| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,491 | G→A | 25.5% | C369C (TGC→TGT) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,491 | 0 | G | A | 25.5% | 257.1 / 91.7 | 153 | C369C (TGC→TGT) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (51/62); new base A (25/14); total (77/76) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-02 | |||||||||||
Only 100 of 158 total aligned reads displayed.
TCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAC > minE/2595421‑2595560 | tctGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCg > 1:855357/1‑71 (MQ=255) gCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAg > 1:2687148/1‑71 (MQ=255) cGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGc < 1:2793191/68‑1 (MQ=255) gAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGc > 1:3121949/1‑70 (MQ=255) aaaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCa < 1:1807966/71‑1 (MQ=255) aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa < 1:3251813/71‑1 (MQ=255) aaGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCaa < 1:4062936/71‑1 (MQ=255) aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt > 1:3615097/1‑71 (MQ=255) aGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAAt > 1:4120768/1‑71 (MQ=255) tACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca < 1:2745415/71‑1 (MQ=255) acatGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca > 1:1514912/4‑70 (MQ=255) gTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAt < 1:4778729/71‑1 (MQ=255) atGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt < 1:3814260/70‑1 (MQ=255) tGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACAt > 1:665170/1‑70 (MQ=255) gCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTg > 1:528571/1‑71 (MQ=255) gCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACAtt < 1:3803179/67‑1 (MQ=255) cAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa < 1:1972875/71‑1 (MQ=255) agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaa < 1:4839785/71‑1 (MQ=255) agagTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa > 1:2716251/1‑70 (MQ=255) agTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc < 1:5198531/71‑1 (MQ=255) gtagcgtagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc > 1:5664621/1‑70 (MQ=255) ttgcCAGACTGCCAACCGGGGTGATTTTCGGGCCTAAATCACAGCCAATcac < 1:5169098/50‑1 (MQ=255) tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac < 1:3148925/52‑1 (MQ=255) tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcac < 1:3109399/52‑1 (MQ=255) tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCAt < 1:5261264/67‑1 (MQ=255) tagcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCAt < 1:2479251/67‑1 (MQ=255) agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGct > 1:117541/1‑70 (MQ=255) agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAACCGCAGCCAATCACATTGGCATAAACCATCGctt > 1:1749143/1‑71 (MQ=255) gcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa < 1:4551350/59‑1 (MQ=255) gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTg > 1:830136/1‑54 (MQ=255) ccAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc < 1:1278617/62‑1 (MQ=255) cAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaaa > 1:725078/1‑59 (MQ=255) aGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt < 1:1136169/71‑1 (MQ=255) gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa > 1:3076353/1‑71 (MQ=255) gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa > 1:44154/1‑71 (MQ=255) ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct > 1:5456389/4‑66 (MQ=255) cTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATca < 1:2849464/43‑1 (MQ=255) tgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATca < 1:5408309/40‑1 (MQ=16) tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt > 1:2367820/1‑70 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATc < 1:4385994/57‑1 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc > 1:3451052/1‑71 (MQ=255) cAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATcaca < 1:2562623/41‑1 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCa > 1:1377362/1‑53 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:2905130/71‑1 (MQ=255) atcggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:97747/67‑1 (MQ=255) ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAcc < 1:5342575/50‑1 (MQ=255) ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:3748577/1‑69 (MQ=255) tcggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCa > 1:2381800/3‑51 (MQ=255) tGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:4094631/1‑68 (MQ=255) ggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgt > 1:1923256/1‑70 (MQ=255) ggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg < 1:3745095/70‑1 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATaaa > 1:1519117/1‑46 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAAc < 1:4203771/47‑1 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGcttctt > 1:2215367/1‑58 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa > 1:1869377/1‑64 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa > 1:623043/1‑64 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:4763680/1‑67 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:4738066/1‑67 (MQ=255) gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc > 1:3301054/1‑69 (MQ=255) ggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt < 1:1616517/60‑1 (MQ=255) gTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg < 1:4444327/57‑1 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:4639318/1‑71 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc < 1:848510/71‑1 (MQ=255) aaTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATa < 1:5461221/39‑1 (MQ=255) aTTTTCGGTCCCAAATCTCAGCCAATCACATTGGCATAAACAATCTATTCTTTGATAACGCCAGATGcc > 1:5660729/1‑69 (MQ=255) aTTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:3192559/61‑1 (MQ=255) ttttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt < 1:2214288/70‑1 (MQ=255) ttttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt < 1:3674096/70‑1 (MQ=255) ttttCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta > 1:3302211/1‑70 (MQ=255) tttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATa < 1:1283530/55‑1 (MQ=255) tttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:2993080/1‑71 (MQ=255) tttCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtac > 1:4252284/1‑69 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt < 1:1582934/46‑1 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGcc > 1:1162773/1‑55 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt > 1:5619693/1‑71 (MQ=255) cctaaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTc < 1:2033291/68‑1 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:50638/1‑60 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:3994363/1‑60 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:3866857/1‑60 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt > 1:763433/1‑70 (MQ=255) ctaaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTc > 1:2891717/3‑70 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt < 1:5252944/43‑1 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:3367170/70‑1 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:5299032/70‑1 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:3949922/70‑1 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:1722350/70‑1 (MQ=255) taaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa > 1:3565030/2‑71 (MQ=255) cAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa < 1:3422265/71‑1 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:1087065/1‑61 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt < 1:4455755/71‑1 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt > 1:2927406/1‑71 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt > 1:4870513/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg < 1:2150286/57‑1 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg < 1:131323/57‑1 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg < 1:334155/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc < 1:5437133/71‑2 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc < 1:911476/71‑2 (MQ=255) tCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt < 1:138087/41‑1 (MQ=255) gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc < 1:871666/70‑1 (MQ=255) gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc < 1:3386210/70‑1 (MQ=255) | TCTGCGAAAGTACGTGCAGCCAGAGTAGCGTAGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAC > minE/2595421‑2595560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |