| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,756 | T→A | 20.5% | intergenic (+1524/‑255) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,756 | 0 | T | A | 20.5% | 249.5 / 60.8 | 117 | intergenic (+1524/‑255) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (41/52); new base A (14/10); total (55/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
ATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATG > minE/2572687‑2572825 | aTACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGt > 1:2729850/1‑70 (MQ=255) tGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGtt < 1:4968894/67‑1 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATg > 1:943676/1‑69 (MQ=255) aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:1445588/71‑1 (MQ=255) aaaaaTTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAc > 1:1167399/1‑71 (MQ=255) aaaaTTCCACCTACCTTCCAGCCCAATCGGAGCATGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAcc > 1:4425874/1‑71 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGat > 1:3171790/1‑70 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa < 1:5065269/70‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:2640986/70‑1 (MQ=255) cctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt < 1:2116581/71‑1 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:2439551/1‑70 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTTCGGTTAATGTTACCGATAATAGTta < 1:3063471/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGa < 1:3796863/60‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:5310379/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:4763221/69‑1 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg < 1:5513053/71‑1 (MQ=255) tCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg > 1:1695969/1‑69 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTTCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:5113808/69‑1 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa < 1:5045414/70‑1 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:1811317/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:4094487/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:2144679/1‑69 (MQ=255) ccccATCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc < 1:1764035/71‑1 (MQ=255) ccccATCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc > 1:2273757/1‑71 (MQ=255) cccAATCGGGTCCGGGGTGATATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGcc > 1:64164/1‑71 (MQ=255) cccaTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGccc > 1:1898110/4‑71 (MQ=255) ccAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGccc > 1:2761371/1‑71 (MQ=255) cAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGata > 1:3425088/1‑52 (MQ=255) cAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:28268/71‑1 (MQ=255) caTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTg < 1:2543541/70‑1 (MQ=255) caTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTg < 1:3753246/70‑1 (MQ=255) aaTCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTaa < 1:4299080/40‑1 (MQ=255) aTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGa > 1:3363095/1‑71 (MQ=255) aTCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt > 1:2463427/1‑43 (MQ=255) tCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAt > 1:4835131/1‑71 (MQ=255) tCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAt > 1:2551937/1‑71 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataata > 1:4172927/1‑50 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:5074099/1‑70 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtc < 1:3677966/71‑1 (MQ=255) tccTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc < 1:2827269/70‑1 (MQ=255) tccTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc < 1:5675327/70‑1 (MQ=255) ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:5209863/54‑1 (MQ=255) ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatca > 1:1458927/1‑71 (MQ=255) cTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:1215386/71‑1 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:1550200/52‑1 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc > 1:3606836/1‑54 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat > 1:4255053/1‑70 (MQ=255) tGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat > 1:4848809/1‑70 (MQ=255) gggtgaTATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACtcctcatca < 1:3320751/69‑1 (MQ=255) gAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg < 1:5317765/44‑1 (MQ=255) ggtgaTATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACTcctcatcat < 1:3500579/70‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg < 1:375271/43‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:4024081/70‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca > 1:4364238/1‑70 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTcatcatcat < 1:9140/71‑1 (MQ=255) gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:4311450/1‑61 (MQ=255) gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:1850727/1‑61 (MQ=255) gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:3075723/1‑61 (MQ=255) gtgaTATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACtcctcat < 1:4888930/67‑1 (MQ=255) taatataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat < 1:1756528/63‑1 (MQ=255) gtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:3750460/65‑1 (MQ=255) gtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:3995348/65‑1 (MQ=255) aatataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCAtgct > 1:3625482/3‑71 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:459614/54‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:4608315/54‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:5120786/54‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact < 1:704595/70‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact < 1:1963142/70‑1 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGcccc > 1:1408611/2‑53 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACTCCTCATCAtgctg < 1:1260426/70‑2 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc > 1:2377207/2‑62 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTtata > 1:1599657/1‑42 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTtata > 1:3483156/1‑42 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTtat > 1:4843679/1‑41 (MQ=255) taCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc > 1:3598182/1‑49 (MQ=255) taCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCATGctgctg > 1:1084867/1‑71 (MQ=255) aCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:3216827/60‑1 (MQ=255) ccccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCg > 1:1675088/1‑71 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGa > 1:4672802/1‑46 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGa > 1:707945/1‑46 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact < 1:4925133/60‑1 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATactactact < 1:2410494/66‑1 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCgg > 1:1158939/1‑70 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:3793395/55‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:1131489/55‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:878015/55‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:2079703/55‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCa < 1:3059692/71‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:105533/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:3481780/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:4509804/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAg > 1:3132992/1‑71 (MQ=255) gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:946158/51‑1 (MQ=255) gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:1960161/51‑1 (MQ=255) gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGc > 1:3048195/1‑68 (MQ=255) ctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTc > 1:67307/1‑71 (MQ=255) cGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtg < 1:4764864/70‑1 (MQ=255) ttAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtgat < 1:5478436/69‑1 (MQ=255) ttAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtgat < 1:2148182/69‑1 (MQ=255) ttAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtgat < 1:3604419/69‑1 (MQ=255) | ATACTGATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATG > minE/2572687‑2572825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |