| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,585,996 | C→T | 10.1% | P427P (CCG→CCA) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,585,996 | 0 | C | T | 10.1% | 188.6 / 9.0 | 69 | P427P (CCG→CCA) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (33/29); new base T (4/3); total (37/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
CGGAAAGAACAACGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCAC > minE/2585926‑2586061 | cGGAAAGAACAACGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGcttccttc > 1:2634283/1‑71 (MQ=255) ggAAAGAACAACGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGcttccttc < 1:2601162/70‑1 (MQ=255) aaGAACAACGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTcggcg > 1:620081/1‑71 (MQ=255) aaGAACAACGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTcgg < 1:3231872/69‑1 (MQ=255) aacaacGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTa < 1:4092427/71‑1 (MQ=255) caacGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGc > 1:532343/1‑71 (MQ=255) aacGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCt > 1:4973824/1‑71 (MQ=255) acGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCt > 1:1375659/1‑70 (MQ=255) cGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTa > 1:945911/1‑71 (MQ=255) cGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTa < 1:4823971/71‑1 (MQ=255) cGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTa > 1:5542459/1‑71 (MQ=255) gCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATc < 1:2724048/71‑1 (MQ=255) cAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCtt < 1:2220087/67‑1 (MQ=255) aaTACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCtt < 1:1138639/71‑1 (MQ=255) tACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGt < 1:377803/59‑1 (MQ=255) aCCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCg > 1:657695/1‑71 (MQ=255) aCCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCg > 1:3743496/1‑71 (MQ=255) ccGACCATCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGc < 1:4552762/71‑1 (MQ=255) ccGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCg > 1:2833422/1‑70 (MQ=255) cGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCa < 1:1240181/71‑1 (MQ=255) aCCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAAt < 1:2011006/71‑1 (MQ=255) gcACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACg > 1:1811148/1‑71 (MQ=255) aCGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCt < 1:125327/71‑1 (MQ=255) cGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATc > 1:430321/1‑53 (MQ=255) cGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCg < 1:4156064/58‑1 (MQ=255) cGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCaa > 1:1346624/1‑61 (MQ=255) cGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCaa > 1:1633482/1‑61 (MQ=255) cGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTc < 1:2813502/71‑1 (MQ=255) gTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTc > 1:5084550/1‑70 (MQ=255) gTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTc > 1:5446308/1‑70 (MQ=255) ttGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGa < 1:3118562/71‑1 (MQ=255) ggATCTGCCCCATCGATTTATGCGTCGCTTCCTTcggc > 1:4853883/1‑38 (MQ=255) ggATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGAc > 1:4162312/1‑70 (MQ=255) ggATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg > 1:2294056/1‑71 (MQ=255) gATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGt > 1:2823198/1‑71 (MQ=255) tGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGa < 1:3403794/64‑1 (MQ=255) ccccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc < 1:4277078/69‑1 (MQ=255) ccccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc < 1:3494037/69‑1 (MQ=255) cccATCGATTTATGCGTCGCTTCCTTCGGCGGTAg > 1:1647553/1‑35 (MQ=255) cccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGc > 1:3444262/1‑36 (MQ=255) gcATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCtt < 1:4186927/36‑1 (MQ=255) ccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccac < 1:2787915/70‑1 (MQ=255) cATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTa < 1:2503929/37‑1 (MQ=255) aTCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGAAACGCTCGACGTTCTccaccac > 1:4379646/1‑71 (MQ=255) tCGATTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTccaccacc < 1:5387773/71‑1 (MQ=255) tCGATTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTccaccacc < 1:4950397/71‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCTGTAGCTTATCTTCCGCAATGACACGCTCGACGTtc < 1:2722802/62‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTCTCTTCCGCAATGACACGCTCGACg < 1:422552/59‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATc > 1:1076301/1‑37 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCt < 1:1947130/38‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg < 1:4148434/59‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg < 1:1921339/59‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg < 1:2586622/59‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtc < 1:372526/62‑1 (MQ=255) cGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccacca > 1:5510911/1‑71 (MQ=255) cGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccacca > 1:4352232/1‑71 (MQ=255) gATTTATGCGTGGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:657686/1‑71 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGtt > 1:4465187/1‑59 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGtt > 1:1047006/1‑59 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:5107731/1‑71 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:2263492/1‑71 (MQ=255) aTTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCcaccaccact < 1:4525351/71‑2 (MQ=255) tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa < 1:3223445/71‑1 (MQ=255) tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa < 1:2794259/71‑1 (MQ=255) tATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATg > 1:665139/1‑71 (MQ=255) tATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATg > 1:2280330/1‑71 (MQ=255) aTGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGa > 1:299803/1‑41 (MQ=255) tGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATgg < 1:5116531/70‑1 (MQ=255) gCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGc > 1:3822126/1‑70 (MQ=255) cGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGa > 1:1823865/1‑47 (MQ=255) tCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATc > 1:2783153/1‑70 (MQ=255) tCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCa > 1:1131731/1‑71 (MQ=255) tCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCa > 1:2998804/1‑71 (MQ=255) gCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCatcatc > 1:2492488/1‑71 (MQ=12) cttcttttGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCcaccaccacta < 1:3240776/60‑3 (MQ=255) cttcttttGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCcaccaccacta < 1:5607836/60‑3 (MQ=255) ttccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcca > 1:2393417/1‑71 (MQ=255) tccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcca > 1:5090223/1‑70 (MQ=255) ccttcGGCGGTATCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:442400/70‑1 (MQ=255) ccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac > 1:3671983/1‑70 (MQ=255) tcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTcca > 1:1666011/1‑45 (MQ=255) tcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCa > 1:5576835/1‑62 (MQ=255) tcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCa > 1:4301458/1‑62 (MQ=255) | CGGAAAGAACAACGGCAATACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCAC > minE/2585926‑2586061 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |