| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,380 | C→G | 28.8% | G406G (GGG→GGC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,380 | 0 | C | G | 28.8% | 175.8 / 85.9 | 118 | G406G (GGG→GGC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base C (41/43); new base G (18/16); total (59/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
ACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGC > minE/2595310‑2595449 | aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc > 1:2094823/1‑71 (MQ=255)aCAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATc > 1:2982070/1‑71 (MQ=255) cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc < 1:543292/71‑1 (MQ=255) cAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATcc > 1:1603561/1‑71 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:1826622/71‑1 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:4669639/71‑1 (MQ=255) aGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg < 1:4786190/71‑1 (MQ=255) gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATgccgg > 1:5242204/1‑68 (MQ=255) gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:1153276/1‑70 (MQ=255) gTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTg > 1:5256164/1‑70 (MQ=255) gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg > 1:1827920/1‑70 (MQ=255) gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAg > 1:825321/1‑70 (MQ=255) gagaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGt < 1:1696360/71‑1 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:1321587/71‑1 (MQ=255) agaCGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTa < 1:5410098/71‑1 (MQ=255) gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:5591103/1‑71 (MQ=255) gcgcCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATccc > 1:5127962/1‑71 (MQ=255) acgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc > 1:3025161/2‑71 (MQ=255) cgcCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc < 1:3471919/71‑1 (MQ=255) aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt < 1:130726/71‑1 (MQ=255) gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc < 1:4704743/66‑1 (MQ=255) gcgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt > 1:2839713/1‑70 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:513441/71‑1 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:29933/71‑1 (MQ=255) cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCt < 1:2041747/44‑1 (MQ=255) cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:5070701/1‑69 (MQ=255) cagccagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa > 1:3649646/1‑69 (MQ=255) ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg > 1:3870623/2‑69 (MQ=255) cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGCTAGTCa < 1:5555/71‑1 (MQ=255) agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa < 1:1674147/70‑1 (MQ=255) agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:5646237/1‑70 (MQ=255) gcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata < 1:4893225/71‑1 (MQ=255) cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:4148128/71‑1 (MQ=255) cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:614030/71‑1 (MQ=255) cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:1256084/71‑1 (MQ=255) gTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:2985680/70‑1 (MQ=255) tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt < 1:1203956/69‑2 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:4270512/60‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:1595552/60‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc < 1:1791542/71‑1 (MQ=255) cacaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc > 1:5575767/1‑64 (MQ=255) acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt < 1:3852905/71‑1 (MQ=255) acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttt < 1:2152288/70‑3 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa > 1:2766781/1‑41 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGaa > 1:1231302/1‑41 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATcc > 1:24804/1‑50 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAg > 1:2919375/1‑54 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:4352398/1‑71 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:4167808/1‑71 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGAACGGAAGTAATATcc > 1:5395728/1‑50 (MQ=255) caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc < 1:3068514/71‑1 (MQ=255) aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct > 1:4119604/1‑71 (MQ=255) aCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc > 1:4643370/1‑71 (MQ=255) cagcagAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc > 1:5555800/1‑70 (MQ=255) aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg > 1:74608/1‑68 (MQ=255) aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggg > 1:4332141/1‑68 (MQ=255) acaGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATtcttct > 1:34713/1‑64 (MQ=255) aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt < 1:2549641/51‑1 (MQ=255) aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa > 1:1731795/1‑71 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc < 1:1130225/71‑1 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc < 1:4430395/71‑1 (MQ=255) gcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACg < 1:5252685/71‑1 (MQ=255) caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt < 1:4120927/71‑1 (MQ=255) caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt < 1:1486229/71‑1 (MQ=255) aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata > 1:3604778/1‑51 (MQ=255) aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata > 1:2803758/1‑51 (MQ=255) acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCAc < 1:1809577/65‑1 (MQ=255) acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg > 1:1389504/4‑71 (MQ=255) acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:555069/68‑1 (MQ=255) acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:5285263/68‑1 (MQ=255) gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc < 1:1524422/71‑1 (MQ=255) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCa < 1:3979752/46‑1 (MQ=16) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:2483560/68‑1 (MQ=255) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc < 1:725182/69‑1 (MQ=255) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc < 1:52761/69‑1 (MQ=255) tgTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc < 1:2257996/65‑1 (MQ=255) ggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc < 1:3415756/52‑1 (MQ=255) tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa < 1:5615276/59‑1 (MQ=255) tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc > 1:4912920/1‑71 (MQ=255) tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt < 1:4769993/50‑2 (MQ=255) tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg > 1:2554648/1‑61 (MQ=255) tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg > 1:774505/1‑61 (MQ=255) aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc > 1:4134779/1‑42 (MQ=255) aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc > 1:431553/1‑42 (MQ=255) aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa < 1:5321996/71‑1 (MQ=255) aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg > 1:833614/1‑65 (MQ=255) aTGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCa < 1:203005/71‑1 (MQ=255) tGATAATCCCTGTACGGAAGTAATATCCCCAGCTg < 1:3026791/35‑1 (MQ=255) tGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata < 1:4453964/44‑1 (MQ=255) aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:2099194/1‑47 (MQ=255) aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:3303398/1‑47 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt > 1:700295/1‑37 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:5424356/1‑55 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:4243826/1‑55 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:470386/1‑55 (MQ=255) aaTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg > 1:2184508/1‑48 (MQ=19) tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc > 1:4444805/1‑71 (MQ=255) cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:5274802/1‑68 (MQ=255) cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:2801405/1‑68 (MQ=255) cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:4924942/1‑68 (MQ=255) | ACAAAGTGAAAGAGAGACGTAGCGCCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGC > minE/2595310‑2595449 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |