| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,873 | T→G | 16.0% | K334Q (AAA→CAA) ‡ | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,873 | 0 | T | G | 16.0% | 201.7 / 36.5 | 94 | K334Q (AAA→CAA) ‡ | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (36/43); new base G (8/7); total (44/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 105 total aligned reads displayed.
ATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCT > minE/311805‑311938 | aTACATAACGAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa > 1:1882947/1‑71 (MQ=255) aCATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCAc > 1:4986943/1‑70 (MQ=255) cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa < 1:2122378/71‑1 (MQ=255) cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa < 1:1791893/71‑1 (MQ=255) cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGa < 1:4763334/71‑1 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa > 1:4590069/1‑67 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTACGTGAATAGAGATTTTCa > 1:4825532/1‑67 (MQ=255) tAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAc < 1:4887055/71‑1 (MQ=255) aGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:5323838/71‑1 (MQ=255) aGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:5056854/71‑1 (MQ=255) ggAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg > 1:1388699/1‑71 (MQ=255) ggAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg > 1:2177368/1‑71 (MQ=255) ggAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtg > 1:4970918/1‑71 (MQ=255) ggAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtg < 1:3748263/71‑1 (MQ=255) gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt < 1:326925/57‑1 (MQ=255) gAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt < 1:1019905/57‑1 (MQ=255) gAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtgg > 1:4428081/1‑71 (MQ=255) gAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtgg > 1:5289143/1‑71 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt > 1:3048319/1‑71 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt < 1:2953439/71‑1 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg > 1:4267877/1‑69 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg > 1:3123258/1‑69 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg > 1:2557573/1‑69 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATATTCACGAACGGCgtg > 1:3374507/1‑69 (MQ=255) acacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggtg < 1:4196140/71‑1 (MQ=255) acacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggtg > 1:4649374/1‑71 (MQ=255) acGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGgtgt < 1:1719512/70‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt > 1:1144417/1‑52 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTc > 1:4707099/1‑53 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa > 1:1715554/1‑54 (MQ=255) gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg > 1:2362560/1‑70 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa > 1:1313830/1‑52 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa < 1:3480806/71‑1 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGAGTGGTGTCGTa > 1:2278972/1‑71 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACg < 1:2902771/54‑1 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt > 1:4893259/1‑58 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATggg > 1:3992616/1‑71 (MQ=255) gatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg < 1:2354159/49‑1 (MQ=255) atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAggg > 1:3060770/4‑69 (MQ=12) atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAggg > 1:3219919/4‑69 (MQ=12) atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa < 1:1614961/68‑1 (MQ=16) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACTGCGTGGTGTCGTATGGGTa < 1:2824688/71‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTa < 1:3785131/71‑1 (MQ=255) tggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAggg > 1:132925/3‑68 (MQ=16) tCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa > 1:2947451/1‑49 (MQ=255) gCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCgtg > 1:614230/1‑54 (MQ=255) gCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa > 1:4444151/1‑68 (MQ=16) cTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAAc > 1:3778978/1‑70 (MQ=255) cTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACa < 1:5563301/71‑1 (MQ=255) ttCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa > 1:3547085/1‑71 (MQ=255) tCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAt > 1:3574996/1‑71 (MQ=255) tCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAt > 1:4448835/1‑71 (MQ=255) cGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa < 1:2911483/69‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:286767/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:4342279/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:2827703/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt > 1:5557755/1‑70 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGAAAACAAtttt < 1:1681204/71‑1 (MQ=255) cAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg < 1:5513063/37‑1 (MQ=255) cAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCa < 1:3497376/71‑1 (MQ=255) aGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:3461784/51‑1 (MQ=255) gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa < 1:1235295/71‑1 (MQ=255) gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa < 1:5143234/71‑1 (MQ=255) cGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:3057517/1‑51 (MQ=255) cGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:2735792/1‑51 (MQ=255) ttttTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTcat < 1:4251544/48‑3 (MQ=12) ttttTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTcat < 1:4839119/48‑3 (MQ=12) ttttTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTcat < 1:1817670/48‑3 (MQ=12) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt < 1:2709341/63‑1 (MQ=255) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt < 1:742067/63‑1 (MQ=255) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtt < 1:3855210/62‑1 (MQ=255) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:2905741/71‑1 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:1164868/1‑48 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:4541384/1‑48 (MQ=255) aaCCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:668516/43‑1 (MQ=255) aaCCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACg > 1:3010050/1‑71 (MQ=255) aCCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:5637196/35‑1 (MQ=255) ccACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:2750151/41‑1 (MQ=255) cACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:439659/40‑1 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:1360000/38‑1 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:1152826/38‑1 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTc > 1:3240829/1‑56 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag < 1:4687316/71‑1 (MQ=255) cTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTAGAGAACCTTCATTCCCTGCGGgaag < 1:1628984/71‑1 (MQ=255) ttCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:3032935/37‑1 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGACGTATgg > 1:2802816/1‑40 (MQ=255) gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:2875209/1‑36 (MQ=255) gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:587779/1‑37 (MQ=255) gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:1221363/1‑37 (MQ=255) tAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTcc < 1:1808024/71‑1 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCAt > 1:743894/1‑71 (MQ=255) gaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCt < 1:5619689/71‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:3293815/47‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:22871/47‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:3998361/47‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:4379600/47‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:1177609/47‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGCAAACAATTTTCAGAccc < 1:5313097/47‑1 (MQ=255) ttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa > 1:4099146/1‑42 (MQ=255) ttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa > 1:5069204/1‑42 (MQ=255) | ATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCT > minE/311805‑311938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |