| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,761 | G→A | 19.2% | intergenic (+1529/‑250) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,761 | 0 | G | A | 19.2% | 265.7 / 51.1 | 125 | intergenic (+1529/‑250) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (46/55); new base A (14/10); total (60/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
ATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAA > minE/2572693‑2572827 | aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATg > 1:943676/1‑69 (MQ=255)aTAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGtt < 1:1445588/71‑1 (MQ=255) aaaaTTCCACCTACCTTCCAGCCCAATCGGAGCATGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTAcc > 1:4425874/1‑71 (MQ=255) ttCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGat > 1:3171790/1‑70 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa > 1:4211728/1‑70 (MQ=255) ccACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataa < 1:5065269/70‑1 (MQ=255) cACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataat < 1:2640986/70‑1 (MQ=255) ctacctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGt > 1:2631853/1‑70 (MQ=255) tacctTCCAGCCCAATCGGAGCCTGAGAGTTATACCGCCCCAGCTGCGGTTAAAGTTACCGAAAATAGAta > 1:2432791/1‑71 (MQ=255) acctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta > 1:2439551/1‑70 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTTCGGTTAATGTTACCGATAATAGTta < 1:3063471/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGa < 1:3796863/60‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:5310379/69‑1 (MQ=255) cctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTta < 1:4763221/69‑1 (MQ=255) ctTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg > 1:1943673/1‑71 (MQ=255) ttCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTa > 1:815782/1‑54 (MQ=255) tCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAg > 1:1695969/1‑69 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTTCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:5113808/69‑1 (MQ=255) ccAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa < 1:5045414/70‑1 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:2144679/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:4094487/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTa > 1:1811317/1‑69 (MQ=255) cAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc < 1:2124118/70‑1 (MQ=255) ccccATCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc < 1:1764035/71‑1 (MQ=255) cccaTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGccc > 1:1181916/4‑71 (MQ=255) cAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGata > 1:3425088/1‑52 (MQ=255) cAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:28268/71‑1 (MQ=255) caTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTg < 1:2543541/70‑1 (MQ=255) aTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGa > 1:3363095/1‑71 (MQ=255) aTCGGGTCCTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCACTGa > 1:3547067/1‑71 (MQ=255) tCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAt > 1:2551937/1‑71 (MQ=255) tCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAt > 1:4835131/1‑71 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGataata > 1:4172927/1‑50 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:5074099/1‑70 (MQ=255) ggAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtc < 1:3677966/71‑1 (MQ=255) tccTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc < 1:5675327/70‑1 (MQ=255) tccTGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc < 1:2827269/70‑1 (MQ=255) ccTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatca > 1:1458927/1‑71 (MQ=255) cTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:1215386/71‑1 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGt < 1:1550200/52‑1 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTAc > 1:3606836/1‑54 (MQ=255) tGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat > 1:4255053/1‑70 (MQ=255) tGAGTAATATACCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctcat > 1:4848809/1‑70 (MQ=255) gggtgaTATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACtcctcatca < 1:3320751/69‑1 (MQ=255) gAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg < 1:5317765/44‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAg < 1:375271/43‑1 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca > 1:4364238/1‑70 (MQ=255) aGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTcatcatcat < 1:9140/71‑1 (MQ=255) gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:3075723/1‑61 (MQ=255) gtgtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGAtt > 1:1850727/1‑61 (MQ=255) gtgaTATACCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACtcctcat < 1:4888930/67‑1 (MQ=255) gtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:3995348/65‑1 (MQ=255) gtTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:3750460/65‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:5120786/54‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCt < 1:4608315/54‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTcatcatcat < 1:1729571/67‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact < 1:1963142/70‑1 (MQ=255) ttATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact < 1:704595/70‑1 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTGCCGGTGACGGTTATAGTACAGCCCCGACTCCTCATCAtgctg < 1:1260426/70‑2 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATtcctc > 1:2377207/2‑62 (MQ=255) atataCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCAtgctg > 1:5647503/2‑70 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTtata > 1:3483156/1‑42 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTtat > 1:4843679/1‑41 (MQ=255) ataCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATagtact > 1:311533/1‑71 (MQ=255) taCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGcc > 1:3598182/1‑49 (MQ=255) taCCGCCCCTGCAACGGATCGTAGTACCGGTGACGGTTATAGTACAGCCCTGATTCCTCATCATGctgctg > 1:1084867/1‑71 (MQ=255) aCCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:3216827/60‑1 (MQ=255) ccGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTActgct < 1:3679169/71‑1 (MQ=255) ccccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCg > 1:1675088/1‑71 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGa > 1:707945/1‑46 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGa > 1:4672802/1‑46 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCAtact < 1:4925133/60‑1 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATactactact < 1:2410494/66‑1 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCgg > 1:1158939/1‑70 (MQ=255) ccTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGc < 1:527883/71‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:3793395/55‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:1131489/55‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:2079703/55‑1 (MQ=255) ctgctgCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCa < 1:3059692/71‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:303116/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:4509804/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:4503456/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:1512071/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:105533/54‑1 (MQ=255) tgctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcatca < 1:3481780/54‑1 (MQ=255) gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:1960161/51‑1 (MQ=255) gctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATtcatcat < 1:946158/51‑1 (MQ=255) ctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGt < 1:4689544/70‑1 (MQ=255) ctgcGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTc > 1:67307/1‑71 (MQ=255) gTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtgatg < 1:3982140/71‑1 (MQ=255) ttAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtgat < 1:3604419/69‑1 (MQ=255) ttAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtgat < 1:5478436/69‑1 (MQ=255) ttAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtgat < 1:2148182/69‑1 (MQ=255) tAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGaa > 1:587680/1‑71 (MQ=255) tAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGaa > 1:610744/1‑71 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATactact > 1:5012677/1‑51 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATactact > 1:2881159/1‑51 (MQ=255) aaTGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGaa > 1:1630218/1‑70 (MQ=255) tGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtga > 1:1852637/1‑64 (MQ=255) tGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCtg < 1:1380358/63‑1 (MQ=255) | ATAAAAATTCCACCTACCTTCCAGCCCAATCGGAGCCTGAGTGTTATACCGCCCCTGCTGCGGTTAATGTTACCGATAATAGTTATAGTACAGCCCTGATTCATCATCATACTACTGCTGCGGCAGTCTGATGAA > minE/2572693‑2572827 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |