| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,892 | G→A | 10.0% | Y327Y (TAC→TAT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,892 | 0 | G | A | 10.0% | 199.7 / 9.0 | 70 | Y327Y (TAC→TAT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (27/36); new base A (4/3); total (31/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
GAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCG > minE/311823‑311962 | gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg > 1:2362560/1‑70 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa < 1:3480806/71‑1 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGAGTGGTGTCGTa > 1:2278972/1‑71 (MQ=255) tgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATggg > 1:3992616/1‑71 (MQ=255) atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAggg > 1:3219919/4‑69 (MQ=12) atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAggg > 1:3060770/4‑69 (MQ=12) atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa < 1:1614961/68‑1 (MQ=16) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACTGCGTGGTGTCGTATGGGTa < 1:2824688/71‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTa < 1:3785131/71‑1 (MQ=255) tggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAggg > 1:132925/3‑68 (MQ=16) gCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa > 1:4444151/1‑68 (MQ=16) cTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAAc > 1:3778978/1‑70 (MQ=255) cTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACa < 1:5563301/71‑1 (MQ=255) ttCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa > 1:3547085/1‑71 (MQ=255) tCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAt > 1:4448835/1‑71 (MQ=255) tCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAt > 1:3574996/1‑71 (MQ=255) cGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa < 1:2911483/69‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:4342279/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:2827703/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:286767/71‑1 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt > 1:5557755/1‑70 (MQ=255) aCCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGAAAACAAtttt < 1:1681204/71‑1 (MQ=255) cAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCa < 1:3497376/71‑1 (MQ=255) aGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:3461784/51‑1 (MQ=255) gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa < 1:5143234/71‑1 (MQ=255) gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa < 1:1235295/71‑1 (MQ=255) cGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:2735792/1‑51 (MQ=255) cGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:3057517/1‑51 (MQ=255) ttttTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATaag < 1:2968612/51‑3 (MQ=9) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt < 1:2709341/63‑1 (MQ=255) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt < 1:742067/63‑1 (MQ=255) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtt < 1:3855210/62‑1 (MQ=255) ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:2905741/71‑1 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:4541384/1‑48 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:1164868/1‑48 (MQ=255) aaCCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:668516/43‑1 (MQ=255) aaCCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:834660/43‑1 (MQ=255) aaCCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACg > 1:3010050/1‑71 (MQ=255) ccACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:2750151/41‑1 (MQ=255) cACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:439659/40‑1 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:1360000/38‑1 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:1152826/38‑1 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTc > 1:3240829/1‑56 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag < 1:4687316/71‑1 (MQ=255) cTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTAGAGAACCTTCATTCCCTGCGGgaag < 1:1628984/71‑1 (MQ=255) ttCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:3032935/37‑1 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGACGTATgg > 1:2802816/1‑40 (MQ=255) gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:2875209/1‑36 (MQ=255) gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:1221363/1‑37 (MQ=255) gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:587779/1‑37 (MQ=255) tAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTcc < 1:1808024/71‑1 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCAt > 1:743894/1‑71 (MQ=255) gaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCt < 1:5619689/71‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:3998361/47‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:4379600/47‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:22871/47‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:1177609/47‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAccc < 1:3293815/47‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGCAAACAATTTTCAGAccc < 1:5313097/47‑1 (MQ=255) ttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa > 1:4099146/1‑42 (MQ=255) ttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa > 1:5069204/1‑42 (MQ=255) aCGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:5685646/35‑1 (MQ=255) aCGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:3068489/35‑1 (MQ=255) aCGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:4524394/35‑1 (MQ=255) aCGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAg < 1:1618606/71‑1 (MQ=255) gAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGtt < 1:91683/71‑1 (MQ=255) gAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGtt > 1:1724388/1‑71 (MQ=255) gAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGtt < 1:1909821/71‑1 (MQ=255) aCGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGc > 1:4850607/1‑64 (MQ=255) cGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAg < 1:84959/71‑1 (MQ=255) gCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAAc > 1:1707413/1‑47 (MQ=255) gCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAAc > 1:4015709/1‑47 (MQ=255) gtggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTc > 1:2605170/1‑50 (MQ=255) gtgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGt > 1:3636944/1‑60 (MQ=255) gtgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGAt < 1:2725965/71‑1 (MQ=255) tgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGAtt < 1:4266013/71‑1 (MQ=255) tgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGAtt < 1:2673216/71‑1 (MQ=255) gtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCtt > 1:1919033/1‑51 (MQ=255) tCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGc < 1:172392/71‑1 (MQ=255) gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCAc > 1:713368/1‑62 (MQ=255) gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCg < 1:2286793/71‑1 (MQ=255) gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCg < 1:489665/71‑1 (MQ=255) | GAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCG > minE/311823‑311962 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |