Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 311,870 T→G 15.9% I335L (ATC→CTC) ‡ acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE311,8700TG15.9% 198.5 / 34.5 94I335L (ATC→CTC) ‡acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (36/43);  new base G (8/7);  total (44/50)
Fisher's exact test for biased strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

AACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCT  >  minE/311800‑311938
                                                                      |                                                                    
 aCAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtt                                                                     >  1:4664282/1‑71 (MQ=255)
     aTACATAACGAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa                                                                 >  1:1882947/1‑71 (MQ=255)
       aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc                                                                >  1:4986943/1‑70 (MQ=255)
        cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa                                                              <  1:3941275/71‑1 (MQ=255)
        cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa                                                              <  1:2122378/71‑1 (MQ=255)
        cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa                                                              <  1:4763334/71‑1 (MQ=255)
        cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa                                                              <  1:1791893/71‑1 (MQ=255)
         aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa                                                                 >  1:4590069/1‑67 (MQ=255)
         aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTACGTGAATAGAGATTTTCa                                                                 >  1:4825532/1‑67 (MQ=255)
          tAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAc                                                            <  1:4887055/71‑1 (MQ=255)
               aGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt                                                       <  1:5323838/71‑1 (MQ=255)
               aGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt                                                       <  1:5056854/71‑1 (MQ=255)
                ggAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg                                                      >  1:1388699/1‑71 (MQ=255)
                ggAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg                                                      >  1:2177368/1‑71 (MQ=255)
                ggAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtg                                                      <  1:3748263/71‑1 (MQ=255)
                ggAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtg                                                      >  1:4970918/1‑71 (MQ=255)
                 gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt                                                                   <  1:326925/57‑1 (MQ=255)
                 gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt                                                                   <  1:1019905/57‑1 (MQ=255)
                 gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAt                                                                      >  1:2514016/1‑54 (MQ=255)
                 gAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtgg                                                     >  1:4428081/1‑71 (MQ=255)
                 gAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtgg                                                     >  1:5289143/1‑71 (MQ=255)
                  aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt                                                    <  1:2953439/71‑1 (MQ=255)
                  aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg                                                      >  1:4267877/1‑69 (MQ=255)
                  aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg                                                      >  1:3123258/1‑69 (MQ=255)
                  aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg                                                      >  1:2557573/1‑69 (MQ=255)
                  aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATATTCACGAACGGCgtg                                                      >  1:3374507/1‑69 (MQ=255)
                   acacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggtg                                                   <  1:4196140/71‑1 (MQ=255)
                   acacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggtg                                                   >  1:4649374/1‑71 (MQ=255)
                      cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt                                                                   >  1:1144417/1‑52 (MQ=255)
                      cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTc                                                                  >  1:4707099/1‑53 (MQ=255)
                      cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa                                                                 >  1:1715554/1‑54 (MQ=255)
                       gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                >  1:2362560/1‑70 (MQ=255)
                        aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa                                                                 >  1:1313830/1‑52 (MQ=255)
                        aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa                                              <  1:3480806/71‑1 (MQ=255)
                        aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGAGTGGTGTCGTa                                              >  1:2278972/1‑71 (MQ=255)
                            tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACg                                                           <  1:2902771/54‑1 (MQ=255)
                            tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt                                                       >  1:4893259/1‑58 (MQ=255)
                            tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATggg                                          >  1:3992616/1‑71 (MQ=255)
                             gatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg                                                               <  1:2354159/49‑1 (MQ=255)
                              atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAggg                                          >  1:3219919/4‑69 (MQ=12)
                              atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAggg                                          >  1:3060770/4‑69 (MQ=12)
                              atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa                                        <  1:1614961/68‑1 (MQ=16)
                              atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACTGCGTGGTGTCGTATGGGTa                                        <  1:2824688/71‑1 (MQ=255)
                               tggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAggg                                          >  1:132925/3‑68 (MQ=16)
                               tCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa                                                             >  1:2947451/1‑49 (MQ=255)
                                 gCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtg                                                      >  1:614230/1‑54 (MQ=255)
                                 gCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa                                        >  1:4444151/1‑68 (MQ=16)
                                  cTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAAc                                     >  1:3778978/1‑70 (MQ=255)
                                  cTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACa                                    <  1:5563301/71‑1 (MQ=255)
                                   ttCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa                                   >  1:3547085/1‑71 (MQ=255)
                                    tCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAt                                  >  1:4448835/1‑71 (MQ=255)
                                    tCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAt                                  >  1:3574996/1‑71 (MQ=255)
                                     cGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa                                   <  1:2911483/69‑1 (MQ=255)
                                       aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt                               <  1:4342279/71‑1 (MQ=255)
                                       aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt                               <  1:286767/71‑1 (MQ=255)
                                       aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt                               <  1:2827703/71‑1 (MQ=255)
                                       aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt                                >  1:5557755/1‑70 (MQ=255)
                                       aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGAAAACAAtttt                               <  1:1681204/71‑1 (MQ=255)
                                         cAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg                                                               <  1:5513063/37‑1 (MQ=255)
                                         cAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg                                                               <  1:2034661/37‑1 (MQ=255)
                                         cAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCa                             <  1:3497376/71‑1 (MQ=255)
                                          aGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                <  1:3461784/51‑1 (MQ=255)
                                           gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa                           <  1:1235295/71‑1 (MQ=255)
                                            cGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa                                              >  1:3057517/1‑51 (MQ=255)
                                              ttttTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTcat                                               <  1:4839119/48‑3 (MQ=12)
                                              ttttTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTcat                                               <  1:4251544/48‑3 (MQ=12)
                                              ttttTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTcat                                               <  1:1817670/48‑3 (MQ=12)
                                              ttttTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATaag                                            <  1:2968612/51‑3 (MQ=9)
                                              ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt                                <  1:742067/63‑1 (MQ=255)
                                              ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt                                <  1:2709341/63‑1 (MQ=255)
                                              ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtt                                 <  1:3855210/62‑1 (MQ=255)
                                              ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc                        <  1:2905741/71‑1 (MQ=255)
                                                ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt                                             >  1:4541384/1‑48 (MQ=255)
                                                ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt                                             >  1:1164868/1‑48 (MQ=255)
                                                  aaCCACTTCGTGAATAGAGATTTTCACGAACGGCgt                                                       <  1:4880805/36‑1 (MQ=255)
                                                  aaCCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                <  1:668516/43‑1 (MQ=255)
                                                  aaCCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                <  1:834660/43‑1 (MQ=255)
                                                  aaCCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACg                    >  1:3010050/1‑71 (MQ=255)
                                                   aCCACTTCGTGAATAGAGATTTTCACGAACGGCgt                                                       <  1:5637196/35‑1 (MQ=255)
                                                    ccACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                <  1:2750151/41‑1 (MQ=255)
                                                     cACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                <  1:439659/40‑1 (MQ=255)
                                                       cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                <  1:1360000/38‑1 (MQ=255)
                                                       cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                <  1:1152826/38‑1 (MQ=255)
                                                       cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTc                              >  1:3240829/1‑56 (MQ=255)
                                                       cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag               <  1:4687316/71‑1 (MQ=255)
                                                       cTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTAGAGAACCTTCATTCCCTGCGGgaag               <  1:1628984/71‑1 (MQ=255)
                                                        ttCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                <  1:3032935/37‑1 (MQ=255)
                                                          cGTGAATAGAGATTTTCACGAACGGCGTGGTGACGTATgg                                           >  1:2802816/1‑40 (MQ=255)
                                                           gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa                                              >  1:2875209/1‑36 (MQ=255)
                                                           gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt                                             >  1:1221363/1‑37 (MQ=255)
                                                           gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt                                             >  1:587779/1‑37 (MQ=255)
                                                                tAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTcc      <  1:1808024/71‑1 (MQ=255)
                                                                  gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCAt    >  1:743894/1‑71 (MQ=255)
                                                                    gaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCt  <  1:5619689/71‑1 (MQ=255)
                                                                      ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc                        <  1:1177609/47‑1 (MQ=255)
                                                                      ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc                        <  1:4379600/47‑1 (MQ=255)
                                                                      ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc                        <  1:3998361/47‑1 (MQ=255)
                                                                      ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc                        <  1:22871/47‑1 (MQ=255)
                                                                      ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc                        <  1:3293815/47‑1 (MQ=255)
                                                                      ttttCACGAACGGCGTGGTGTCGTATGGGCAAACAATTTTCAGAccc                        <  1:5313097/47‑1 (MQ=255)
                                                                      |                                                                    
AACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCT  >  minE/311800‑311938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: