| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,727 | G→T | 43.9% | intergenic (+1495/‑284) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,727 | 0 | G | T | 43.9% | 131.1 / 160.9 | 157 | intergenic (+1495/‑284) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (45/43); new base T (44/25); total (89/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 157 total aligned reads displayed.
GATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTC > minE/2572659‑2572794 | gATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGcc > 1:1086747/1‑71 (MQ=255) gATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGa < 1:1099265/70‑1 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:1882051/70‑1 (MQ=255) ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:4341378/70‑1 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:28989/1‑71 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:2017498/71‑1 (MQ=255) gagaTCGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGatat < 1:3519381/67‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtata < 1:1731037/71‑1 (MQ=255) aaaTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTg > 1:2153154/1‑61 (MQ=255) aTCTGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgt < 1:877913/62‑1 (MQ=255) aTCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgt < 1:4897259/62‑1 (MQ=255) ccGGATTCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGc > 1:1231848/2‑71 (MQ=255) ccGGATTCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGc > 1:1660448/2‑71 (MQ=255) ggTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGcccc < 1:2673676/71‑1 (MQ=255) attCAGCGGATACGCATACAGACTCCATCCCCCCTCCAGCCCTATCGGGTCCTGAGTGATATACCGCCCct < 1:2205840/70‑1 (MQ=255) attCAACGGATACTGATACATATTCCATCCACCTCTCAACCCAATCGGGTCCGGGGTGATATACCGccc > 1:3231085/2‑69 (MQ=255) ttCAACGTATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:2577749/1‑60 (MQ=255) ttCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAgt < 1:5182467/56‑1 (MQ=255) ttCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGccccag > 1:1136220/1‑69 (MQ=255) ttCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:4411233/60‑1 (MQ=255) ttCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:2413257/1‑60 (MQ=255) tCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCct > 1:4019555/1‑69 (MQ=255) cAACGGATACTGATACATATTCCATCCACCTCTCAACCCAATCGGGTCCGGGGTGATATACCGCCCctgc < 1:5249456/70‑1 (MQ=255) cAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGccccag > 1:3173499/1‑67 (MQ=255) cAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgct > 1:3994771/1‑71 (MQ=255) aaaCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt > 1:711242/2‑56 (MQ=255) aaCGGATACTGATACATATTCCATCCACCTCTCAACCCAATCGGGTCCGGGGTGATATACCGCCCctgcaa > 1:5397850/1‑69 (MQ=255) aaCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg > 1:3513883/1‑71 (MQ=255) aCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagcag > 1:554642/1‑68 (MQ=255) aCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagcag > 1:3815513/1‑68 (MQ=255) aCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:610003/1‑59 (MQ=255) cGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGATATATCGCCCCTGCAGCg < 1:4503678/71‑1 (MQ=255) cGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCg < 1:3995756/71‑1 (MQ=255) cGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:1588314/53‑1 (MQ=255) ggATACTGATAAAAATTCCATCCCCCTT‑CAGCCCAATCGGATCCTGAGTGATATACCTCCCCTGCAGCggg > 1:2878568/1‑70 (MQ=255) ggATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtga < 1:5222836/52‑2 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:1156018/1‑57 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:5633958/1‑57 (MQ=255) gATACTGATACATATTCCATCCACCTCTCAACCCAATCGGGTCCGGGGTGATATACCGccc < 1:175360/61‑1 (MQ=255) gATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtgat > 1:1654182/1‑50 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:2729850/1‑70 (MQ=255) tACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAg > 1:2754358/1‑46 (MQ=255) tACTGATAAAAATTCCATCCCCACTTCAGCCCAATCGGATCCTGAg > 1:4576623/1‑46 (MQ=255) aCTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGa > 1:240013/1‑44 (MQ=255) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGccc > 1:4294827/1‑57 (MQ=255) tGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt < 1:3630668/40‑1 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCt < 1:2534791/40‑1 (MQ=255) tGATAAAAATTCCACCTACCTCCCAGCCCAATCGGAGCCt < 1:432037/40‑1 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:1934447/1‑64 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:4979797/1‑64 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCt < 1:5332975/39‑1 (MQ=16) aTAAAAATTCCATCCCGCTTTCAGCCCAATCGGATCCTGAGTGAtatat > 1:3379299/1‑48 (MQ=255) aTAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGAtatat > 1:2503737/1‑48 (MQ=255) aTAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGAtata > 1:3729263/1‑48 (MQ=255) aTAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGa > 1:4320881/1‑40 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:1445588/71‑1 (MQ=255) tAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCagcagc < 1:1807372/60‑1 (MQ=255) aaaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAc > 1:1167399/1‑71 (MQ=255) aaaaTTCCACCTACCTTCCAGCCCAATCGGAGCATGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAcc > 1:4425874/1‑71 (MQ=255) aaTTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGAta > 1:1699164/1‑41 (MQ=255) aaTTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATAcctc < 1:4132582/47‑3 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa > 1:4211728/1‑70 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:967637/70‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:2640986/70‑1 (MQ=255) acctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:514564/50‑1 (MQ=255) acctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:309814/50‑1 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:399991/48‑1 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCgg > 1:2143794/1‑50 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg > 1:2150441/1‑49 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgc < 1:5145287/44‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgc < 1:3548074/44‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgc < 1:158107/44‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGAGTTATACCGCCCCAGCTGCGGTTAAAGTTACCGAAAATAGAta > 1:2432791/1‑71 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTTCGGTTAATGTTACCGATAATAGTta < 1:3063471/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGa < 1:3796863/60‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:4763221/69‑1 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg < 1:5513053/71‑1 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg > 1:1943673/1‑71 (MQ=255) ttCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTa > 1:815782/1‑54 (MQ=255) tcAGCCCAATCGGATCCTGAGTGATATACCGcccc < 1:1132602/34‑1 (MQ=37) tcAGCCCAATCGGATCCTGAGTGATATACCGcccc < 1:4242852/34‑1 (MQ=37) tcAGCCCAATCGGATCCTGAGTGATATACCGcccc < 1:3332834/34‑1 (MQ=37) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgc > 1:274979/1‑38 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTTCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:5113808/69‑1 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa < 1:5045414/70‑1 (MQ=255) cAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCgg > 1:701797/1‑42 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:1811317/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc < 1:2124118/70‑1 (MQ=255) ccccATCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc > 1:2273757/1‑71 (MQ=255) cccAATCGGGTCCGGGGTGATATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGcc > 1:64164/1‑71 (MQ=255) cccaTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGccc > 1:1898110/4‑71 (MQ=255) cccaTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGccc > 1:1181916/4‑71 (MQ=255) ccAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGccc > 1:2761371/1‑71 (MQ=255) cAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGata > 1:3425088/1‑52 (MQ=255) cAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:28268/71‑1 (MQ=255) caTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTg < 1:2543541/70‑1 (MQ=255) aTCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt > 1:2463427/1‑43 (MQ=255) tCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAt > 1:4835131/1‑71 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataata > 1:4172927/1‑50 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:5074099/1‑70 (MQ=255) | GATCGATTCCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTC > minE/2572659‑2572794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |