| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,350 | C→G | 22.9% | V416V (GTG→GTC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,350 | 0 | C | G | 22.9% | 211.7 / 59.0 | 109 | V416V (GTG→GTC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base C (34/50); new base G (13/12); total (47/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 114 total aligned reads displayed.
TGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTC > minE/2595280‑2595419 | tGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc < 1:5228961/71‑1 (MQ=255) aaTGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca > 1:1251148/1‑70 (MQ=255) ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGGGCAGCCAGCGTCACAAACAg > 1:5265201/1‑71 (MQ=255) ttGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg > 1:287879/1‑71 (MQ=255) ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa < 1:1086304/70‑1 (MQ=255) ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa < 1:3899196/70‑1 (MQ=255) ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa < 1:4281743/70‑1 (MQ=255) ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACa < 1:5067645/70‑1 (MQ=255) cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac < 1:2572734/71‑1 (MQ=255) cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac < 1:844464/71‑1 (MQ=255) cTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca < 1:1061871/70‑1 (MQ=255) cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGcaccg > 1:4169565/1‑69 (MQ=255) atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa < 1:541780/64‑1 (MQ=255) atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa < 1:5688686/64‑1 (MQ=255) atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa < 1:2459377/64‑1 (MQ=255) atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa < 1:2809995/64‑1 (MQ=255) atatCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcagg > 1:4622462/1‑71 (MQ=255) atCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa > 1:2065172/1‑62 (MQ=255) tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTc > 1:2403905/1‑54 (MQ=255) tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag > 1:4892038/1‑71 (MQ=255) tCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggca < 1:1831062/70‑1 (MQ=255) aGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAggg < 1:977500/71‑1 (MQ=255) tATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGca < 1:3053832/60‑1 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTcaca < 1:2242025/51‑1 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACaaa > 1:4709551/1‑53 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg > 1:3134278/1‑56 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAg > 1:705235/1‑56 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag < 1:4290259/65‑1 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag < 1:3355540/65‑1 (MQ=255) tctcATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt > 1:65953/1‑71 (MQ=255) tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCAccggcaca > 1:3592595/1‑64 (MQ=255) tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt > 1:1160607/1‑71 (MQ=255) tctaATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCAt > 1:1518678/1‑71 (MQ=255) aaTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGa > 1:1664135/2‑70 (MQ=255) aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac < 1:3521973/70‑2 (MQ=255) aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac > 1:4377325/1‑69 (MQ=255) aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGac > 1:1737157/1‑69 (MQ=255) aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATg < 1:2284194/68‑1 (MQ=255) aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGAt < 1:4851837/70‑1 (MQ=255) aTTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa < 1:5540100/71‑1 (MQ=255) ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACa < 1:4860878/50‑1 (MQ=255) ttACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATaa < 1:308626/71‑1 (MQ=255) tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAt > 1:5577178/1‑71 (MQ=255) tACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcag < 1:5680226/59‑1 (MQ=255) aCAAAGTGAAAGAGAGACGTAGCGCCAGCTCAGCCAGCGTCACAAAc > 1:1909327/1‑47 (MQ=255) aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa > 1:4675637/1‑66 (MQ=255) aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc > 1:2982070/1‑71 (MQ=255) aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc > 1:2094823/1‑71 (MQ=255) cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc < 1:543292/71‑1 (MQ=255) cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc > 1:1603561/1‑71 (MQ=255) aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac < 1:1261978/50‑1 (MQ=255) aaaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGcac < 1:4110322/50‑1 (MQ=255) aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg > 1:2154353/1‑56 (MQ=255) aaGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggcagg > 1:3923040/1‑56 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:4786190/71‑1 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:3202795/71‑1 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:1826622/71‑1 (MQ=255) gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg > 1:5242204/1‑68 (MQ=255) gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:1153276/1‑70 (MQ=255) gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGt < 1:969119/71‑1 (MQ=255) agagagACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATa > 1:2000060/1‑58 (MQ=255) gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg > 1:825321/1‑70 (MQ=255) gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg > 1:1827920/1‑70 (MQ=255) gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:163546/71‑1 (MQ=255) gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:5453997/71‑1 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:5410098/71‑1 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:1321587/71‑1 (MQ=255) tAGCGCCAGCGCAGCCAGCGTCACAAACAGCAcaggc < 1:1791825/37‑1 (MQ=255) cacgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcc > 1:5377867/3‑71 (MQ=255) aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt > 1:1784388/1‑50 (MQ=255) aGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAAt > 1:5171048/1‑50 (MQ=255) gcgcCCGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt > 1:1979192/1‑43 (MQ=255) gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCAt > 1:83857/1‑43 (MQ=255) gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGa > 1:3131452/1‑45 (MQ=255) gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:5127962/1‑71 (MQ=255) gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:5591103/1‑71 (MQ=255) acgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc > 1:2590656/2‑71 (MQ=255) cgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc < 1:3471919/71‑1 (MQ=255) ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAg > 1:4473558/1‑71 (MQ=255) gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc < 1:4704743/66‑1 (MQ=255) gcgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt > 1:2839713/1‑70 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:29933/71‑1 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:513441/71‑1 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:1243161/71‑1 (MQ=255) cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt < 1:2041747/44‑1 (MQ=255) cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:5070701/1‑69 (MQ=255) cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:3649646/1‑69 (MQ=255) ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg > 1:3870623/2‑69 (MQ=255) gccagaGTGACAAACAGCACCGGCACAGTCATGac < 1:2503828/35‑2 (MQ=12) gccagaGTGACAAACAGCACCGGCACAGTCATGac < 1:584133/35‑2 (MQ=12) cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa < 1:214073/71‑1 (MQ=255) agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa < 1:1674147/70‑1 (MQ=255) agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:5646237/1‑70 (MQ=255) agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa < 1:396040/70‑1 (MQ=255) cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:1256084/71‑1 (MQ=255) gTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:2985680/70‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:4270512/60‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:1595552/60‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc < 1:1791542/71‑1 (MQ=255) cacaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc > 1:5575767/1‑64 (MQ=255) | TGGTAATGTTGCTCATATCAGTATCTCATTACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTC > minE/2595280‑2595419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |