| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,572,941 | G→A | 84.6% | intergenic (+1709/‑70) | yibH → / → ysaB | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,572,941 | 0 | G | A | 84.6% | 176.9 / 22.9 | 78 | intergenic (+1709/‑70) | yibH/ysaB | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (5/7); new base A (34/32); total (39/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
AAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGT > minE/2572875‑2573004 | aaaTCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTc > 1:1169768/1‑71 (MQ=255)aaaTCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTc > 1:5595745/1‑71 (MQ=255)aaaTCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTc > 1:3026132/1‑71 (MQ=255)aaaTCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTc > 1:3004214/1‑71 (MQ=255) aTTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTaa < 1:272107/70‑1 (MQ=255) ttggttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGa > 1:4969468/1‑70 (MQ=255) tggttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGata > 1:1920790/1‑71 (MQ=255) ggttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGata < 1:3030054/70‑1 (MQ=255) ttggCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGt < 1:1525345/62‑1 (MQ=255) gcgcACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGc < 1:785101/70‑1 (MQ=255) tGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGa < 1:5088121/57‑1 (MQ=255) ggAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGc < 1:3418652/63‑1 (MQ=255) gAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCg > 1:3158720/1‑50 (MQ=255) gAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCa > 1:5616688/1‑60 (MQ=255) gAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCAc < 1:1312736/65‑1 (MQ=255) gAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATAcc > 1:125108/1‑71 (MQ=255) ataatCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGa > 1:4278370/1‑71 (MQ=255) atCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGata > 1:3104097/1‑70 (MQ=255) cTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTaa > 1:1915268/1‑46 (MQ=255) cTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTaa > 1:5615037/1‑46 (MQ=255) cTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTa > 1:1512673/1‑45 (MQ=255) tGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGa < 1:3625875/47‑1 (MQ=255) tGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGa < 1:1995288/47‑1 (MQ=255) gTTTTATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATataaataa < 1:1251632/71‑1 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTc > 1:377836/1‑35 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTa < 1:1831357/38‑1 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTa < 1:4704160/38‑1 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGat < 1:2575576/42‑1 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGat < 1:3343784/42‑1 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGat < 1:590753/42‑1 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGat < 1:2392739/42‑1 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGa > 1:2717102/1‑41 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGa > 1:4850804/1‑41 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAaataat > 1:5455667/1‑69 (MQ=255) ttATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAaataat > 1:1583118/1‑69 (MQ=255) tATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGat < 1:2713864/41‑1 (MQ=255) tATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCAc < 1:3780635/50‑1 (MQ=255) tATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCAc < 1:2642622/50‑1 (MQ=255) tATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAaataat > 1:2388573/1‑68 (MQ=255) tATCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAAtaataa < 1:4101268/70‑1 (MQ=255) aTCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGatat > 1:205479/1‑42 (MQ=255) aTCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCa > 1:5560245/1‑48 (MQ=255) aTCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATataaata > 1:813629/1‑65 (MQ=255) aTCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATataaata > 1:983441/1‑65 (MQ=255) tCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGAc > 1:2666502/1‑71 (MQ=255) tCACTACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGa < 1:4541958/70‑1 (MQ=255) tACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCAc < 1:3849703/44‑1 (MQ=255) tACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCAc < 1:5276767/44‑1 (MQ=255) tACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCAc < 1:4474490/44‑1 (MQ=255) tACAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCAc < 1:4783862/44‑1 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAc < 1:3300573/69‑1 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAc < 1:2417444/69‑1 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAc < 1:1254247/69‑1 (MQ=255) cAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACt < 1:1609978/70‑1 (MQ=255) cAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACta > 1:3514225/1‑44 (MQ=255) cAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGa > 1:2080281/1‑50 (MQ=255) cAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGa > 1:1074891/1‑50 (MQ=255) cAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGa > 1:2362670/1‑64 (MQ=255) cAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAcc < 1:4655347/70‑2 (MQ=255) cAGCTTATAGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAc < 1:5263584/69‑1 (MQ=255) cTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATAcc < 1:5386661/45‑1 (MQ=255) tataGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAcc < 1:2420840/65‑2 (MQ=255) tataGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCAc > 1:2460938/1‑64 (MQ=255) ataGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATataa < 1:5412653/49‑1 (MQ=255) taGAGGCTTAAGGAAATTCGTAAGATATCAGCCACtata > 1:5275196/1‑39 (MQ=255) taGAGGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACCTg > 1:4046264/1‑65 (MQ=255) agagGCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACCTGCAAAc > 1:2902988/1‑69 (MQ=255) gCTTAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACCTGCAAACCAGACg > 1:1505926/1‑71 (MQ=255) cTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACgg < 1:557743/71‑1 (MQ=255) ttAAGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACt > 1:1551219/1‑52 (MQ=255) aaGGAAATTCGTAAGATATCAGCCACTATACCGATataaa > 1:4351217/1‑40 (MQ=255) aaGGAAATTCGTAAGATATCAGCCACTATACCGATataaa > 1:2532043/1‑40 (MQ=255) aaGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGa > 1:2256599/1‑48 (MQ=255) aaGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGa > 1:2112574/1‑48 (MQ=255) aaGGAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACCTGCAAACCAGACGGt < 1:376872/69‑1 (MQ=255) aGGAAATTCGTAAGATATCAGCCACTATACCGATATAAAtaataa < 1:5513069/45‑1 (MQ=255) aGGAAATTCGTAAGATATCAGCCACTATACCGATATAAAtaataa < 1:584880/45‑1 (MQ=255) ggAAATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTc < 1:2601689/49‑1 (MQ=255) | AAATCATTTGGTTGGCGCACTGGAATAATCTGAGTTTTATCACTACAGCTTATAGAGGCTTAAGGAGATTCGTAAGATATCAGCCACTATACCGATATAAATAATAAGACTCACTTGCAAACCAGACGGT > minE/2572875‑2573004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |