Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 2,595,404 | G→A | 29.4% | S398S (AGC→AGT) ‡ | arsB ← | arsenite/antimonite transporter |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 2,595,404 | 0 | G | A | 29.4% | 196.8 / 97.1 | 136 | S398S (AGC→AGT) ‡ | arsB | arsenite/antimonite transporter |
Reads supporting (aligned to +/- strand): ref base G (51/45); new base A (18/22); total (69/67) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.53e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 141 total aligned reads displayed.
CCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAA > minE/2595334‑2595474 | ccAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAg > 1:4473558/1‑71 (MQ=255) aGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt < 1:130726/71‑1 (MQ=255) gcgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCt > 1:2839713/1‑70 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:1243161/71‑1 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:29933/71‑1 (MQ=255) cgcAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:513441/71‑1 (MQ=255) ggccagaGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAttg > 1:3870623/2‑69 (MQ=255) cagcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa < 1:214073/71‑1 (MQ=255) agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa > 1:5646237/1‑70 (MQ=255) agcGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCa < 1:1674147/70‑1 (MQ=255) cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:614030/71‑1 (MQ=255) cGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:4148128/71‑1 (MQ=255) gTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCatat < 1:2985680/70‑1 (MQ=255) tgacaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt < 1:1203956/69‑2 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGa < 1:4270512/60‑1 (MQ=255) tCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc < 1:1791542/71‑1 (MQ=255) cacaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc > 1:5575767/1‑64 (MQ=255) acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt < 1:3852905/71‑1 (MQ=255) acaAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttt < 1:2152288/70‑3 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAg > 1:2919375/1‑54 (MQ=255) acaAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:4352398/1‑71 (MQ=255) acaAACAGCAAAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:2334813/1‑71 (MQ=255) caAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttttc < 1:3068514/71‑1 (MQ=255) aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct > 1:1942030/1‑71 (MQ=255) aaaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATttttct > 1:760969/1‑71 (MQ=255) aaCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTg > 1:1783083/1‑71 (MQ=255) aCAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc > 1:4643370/1‑71 (MQ=255) cagcagAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGc > 1:5555800/1‑70 (MQ=255) aggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt < 1:2549641/51‑1 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc < 1:1130225/71‑1 (MQ=255) ggcaggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTAc < 1:4430395/71‑1 (MQ=255) caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt < 1:1486229/71‑1 (MQ=255) caggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGt < 1:4120927/71‑1 (MQ=255) aggGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCata > 1:3604778/1‑51 (MQ=255) acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:5285263/68‑1 (MQ=255) acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:555069/68‑1 (MQ=255) acagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg > 1:1389504/4‑71 (MQ=255) gggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGc < 1:1524422/71‑1 (MQ=255) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCa < 1:3979752/46‑1 (MQ=16) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:2483560/68‑1 (MQ=255) cagTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGc < 1:725182/69‑1 (MQ=255) ggTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttc < 1:3415756/52‑1 (MQ=255) tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGaa < 1:5615276/59‑1 (MQ=255) tCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGc > 1:4912920/1‑71 (MQ=255) tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt < 1:4769993/50‑2 (MQ=255) tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg > 1:2554648/1‑61 (MQ=255) tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg > 1:774505/1‑61 (MQ=255) cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtt > 1:5272059/1‑48 (MQ=19) cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc > 1:3535977/1‑70 (MQ=255) aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc > 1:431553/1‑42 (MQ=255) aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTc > 1:4134779/1‑42 (MQ=255) aTGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa < 1:5321996/71‑1 (MQ=255) tGATAATCCCTGTACGGAAGTAATATCCCCAGCTg < 1:3026791/35‑1 (MQ=255) aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATAttctt > 1:4746272/1‑47 (MQ=255) aTAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCagag < 1:5564881/71‑1 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGt > 1:700295/1‑37 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:470386/1‑55 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:4243826/1‑55 (MQ=255) tAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAg > 1:5424356/1‑55 (MQ=255) aaTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg > 1:2184508/1‑48 (MQ=19) tCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc > 1:4444805/1‑71 (MQ=255) cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:4924942/1‑68 (MQ=255) cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:516239/1‑68 (MQ=255) cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:5274802/1‑68 (MQ=255) cccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgta > 1:2801405/1‑68 (MQ=255) ccTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg > 1:4551306/1‑70 (MQ=255) cggTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagtt < 1:5530447/69‑2 (MQ=255) tGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag > 1:3436204/1‑71 (MQ=255) gTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtagc > 1:4700516/1‑71 (MQ=255) gTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCAcat < 1:2890320/52‑3 (MQ=255) tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa > 1:4353467/1‑48 (MQ=255) tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTa > 1:1377180/1‑48 (MQ=255) tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCa < 1:1988895/58‑1 (MQ=255) tACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcgtag < 1:846704/69‑1 (MQ=255) aCGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAgcagagttgc > 1:4250600/1‑69 (MQ=255) cGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAg > 1:1359458/1‑71 (MQ=255) ggAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAgg > 1:2068162/1‑71 (MQ=255) aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:4607899/1‑70 (MQ=255) aaGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGc > 1:1061447/1‑70 (MQ=255) aaGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCac < 1:4735523/54‑2 (MQ=255) aGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtg > 1:2813062/1‑70 (MQ=255) aGTAATATCCCCAAGTGATTGTCATATTTTTCAGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt < 1:878483/70‑1 (MQ=255) gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg < 1:457075/45‑1 (MQ=255) gTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc < 1:1763288/71‑3 (MQ=255) tAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACtgc > 1:3296041/1‑68 (MQ=255) aaTATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCa > 1:3256388/1‑45 (MQ=255) atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagcg > 1:2705721/1‑56 (MQ=255) atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCaa > 1:2033974/1‑71 (MQ=255) atatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCa < 1:5022952/70‑1 (MQ=255) tatCCCCAGCTGATAGTCATATTCTTCTGCGaaat < 1:367818/35‑2 (MQ=255) tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCATAGTAGCGTAGCCAGGCTACCaa < 1:4347204/70‑1 (MQ=255) tatCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAt < 1:2615888/71‑1 (MQ=255) atCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAAtt > 1:2975719/1‑71 (MQ=255) tCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTg > 1:3338200/1‑71 (MQ=255) cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg > 1:4068552/1‑71 (MQ=255) cccAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTggg > 1:1531737/1‑71 (MQ=255) cAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCaga < 1:1632740/43‑1 (MQ=255) gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAgtagc > 1:1134345/1‑46 (MQ=255) gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa < 1:1272417/71‑1 (MQ=255) gCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTaa < 1:2839072/71‑1 (MQ=255) | CCAGCGCAGCCAGCGTCACAAACAGCACAGGCAGGGTCATGATAATCCCTGTACGGAAGTAATATCCCCAGCTGATAGTCATATTCTTCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAA > minE/2595334‑2595474 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |