| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,295 | A→G | 13.8% | G994G (GGT→GGC) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,295 | 0 | A | G | 13.8% | 210.9 / 22.3 | 87 | G994G (GGT→GGC) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (39/36); new base G (2/10); total (41/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01 | |||||||||||
AAATTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATT > minE/2584225‑2584361 | aaaTTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGaa > 1:2218682/1‑71 (MQ=255) aaTTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAAc > 1:2807942/1‑71 (MQ=255) aTTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAAcc < 1:956389/71‑1 (MQ=255) cAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCg > 1:4007632/1‑67 (MQ=255) gCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCTCCAGAACCGGCa > 1:1079461/1‑68 (MQ=255) gCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAAc > 1:1213764/1‑63 (MQ=255) gCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCa > 1:968101/1‑68 (MQ=255) cACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCAt < 1:2392213/71‑1 (MQ=255) cACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCAt < 1:2206919/71‑1 (MQ=255) aCTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCAt < 1:8923/70‑1 (MQ=255) tGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGa > 1:3745073/1‑70 (MQ=255) gTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGAc > 1:2874533/1‑70 (MQ=255) gTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACt > 1:3526394/1‑71 (MQ=255) ttGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACt > 1:4011988/1‑70 (MQ=255) ttGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTg < 1:5018544/71‑1 (MQ=255) gCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGa < 1:1577447/70‑1 (MQ=255) gCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGa < 1:3806177/70‑1 (MQ=255) gCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGa < 1:1727403/70‑1 (MQ=255) cGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGAt < 1:1188193/70‑1 (MQ=255) ggCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATaa < 1:3434867/71‑1 (MQ=255) gCAAACATCCCACCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc < 1:1517813/71‑1 (MQ=255) cAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:2414109/1‑70 (MQ=255) cAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc < 1:859642/70‑1 (MQ=255) cAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:3157592/1‑70 (MQ=255) aaaCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:2202186/1‑69 (MQ=255) aaaCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:3675681/1‑69 (MQ=255) aaCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAg > 1:1900021/1‑71 (MQ=255) aCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc < 1:2005434/71‑1 (MQ=255) cATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc < 1:3667268/70‑1 (MQ=255) cATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCg > 1:340559/1‑71 (MQ=255) aTCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAAc > 1:636662/1‑46 (MQ=255) aTCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGa > 1:2902715/1‑57 (MQ=255) cccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:2568325/1‑71 (MQ=255) cccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:3668171/1‑71 (MQ=255) ccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACg < 1:765931/71‑1 (MQ=255) cATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGc > 1:5622025/1‑71 (MQ=255) tCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGAt < 1:238504/52‑1 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:5471326/1‑68 (MQ=255) ccGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGaa > 1:1165698/1‑70 (MQ=255) ccGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGaa > 1:531115/1‑70 (MQ=255) cgatacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAAt > 1:1730401/4‑70 (MQ=9) cgatacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATa < 1:2237258/68‑1 (MQ=9) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGAc > 1:5354845/1‑42 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt > 1:1950990/1‑70 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:2445552/71‑1 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:5205726/71‑1 (MQ=255) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa < 1:1516042/71‑1 (MQ=255) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa > 1:2285179/1‑69 (MQ=255) atacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATaaa < 1:4123503/70‑1 (MQ=9) atacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATaaa < 1:852092/70‑1 (MQ=9) tacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAg < 1:4715207/71‑1 (MQ=9) cctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAg < 1:5339496/47‑1 (MQ=255) cctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGc > 1:3371844/1‑71 (MQ=255) accGCGTTTTGCGCGCCAGAACCGGCACCATGACTGa > 1:4548272/1‑37 (MQ=255) accGCGTTTTGAGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:384676/1‑53 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:1625428/1‑69 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:2544561/1‑69 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:1895414/71‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:4008447/71‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGCCAGCACGCCGAGAATAAAGGCCagag < 1:768882/71‑1 (MQ=255) agcgTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGcga > 1:4694479/2‑66 (MQ=9) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:1059270/1‑61 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:3908394/1‑61 (MQ=255) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:5171755/1‑61 (MQ=255) cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:5291444/1‑71 (MQ=255) gTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:4306235/67‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4521918/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4760626/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4764709/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGc < 1:4992602/62‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:5564802/66‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:562964/66‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa < 1:4791385/64‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa < 1:3214057/64‑1 (MQ=255) tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat > 1:391086/1‑71 (MQ=255) gcgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat < 1:3554749/70‑1 (MQ=255) cgcgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcatc < 1:1793153/70‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcatc < 1:4469410/70‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:4900481/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:1735021/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:129252/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:4289323/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGgcat < 1:4282731/69‑1 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:3756472/71‑1 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:503638/1‑71 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:5537871/1‑71 (MQ=255) ccAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt < 1:162097/71‑1 (MQ=255) cAGAACCGGCACCATGACTGATAACCAGAGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt > 1:365942/1‑71 (MQ=255) | AAATTGCCAGCACTGTTGCGGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATT > minE/2584225‑2584361 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |