| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,735 | G→A | 7.8% | intergenic (+1503/‑276) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,735 | 0 | G | A | 7.8% | 408.5 / 16.7 | 141 | intergenic (+1503/‑276) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (77/53); new base A (6/5); total (83/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
CCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAT > minE/2572667‑2572802 | ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:1882051/70‑1 (MQ=255)ccAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:4341378/70‑1 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:2017498/71‑1 (MQ=255) cAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:28989/1‑71 (MQ=255) gagaTCGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGatat < 1:3519381/67‑1 (MQ=255) gagaTCGGATTCAACGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGatat < 1:1170903/67‑1 (MQ=255) gAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTtata < 1:1731037/71‑1 (MQ=255) agaTCGGATTCAACGGATACTGATAAAAATTCCATCCTCCTTTCAGCCCAATCGGATCCTGAGTGAtata > 1:4143937/3‑70 (MQ=255) ccGGATTCAGCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtga < 1:4494340/62‑2 (MQ=255) ccGGATTCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGc > 1:1231848/2‑71 (MQ=255) ccGGATTCAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGc > 1:1660448/2‑71 (MQ=255) ggTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGcccc < 1:2673676/71‑1 (MQ=255) attCAGCGGATACGCATACAGACTCCATCCCCCCTCCAGCCCTATCGGGTCCTGAGTGATATACCGCCCct < 1:2205840/70‑1 (MQ=255) ttCAGCGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCTCCCctg > 1:4336450/1‑71 (MQ=255) ttCAACGTATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:2577749/1‑60 (MQ=255) ttCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta < 1:1139060/60‑1 (MQ=255) cAACGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGccccag > 1:3173499/1‑67 (MQ=255) cAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTta > 1:1947126/1‑58 (MQ=255) aaaCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt > 1:711242/2‑56 (MQ=255) aaCGGATACTGATAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGATATATCGCCCctgcag > 1:1521892/1‑69 (MQ=255) aaCGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgctg > 1:3513883/1‑71 (MQ=255) aCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagcag > 1:3815513/1‑68 (MQ=255) aCGGATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagcag > 1:554642/1‑68 (MQ=255) cGGATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCg < 1:3995756/71‑1 (MQ=255) cGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:1588314/53‑1 (MQ=255) cGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg > 1:5666993/1‑71 (MQ=255) ggATACTGATAAAAATTCCATCCCCCTT‑CAGCCCAATCGGATCCTGAGTGATATACCTCCCCTGCAGCggg > 1:2878568/1‑70 (MQ=255) ggATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAgtga < 1:5222836/52‑2 (MQ=255) ggATACTGATAAAAATTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCgg > 1:5171066/1‑71 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:1911403/52‑1 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAgtgt < 1:241324/52‑1 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:3432235/1‑57 (MQ=255) ggATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:1156018/1‑57 (MQ=255) gATACTGATACATATTCCATCCACCTCTCAACCCAATCGGGTCCGGGGTGATATACCGccc < 1:175360/61‑1 (MQ=255) gATACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:5212955/1‑70 (MQ=255) aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:2729850/1‑70 (MQ=255) tACTGATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCcagcag < 1:329436/65‑3 (MQ=255) cTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGccc > 1:4294827/1‑57 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt < 1:4968894/67‑1 (MQ=255) gATAAAAATTCCATCCCCCCTTCAGTCCAATCGGATCCTGAGTGATATACCGCCCCAGCAGCgg > 1:1934447/1‑64 (MQ=255) aTAAAAATTCCATCCCGCTTTCAGCCCAATCGGATCCTGAGTGAtatat > 1:3379299/1‑48 (MQ=255) aTAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGAtatat > 1:5594015/1‑48 (MQ=255) aTAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGAtatat > 1:2503737/1‑48 (MQ=255) aTAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGAtatat > 1:1130632/1‑48 (MQ=255) aTAAAAATTCCATCCCCCTTTCAGCCCAATCGGATCCTGAGTGAtata > 1:3729263/1‑48 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATAc > 1:4383217/1‑49 (MQ=255) aaaaaTTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGatat > 1:4746828/1‑45 (MQ=255) aaaaTTCCATCACCCCTTCAGCCCAATCGGATCCTGAGTGAtata > 1:5468939/1‑45 (MQ=255) aaaaTTCCACCTACCTTCCAGCCCAATCGGAGCATGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAcc > 1:4425874/1‑71 (MQ=255) aaTTCCATCCCCCCTTCAGCCCAATCGGATCCTGAGTGAta > 1:1699164/1‑41 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa < 1:5065269/70‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:967637/70‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:2640986/70‑1 (MQ=255) acctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:309814/50‑1 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCtgctgc < 1:399991/48‑1 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCg > 1:2150441/1‑49 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgc < 1:3548074/44‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgc < 1:158107/44‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgc < 1:5145287/44‑1 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGAGTTATACCGCCCCAGCTGCGGTTAAAGTTACCGAAAATAGAta > 1:2432791/1‑71 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:2439551/1‑70 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:4763221/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:5310379/69‑1 (MQ=255) ttCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTa > 1:815782/1‑54 (MQ=255) tcAGCCCAATCGGATCCTGAGTGATATACCGcccc < 1:1132602/34‑1 (MQ=37) tcAGCCCAATCGGATCCTGAGTGATATACCGcccc < 1:4242852/34‑1 (MQ=37) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCctgc > 1:274979/1‑38 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTTCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:5113808/69‑1 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa < 1:5045414/70‑1 (MQ=255) cAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCgg > 1:701797/1‑42 (MQ=255) cAGCCCAATCGGATCCTGAGTGATATACCGCCCCTGCAGCgg > 1:3910823/1‑42 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:4094487/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:1811317/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:2144679/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc < 1:2124118/70‑1 (MQ=255) ccccATCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc < 1:1764035/71‑1 (MQ=255) ccccATCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc > 1:2273757/1‑71 (MQ=255) cccAATCGGGTCCGGGGTGATATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGcc > 1:64164/1‑71 (MQ=255) cccaTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGccc > 1:1181916/4‑71 (MQ=255) ccAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGccc > 1:2761371/1‑71 (MQ=255) cAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:28268/71‑1 (MQ=255) caTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTg < 1:2543541/70‑1 (MQ=255) caTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTg < 1:3753246/70‑1 (MQ=255) aaTCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTaa < 1:4299080/40‑1 (MQ=255) aTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGa > 1:3363095/1‑71 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataata > 1:4172927/1‑50 (MQ=255) tccTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc < 1:5675327/70‑1 (MQ=255) tccTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc < 1:2827269/70‑1 (MQ=255) ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatca > 1:1458927/1‑71 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc > 1:3606836/1‑54 (MQ=255) tGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat > 1:4848809/1‑70 (MQ=255) gggtgaTATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACtcctcatca < 1:3320751/69‑1 (MQ=255) gAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg < 1:5317765/44‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg < 1:375271/43‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:4024081/70‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTcatcatcat < 1:9140/71‑1 (MQ=255) gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:1850727/1‑61 (MQ=255) gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:3075723/1‑61 (MQ=255) gtgaTATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACtcctcat < 1:4888930/67‑1 (MQ=255) gtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:3995348/65‑1 (MQ=255) | CCAGAAATCGGGTTCAACGGATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAT > minE/2572667‑2572802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |