| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,518 | C→T | 28.2% | A360A (GCG→GCA) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,518 | 0 | C | T | 28.2% | 299.9 / 137.6 | 199 | A360A (GCG→GCA) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base C (79/63); new base T (42/14); total (122/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.49e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01 | |||||||||||
Only 100 of 200 total aligned reads displayed.
AGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCG > minE/2595452‑2595582 | agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGct > 1:117541/1‑70 (MQ=255)agcCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAACCGCAGCCAATCACATTGGCATAAACCATCGctt > 1:1749143/1‑71 (MQ=255) gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctc > 1:4111743/1‑71 (MQ=255) gcCAGACTGCCAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctc > 1:138197/1‑71 (MQ=255) aGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt < 1:1136169/71‑1 (MQ=255) gCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGa > 1:44154/1‑71 (MQ=255) ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct > 1:3569147/4‑66 (MQ=255) ctgccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGcctct > 1:445603/4‑66 (MQ=255) ccAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAAc > 1:3451052/1‑71 (MQ=255) ccAATCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGacg < 1:530882/69‑3 (MQ=255) aaTCGGGGTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:2905130/71‑1 (MQ=255) atcggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:97747/67‑1 (MQ=255) ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:3177689/1‑69 (MQ=255) ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:3748577/1‑69 (MQ=255) ggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgt > 1:1923256/1‑70 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGcttctt > 1:2215367/1‑58 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa > 1:2880212/1‑64 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa > 1:623043/1‑64 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:4738066/1‑67 (MQ=255) gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc > 1:3301054/1‑69 (MQ=255) gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc > 1:5121328/1‑69 (MQ=255) gggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGa < 1:720008/70‑1 (MQ=255) ggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt < 1:1616517/60‑1 (MQ=255) gTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTg < 1:4444327/57‑1 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:4639318/1‑71 (MQ=255) aaTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg > 1:1316500/1‑71 (MQ=255) ttttCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGta > 1:870299/1‑70 (MQ=255) tttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATa < 1:1283530/55‑1 (MQ=255) tttCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtac > 1:4252284/1‑69 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt > 1:2205122/1‑46 (MQ=255) gTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt < 1:1582934/46‑1 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt > 1:5619693/1‑71 (MQ=255) cctaaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTc < 1:2033291/68‑1 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:3994363/1‑60 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:5393428/1‑60 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:50638/1‑60 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt > 1:3396011/1‑70 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGcttct < 1:3713674/41‑1 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCttt < 1:5252944/43‑1 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:3367170/70‑1 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:3949922/70‑1 (MQ=255) cAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa < 1:3422265/71‑1 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt > 1:2927406/1‑71 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg < 1:334155/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc > 1:269945/1‑51 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc < 1:911476/71‑2 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc < 1:5437133/71‑2 (MQ=255) tcacAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:2392320/4‑71 (MQ=255) tCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:4288879/1‑71 (MQ=255) tCGCAGCCAATCACATTGGCATAAACAATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:167266/1‑71 (MQ=255) cacAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAg > 1:5465245/3‑48 (MQ=255) gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc < 1:3255432/70‑1 (MQ=255) gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc < 1:4587681/70‑1 (MQ=255) acAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGAc < 1:1526969/69‑1 (MQ=255) acAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACa < 1:214923/70‑1 (MQ=255) cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt < 1:84031/65‑1 (MQ=255) aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt < 1:4159363/52‑1 (MQ=255) aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:595489/1‑62 (MQ=255) aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:2777726/1‑62 (MQ=255) aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:2176540/1‑62 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGcc < 1:553610/38‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc < 1:2079645/71‑1 (MQ=255) tCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:2594187/1‑61 (MQ=255) tCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:4010419/1‑61 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGa > 1:2980187/1‑40 (MQ=255) cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa > 1:5013667/1‑71 (MQ=255) acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca > 1:3832121/1‑65 (MQ=255) acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca > 1:223661/1‑65 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa > 1:3466013/1‑70 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa < 1:466127/70‑1 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:1037860/71‑1 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc > 1:2308503/1‑71 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:1966156/70‑1 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:823567/1‑57 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa < 1:4849085/71‑1 (MQ=255) ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc > 1:2718459/1‑44 (MQ=255) ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa > 1:79368/1‑52 (MQ=255) ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt > 1:2988091/1‑53 (MQ=255) ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt > 1:3608310/1‑53 (MQ=255) ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:3909527/71‑1 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa > 1:2678707/1‑51 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:2541286/1‑55 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:1689067/1‑55 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa > 1:3055542/1‑66 (MQ=255) ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa > 1:3308327/1‑50 (MQ=255) ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa > 1:3142306/1‑50 (MQ=255) ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt < 1:1822723/70‑1 (MQ=255) gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa < 1:2396974/70‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGt < 1:1312794/35‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGt < 1:2900094/35‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg < 1:135212/48‑1 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc > 1:1249439/1‑67 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc > 1:1088319/1‑67 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa > 1:2762815/1‑51 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:190643/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:4762948/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:4005558/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGAGCTGCCATCAATGGACAACGCGCCAAcc > 1:4243777/1‑61 (MQ=255) aaCCATAGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg > 1:3215243/1‑55 (MQ=255) cATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:769114/39‑1 (MQ=255) | AGCCAGGCTACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCG > minE/2595452‑2595582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |