| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,314 | A→G | 22.7% | V988A (GTT→GCT) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,314 | 0 | A | G | 22.7% | 248.5 / 62.3 | 119 | V988A (GTT→GCT) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (47/45); new base G (11/16); total (58/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
GGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGC > minE/2584244‑2584384 | ggCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATaa < 1:3434867/71‑1 (MQ=255) gCAAACATCCCACCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc < 1:1517813/71‑1 (MQ=255) cAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc < 1:859642/70‑1 (MQ=255) cAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:2414109/1‑70 (MQ=255) cAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:3157592/1‑70 (MQ=255) aaaCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:3675681/1‑69 (MQ=255) aaaCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAAc > 1:2202186/1‑69 (MQ=255) aCATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc < 1:2005434/71‑1 (MQ=255) cATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGc < 1:3667268/70‑1 (MQ=255) cATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCg > 1:340559/1‑71 (MQ=255) cccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:2568325/1‑71 (MQ=255) cccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:3668171/1‑71 (MQ=255) ccATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACg < 1:765931/71‑1 (MQ=255) aCGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc > 1:5471326/1‑68 (MQ=255) ccGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGaa > 1:531115/1‑70 (MQ=255) cgatacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAAt > 1:1730401/4‑70 (MQ=9) cgatacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATa < 1:2237258/68‑1 (MQ=9) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt > 1:1950990/1‑70 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:2445552/71‑1 (MQ=255) cGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa < 1:5205726/71‑1 (MQ=255) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa < 1:1516042/71‑1 (MQ=255) gTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATa > 1:2285179/1‑69 (MQ=255) atacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATaaa < 1:4123503/70‑1 (MQ=9) atacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATaaa < 1:852092/70‑1 (MQ=9) tacccacaGCGTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAg < 1:4715207/71‑1 (MQ=9) cctaccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAg < 1:5339496/47‑1 (MQ=255) accGCGTTTTGAGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCAc > 1:384676/1‑53 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:1625428/1‑69 (MQ=255) ccGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:2544561/1‑69 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:1895414/71‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagag < 1:4008447/71‑1 (MQ=255) cgcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGCCAGCACGCCGAGAATAAAGGCCagag < 1:768882/71‑1 (MQ=255) agcgTTCTGCGCGCCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGcga > 1:4694479/2‑66 (MQ=9) gcgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATaa > 1:1059270/1‑61 (MQ=255) cgTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagagag > 1:5291444/1‑71 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4764709/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4760626/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGcc < 1:4521918/50‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGc < 1:4992602/62‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:5564802/66‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCaga < 1:562964/66‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa < 1:4791385/64‑1 (MQ=255) ttttGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa < 1:3214057/64‑1 (MQ=255) tGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGtcat > 1:391086/1‑71 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcatc < 1:4469410/70‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:4289323/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:129252/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGtcat < 1:1735021/69‑1 (MQ=255) cgcgcCACTGCCGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGgcat < 1:4282731/69‑1 (MQ=255) cgcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:3756472/71‑1 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:503638/1‑71 (MQ=255) gcCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:5537871/1‑71 (MQ=255) ccAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAt < 1:162097/71‑1 (MQ=255) cAGAACCGGCACCATGACTGATAACCAGAGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGAtt > 1:365942/1‑71 (MQ=255) tgccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGAt < 1:4171865/65‑1 (MQ=12) gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCgaga > 1:4517874/2‑52 (MQ=255) gccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCgaga > 1:1120242/2‑52 (MQ=255) ccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg < 1:1165271/71‑1 (MQ=9) cGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg > 1:20626/1‑70 (MQ=9) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:4030879/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAAt < 1:2535918/38‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga < 1:1835747/50‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga < 1:4760639/50‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga < 1:3166582/50‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga < 1:4724158/50‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga < 1:5239277/50‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa > 1:3605538/1‑70 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa > 1:4600369/1‑70 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa > 1:2335523/1‑38 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa > 1:345050/1‑38 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa > 1:688751/1‑38 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATaaa > 1:4262358/1‑38 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg < 1:4370925/57‑1 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg < 1:3864851/71‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa < 1:5678795/57‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa < 1:4750877/57‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:1370591/71‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:2464338/71‑1 (MQ=255) gttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca > 1:5043168/4‑71 (MQ=2) gttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca < 1:544853/68‑1 (MQ=2) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCagaga > 1:1880683/1‑45 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCa > 1:1698327/1‑41 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGt > 1:3468221/1‑48 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAgg > 1:66371/1‑55 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTg > 1:4813082/1‑59 (MQ=255) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGACAGGTCATCAGGATTGGGCGTaa > 1:1121840/1‑66 (MQ=255) ttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg > 1:1321823/3‑68 (MQ=9) taCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGa > 1:2929012/2‑54 (MQ=17) aCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGt > 1:1137416/1‑45 (MQ=16) cTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcg < 1:602202/71‑1 (MQ=9) tGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgc < 1:3747188/59‑2 (MQ=9) tGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgc < 1:1845112/59‑2 (MQ=9) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGa > 1:2473156/1‑49 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:5475533/1‑60 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa > 1:4321275/1‑59 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt > 1:3998547/1‑65 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgcg > 1:4958936/1‑70 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCgcgc > 1:1885653/1‑71 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCgcgc > 1:3171747/1‑71 (MQ=255) aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc > 1:377027/1‑71 (MQ=255) | GGCAAACATCCCGCCCATCACGCCGGTACCTACCGCGTTTTGCGCGCCAGAACCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGC > minE/2584244‑2584384 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |