| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,584,364 | G→A | 10.2% | R971R (CGC→CGT) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,584,364 | 0 | G | A | 10.2% | 194.0 / 8.4 | 68 | R971R (CGC→CGT) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (30/31); new base A (3/4); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
CCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATC > minE/2584296‑2584427 | ccGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg < 1:1165271/71‑1 (MQ=9)ccGGCAACGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg > 1:735007/1‑71 (MQ=9) cGGCACCGTTACTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACg > 1:20626/1‑70 (MQ=9) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:4030879/71‑1 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:4758177/71‑1 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:245938/71‑1 (MQ=255) ggCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa < 1:5418138/71‑1 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa > 1:3605538/1‑70 (MQ=255) gCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTa > 1:4600369/1‑70 (MQ=255) ccATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg < 1:3864851/71‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:1370591/71‑1 (MQ=255) cATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGc < 1:2464338/71‑1 (MQ=255) gttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca < 1:544853/68‑1 (MQ=2) gttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca > 1:5043168/4‑71 (MQ=2) aTGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGACAGGTCATCAGGATTGGGCGTaa > 1:1121840/1‑66 (MQ=255) ttaCTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACg > 1:1321823/3‑68 (MQ=9) cTGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACGCAGACGCATAcg < 1:602202/71‑1 (MQ=9) tGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgc < 1:3747188/59‑2 (MQ=9) tGATAGCTAGCGGTAATACGCCGAGAATAAAGGCGAGAGAGGTCATCAGGATAGGACgc < 1:1845112/59‑2 (MQ=9) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaaa > 1:5475533/1‑60 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTaa > 1:4321275/1‑59 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt > 1:3998547/1‑65 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCAt > 1:1528676/1‑65 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCcgcg > 1:4958936/1‑70 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCgcgc > 1:3171747/1‑71 (MQ=255) aTAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCgcgc > 1:1885653/1‑71 (MQ=255) aCCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGcgccgc > 1:377027/1‑71 (MQ=255) ccAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt < 1:3438055/71‑1 (MQ=255) ccAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt < 1:5679229/71‑1 (MQ=255) aGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc < 1:1230076/71‑1 (MQ=255) gCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTc > 1:1208272/1‑70 (MQ=255) ggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat < 1:2225644/71‑1 (MQ=255) ggCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCg > 1:2095808/1‑69 (MQ=255) gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg < 1:126222/71‑1 (MQ=255) gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg < 1:4232838/71‑1 (MQ=255) gcagcaCGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgatg < 1:774471/71‑1 (MQ=255) gCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATACGCGCCGCTTCgatga > 1:1508865/1‑65 (MQ=255) cGAGAATAAAGGCGAGAGAGGTCATCAGGATAGgacgcagacgca < 1:3597625/45‑1 (MQ=14) cGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCctct < 1:3884686/71‑1 (MQ=255) gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTa < 1:4799728/71‑1 (MQ=255) gagaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTa > 1:4662074/1‑71 (MQ=255) agaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCga > 1:481579/1‑58 (MQ=255) gaATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATc > 1:5569807/1‑71 (MQ=255) aaTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACg < 1:3102816/39‑1 (MQ=255) aTAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCgg < 1:970128/71‑1 (MQ=255) tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCt < 1:791186/50‑1 (MQ=255) tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGcc > 1:4039750/1‑62 (MQ=255) tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGcc > 1:2227228/1‑62 (MQ=255) tAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGc > 1:3886279/1‑71 (MQ=255) aaaGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCg > 1:83996/1‑71 (MQ=255) gCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGtttt < 1:866093/71‑1 (MQ=255) gCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGtttt < 1:2129364/71‑1 (MQ=255) ccAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCg > 1:873139/1‑66 (MQ=255) cAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc < 1:2919290/71‑1 (MQ=255) gagGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat < 1:2432651/44‑1 (MQ=255) gagGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat < 1:374103/44‑1 (MQ=255) gagGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCgat < 1:3840922/44‑1 (MQ=255) gTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt < 1:2522284/37‑1 (MQ=255) gTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt < 1:5193216/37‑1 (MQ=255) gTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt < 1:4719541/37‑1 (MQ=255) gTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCtt < 1:4915868/37‑1 (MQ=255) gTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCttcttt < 1:1855415/71‑1 (MQ=255) catcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGGTTGCCTCTATCGGCGTTTTCCCttctttct > 1:5203704/1‑71 (MQ=255) catcaGGATTGGGCGTAAACGCATCCGAGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCcttctt > 1:2094163/1‑68 (MQ=255) atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGttt > 1:3396711/1‑58 (MQ=255) atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc > 1:478239/1‑61 (MQ=255) atcaGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTcc > 1:1203596/1‑61 (MQ=255) caGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCct > 1:4589843/1‑61 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcat > 1:4278776/1‑71 (MQ=255) aGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcat > 1:5416896/1‑71 (MQ=255) ggATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGcatc < 1:2337921/71‑1 (MQ=255) gCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGca < 1:4661854/62‑1 (MQ=255) | CCGGCACCATGACTGATAACCAGCGGCAGCACGCCGAGAATAAAGGCCAGAGAGGTCATCAGGATTGGGCGTAAACGCATCCGCGCCGCTTCGATGATTGCCTCTATCGGCGTTTTCCCTTCTTTCTGCATC > minE/2584296‑2584427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |