttCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:2413257/1‑60 (MQ=255) tCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCctg > 1:27694/1‑70 (MQ=255) tCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCct > 1:4019555/1‑69 (MQ=255) cAACGGATACTGATACATATTCCATCCACCTCTCAACCCAATCGGGTCCGGGGTGATATACCGCCCctgc < 1:5249456/70‑1 (MQ=255) cAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAAt > 1:4909787/1‑42 (MQ=255) cAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgct > 1:3994771/1‑71 (MQ=255) aaCGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTg > 1:3002682/1‑50 (MQ=255) aaCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAAt > 1:4998908/1‑41 (MQ=255) aaCGGATACTGATAAAAATTCCACCTACCTTCCAg > 1:1215970/1‑35 (MQ=255) aaCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCg > 1:671114/1‑43 (MQ=255) aCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagcag > 1:3815513/1‑68 (MQ=255) aCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagcag > 1:554642/1‑68 (MQ=255) aCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:610003/1‑59 (MQ=255) cGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCg < 1:3995756/71‑1 (MQ=255) ggATACTGATAAAAATTCCATCCCCCTT‑CAGCCCAATCGGATCCTGAGTGATATACCTCCCCTGCAGCggg > 1:2878568/1‑70 (MQ=255) ggATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCgg > 1:5171066/1‑71 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:1911403/52‑1 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:3432235/1‑57 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:5633958/1‑57 (MQ=255) gATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:5212955/1‑70 (MQ=255) gATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtgat > 1:1654182/1‑50 (MQ=255) gATACTGATAAAAATTCCACCTACCTTCCAGCCCAATc > 1:428937/1‑38 (MQ=255) aTACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGa > 1:3232991/1‑40 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:2729850/1‑70 (MQ=255) tACTGATAAAAATTCCATCCCCACTTCAGCCCAATCGGATCCTGAg > 1:4576623/1‑46 (MQ=255) tACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGa < 1:1103184/39‑1 (MQ=255) cTGATAAAAATTCCATCCCCCCTTCAGCCCAATCg < 1:2042505/35‑1 (MQ=16) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:76943/1‑49 (MQ=255) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGccc > 1:4294827/1‑57 (MQ=255) tGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt < 1:3630668/40‑1 (MQ=255) tGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt < 1:2519766/40‑1 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:4979797/1‑64 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt < 1:1263718/39‑1 (MQ=16) gATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt < 1:5332975/39‑1 (MQ=16) aTAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGAtatat > 1:5594015/1‑48 (MQ=255) aTAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGAtata > 1:3729263/1‑48 (MQ=255) aTAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGa > 1:4320881/1‑40 (MQ=255) aTAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGAta > 1:2750914/1‑46 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:4383217/1‑49 (MQ=255) tAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCagcagc < 1:1807372/60‑1 (MQ=255) tAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:2339800/60‑1 (MQ=255) aaaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAc > 1:1167399/1‑71 (MQ=255) aaaaTTCCATCACCCCTTCAGCCCAATCGGATCCTGAGTGAtata > 1:5468939/1‑45 (MQ=255) aaaaTTCCACCTACCTTCCAGCCCAATCGGAGCATGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAcc > 1:4425874/1‑71 (MQ=255) aaTTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGAta > 1:1699164/1‑41 (MQ=255) aaTTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATAcctc < 1:4132582/47‑3 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGat > 1:3171790/1‑70 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa < 1:5065269/70‑1 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa > 1:4211728/1‑70 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:967637/70‑1 (MQ=255) cctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt < 1:2116581/71‑1 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCgg > 1:2143794/1‑50 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt > 1:2631853/1‑70 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgc < 1:158107/44‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgc < 1:5145287/44‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGAGTTATACCGCCCCAGCTGCGGTTAAAGTTACCGAAAATAGAta > 1:2432791/1‑71 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:2439551/1‑70 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTTCGGTTAATGTTACCGATAATAGTta < 1:3063471/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:4763221/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:5310379/69‑1 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg > 1:1943673/1‑71 (MQ=255) ttCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTa > 1:815782/1‑54 (MQ=255) | GGTGGCTAATCCCAAGGGGATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAG > minE/2572641‑2572780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |