| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,586,015 | G→A | 14.7% | A421V (GCG→GTG) ‡ | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,586,015 | 0 | G | A | 14.7% | 238.8 / 25.9 | 95 | A421V (GCG→GTG) ‡ | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (44/37); new base A (8/6); total (52/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
ACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAC > minE/2585945‑2586082 | aCCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCg > 1:657695/1‑71 (MQ=255)aCCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCg > 1:3743496/1‑71 (MQ=255) ccGACCATCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGc < 1:4552762/71‑1 (MQ=255) ccGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCg > 1:2833422/1‑70 (MQ=255) cGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCa < 1:1240181/71‑1 (MQ=255) aCCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAAt < 1:2011006/71‑1 (MQ=255) gcACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACg > 1:1811148/1‑71 (MQ=255) aCGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCt < 1:125327/71‑1 (MQ=255) cGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCg < 1:4156064/58‑1 (MQ=255) cGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCaa > 1:1346624/1‑61 (MQ=255) cGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCaa > 1:1633482/1‑61 (MQ=255) cGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTc < 1:2813502/71‑1 (MQ=255) gTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTc > 1:5084550/1‑70 (MQ=255) gTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTc > 1:5446308/1‑70 (MQ=255) ttGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGa < 1:3118562/71‑1 (MQ=255) ggATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGAc > 1:4162312/1‑70 (MQ=255) ggATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg > 1:2294056/1‑71 (MQ=255) gATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGt > 1:2823198/1‑71 (MQ=255) tGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGa < 1:3403794/64‑1 (MQ=255) ccccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc < 1:4277078/69‑1 (MQ=255) ccccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtctc < 1:3494037/69‑1 (MQ=255) ccATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccac < 1:2787915/70‑1 (MQ=255) aTCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGAAACGCTCGACGTTCTccaccac > 1:4379646/1‑71 (MQ=255) tCGATTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTccaccacc < 1:4950397/71‑1 (MQ=255) tCGATTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTccaccacc < 1:5387773/71‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCTGTAGCTTATCTTCCGCAATGACACGCTCGACGTtc < 1:2722802/62‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTCTCTTCCGCAATGACACGCTCGACg < 1:422552/59‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg < 1:4148434/59‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg < 1:2586622/59‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACg < 1:1921339/59‑1 (MQ=255) tCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTtc < 1:372526/62‑1 (MQ=255) cGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccacca > 1:5510911/1‑71 (MQ=255) cGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTccaccacca > 1:4352232/1‑71 (MQ=255) gATTTATGCGTGGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:657686/1‑71 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGtt > 1:1047006/1‑59 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGtt > 1:4465187/1‑59 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:2263492/1‑71 (MQ=255) gATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCcaccaccac > 1:5107731/1‑71 (MQ=255) aTTTTTCCGTCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCcaccaccact < 1:4525351/71‑2 (MQ=255) tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa < 1:2794259/71‑1 (MQ=255) tttATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGa < 1:3223445/71‑1 (MQ=255) tATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATg > 1:665139/1‑71 (MQ=255) tATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATg > 1:2280330/1‑71 (MQ=255) aTGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGa > 1:299803/1‑41 (MQ=255) tGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATgg < 1:5116531/70‑1 (MQ=255) gCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGc > 1:3822126/1‑70 (MQ=255) cGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGa > 1:1823865/1‑47 (MQ=255) tCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATc > 1:2783153/1‑70 (MQ=255) tCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCa > 1:2998804/1‑71 (MQ=255) tCGCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCa > 1:1131731/1‑71 (MQ=255) gCTTCTTTTGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCatcatc > 1:2492488/1‑71 (MQ=12) cttcttttGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCcaccaccacta < 1:3240776/60‑3 (MQ=255) cttcttttGGCGGGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCcaccaccacta < 1:5607836/60‑3 (MQ=255) ttccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcca > 1:2393417/1‑71 (MQ=255) tccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcca > 1:5090223/1‑70 (MQ=255) ccttcGGCGGTATCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:442400/70‑1 (MQ=255) ccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac > 1:3671983/1‑70 (MQ=255) ttggcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAg > 1:3930902/3‑70 (MQ=12) tcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTcca > 1:1666011/1‑45 (MQ=255) tcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCa > 1:5576835/1‑62 (MQ=255) tcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCa > 1:4301458/1‑62 (MQ=255) ggcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAgcag > 1:1569165/1‑68 (MQ=9) ggcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAgcag > 1:825352/1‑68 (MQ=9) ggcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAgcag > 1:1710291/1‑68 (MQ=9) gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACg < 1:605481/50‑1 (MQ=255) gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc < 1:3972463/71‑1 (MQ=255) gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc < 1:3005287/71‑1 (MQ=255) gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc < 1:3136815/71‑1 (MQ=255) gcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAgcag < 1:3445634/70‑4 (MQ=9) ggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAccc > 1:933768/1‑71 (MQ=255) gTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:444999/61‑1 (MQ=255) gTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:2818252/61‑1 (MQ=255) gTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:3232997/61‑1 (MQ=255) gTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:1864993/61‑1 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccacca > 1:4102139/1‑61 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTAt > 1:5560486/1‑71 (MQ=255) gCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATc < 1:2272792/55‑1 (MQ=255) gCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGAAACCCTATc > 1:2644426/1‑71 (MQ=255) cTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCg < 1:1158534/71‑1 (MQ=255) ttATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTc < 1:2589364/50‑1 (MQ=255) ttATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcca > 1:5611922/1‑55 (MQ=255) tATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccacca > 1:612211/1‑57 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGcc < 1:1981326/70‑1 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGcc < 1:1958845/70‑1 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGcc < 1:3455893/70‑1 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGcc < 1:5123431/70‑1 (MQ=255) ttCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCa > 1:2154697/1‑71 (MQ=255) tCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTAt > 1:1644244/1‑62 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCAt > 1:2994470/1‑45 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg > 1:5306042/1‑67 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg < 1:2701471/67‑1 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:4482433/1‑70 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:1496372/1‑70 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc < 1:3462431/70‑1 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:36240/1‑70 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:1000977/1‑70 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:727284/1‑70 (MQ=255) cGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccacca > 1:13833/1‑50 (MQ=255) cGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccacca > 1:5595408/1‑50 (MQ=255) | ACCGACCAGCGCACGTTGGATCTGCCCCATCGATTTATGCGTCGCTTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAC > minE/2585945‑2586082 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |