| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,586,059 | C→G | 7.6% | V406V (GTG→GTC) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,586,059 | 0 | C | G | 7.6% | 327.3 / 11.6 | 105 | V406V (GTG→GTC) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (48/49); new base G (4/4); total (52/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
TTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGA > minE/2585990‑2586127 | ttccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcca > 1:2393417/1‑71 (MQ=255) ccttcGGCGGTATCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:442400/70‑1 (MQ=255) ccttcGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac > 1:3671983/1‑70 (MQ=255) ttggcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAg > 1:3930902/3‑70 (MQ=12) ggcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAgcag > 1:825352/1‑68 (MQ=9) ggcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAgcag > 1:1710291/1‑68 (MQ=9) ggcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAgcag > 1:1569165/1‑68 (MQ=9) gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc < 1:3972463/71‑1 (MQ=255) gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc < 1:3136815/71‑1 (MQ=255) gcggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAc < 1:3005287/71‑1 (MQ=255) gcggGAGCTTATCCTCCATCATCACGCGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAgcag < 1:3445634/70‑4 (MQ=9) ggTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAccc > 1:933768/1‑71 (MQ=255) gTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:444999/61‑1 (MQ=255) gTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:1864993/61‑1 (MQ=255) gTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccac < 1:3232997/61‑1 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccacca > 1:4102139/1‑61 (MQ=255) aGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTAt > 1:5560486/1‑71 (MQ=255) gCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGAAACCCTATc > 1:2644426/1‑71 (MQ=255) cTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCg < 1:1158534/71‑1 (MQ=255) ttATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATcca > 1:5611922/1‑55 (MQ=255) tATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccacca > 1:612211/1‑57 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGcc < 1:1981326/70‑1 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGcc < 1:5123431/70‑1 (MQ=255) aTCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGcc < 1:3455893/70‑1 (MQ=255) ttCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCa > 1:2154697/1‑71 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg < 1:2701471/67‑1 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAg > 1:5306042/1‑67 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:36240/1‑70 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc < 1:3462431/70‑1 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:727284/1‑70 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:4482433/1‑70 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:1496372/1‑70 (MQ=255) ccGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAc > 1:1000977/1‑70 (MQ=255) cGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccacca > 1:5595408/1‑50 (MQ=255) cGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATccacca > 1:13833/1‑50 (MQ=255) aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAAcc > 1:487722/1‑53 (MQ=255) aaTGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCCTc > 1:2445586/1‑70 (MQ=255) tGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCg < 1:239552/71‑1 (MQ=255) gACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCg > 1:3710229/1‑70 (MQ=255) gACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCg > 1:513850/1‑70 (MQ=255) gACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCg > 1:4479163/1‑70 (MQ=255) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTa < 1:3211752/52‑1 (MQ=255) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCa > 1:4387819/1‑64 (MQ=255) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCa > 1:2238589/1‑64 (MQ=255) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGaa < 1:2038152/71‑1 (MQ=255) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGaa < 1:902688/71‑1 (MQ=255) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGaa > 1:1026468/1‑71 (MQ=255) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGa < 1:4655363/70‑1 (MQ=255) acacGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGa < 1:1874588/70‑1 (MQ=255) gcGCTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACa < 1:159455/70‑1 (MQ=2) acGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATc > 1:243287/1‑52 (MQ=255) acGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa < 1:3496881/71‑1 (MQ=255) acGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa < 1:3254561/71‑1 (MQ=255) acGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa < 1:5507608/71‑1 (MQ=255) acGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa > 1:610043/1‑71 (MQ=255) gCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACAt < 1:3537085/70‑1 (MQ=255) cTCGACGTTCTCCACCACCACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACAtcg < 1:602302/71‑3 (MQ=2) cTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAg > 1:3221352/1‑71 (MQ=255) cGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGt > 1:5515794/1‑70 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTATCCCTATCGCCAGCACCTTCCCg < 1:3733546/61‑1 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc < 1:5561870/70‑1 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc > 1:1944879/1‑70 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc < 1:849761/70‑1 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc > 1:5289962/1‑70 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc > 1:5310107/1‑70 (MQ=255) gACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCa < 1:5507873/71‑1 (MQ=255) gACGTTCTCCACCACCACGATGGCATCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCa < 1:3569061/71‑1 (MQ=255) aCGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTc < 1:146853/69‑1 (MQ=255) tctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgt < 1:2751877/70‑1 (MQ=255) ctcCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCaa > 1:4981563/1‑66 (MQ=255) ccaccaccaCGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGtt > 1:1307566/1‑70 (MQ=255) ccaccaccaCGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGtt < 1:4429117/70‑1 (MQ=255) ccaccaccaCGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGtt < 1:11034/70‑1 (MQ=255) accaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGAt > 1:5508989/1‑71 (MQ=255) ccaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAcc > 1:2825773/1‑44 (MQ=255) ccaccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCAcc > 1:3903108/1‑44 (MQ=255) caccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATcc > 1:2570334/1‑47 (MQ=255) caccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATcc > 1:1357341/1‑47 (MQ=255) caccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAAc < 1:2948496/52‑1 (MQ=255) accacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGt < 1:1407205/71‑1 (MQ=255) accacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGt < 1:1467012/71‑1 (MQ=255) accacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGt < 1:914571/71‑1 (MQ=255) ccacGATGGCGTCATCCACCAGTAACCCTATCGcc < 1:5219577/35‑1 (MQ=255) ccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCAt > 1:5176160/1‑43 (MQ=255) ccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGa < 1:2856327/48‑1 (MQ=255) ccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa > 1:3947346/1‑51 (MQ=255) ccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACa > 1:1259390/1‑51 (MQ=255) ccacGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACAt > 1:4855156/1‑52 (MQ=255) acGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGa > 1:5439424/1‑70 (MQ=255) aTGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAacc < 1:5213378/71‑1 (MQ=255) tGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGa < 1:4520277/66‑1 (MQ=255) tGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAacc > 1:2316960/1‑70 (MQ=255) gCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAaccga < 1:266197/70‑1 (MQ=255) cATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAaccga > 1:1072437/1‑66 (MQ=255) aTCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGt < 1:4471326/42‑1 (MQ=255) aTCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACgtgt > 1:4056023/1‑50 (MQ=255) aTCCACCAGTAACCCTATCGCCAGCACCATCACGAACATAGTCAACgtgt > 1:5002358/1‑50 (MQ=255) tCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGa < 1:1654899/71‑1 (MQ=255) ccaccaGTAACCCTATCGCCAGCACCATCCCGAACATAGTCa < 1:5265999/42‑1 (MQ=255) caccaGTAACCCTATCGCCAGCACCATCCCGAACATa > 1:5619273/1‑37 (MQ=255) | TTCCTTCGGCGGTAGCTTATCTTCCGCAATGACACGCTCGACGTTCTCCACCACCACGATGGCGTCATCCACCAGTAACCCTATCGCCAGCACCATCCCGAACATAGTCAACGTGTTGATGGTGAAACCGACCGCCGA > minE/2585990‑2586127 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |