| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,529 | T→C | 27.5% | I357V (ATC→GTC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,529 | 0 | T | C | 27.5% | 280.8 / 130.4 | 189 | I357V (ATC→GTC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base T (80/57); new base C (37/15); total (117/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01 | |||||||||||
Only 100 of 195 total aligned reads displayed.
TACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAA > minE/2595460‑2595597 | tACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt > 1:2367820/1‑70 (MQ=255) atcggggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGc < 1:97747/67‑1 (MQ=255) ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:3748577/1‑69 (MQ=255) ttGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGc > 1:3177689/1‑69 (MQ=255) ggggTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATaa > 1:1869377/1‑64 (MQ=255) gggTGATTTTCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc > 1:3301054/1‑69 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:4639318/1‑71 (MQ=255) tAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc < 1:848510/71‑1 (MQ=255) aaTTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCg > 1:1316500/1‑71 (MQ=255) aTTTTCGGTCCCAAATCTCAGCCAATCACATTGGCATAAACAATCTATTCTTTGATAACGCCAGATGcc > 1:5660729/1‑69 (MQ=255) ttttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt < 1:2214288/70‑1 (MQ=255) ttttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt < 1:3674096/70‑1 (MQ=255) tttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGACAGATGCCGtg < 1:1286167/70‑1 (MQ=255) tttCGGGCCTAAATCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtac > 1:4252284/1‑69 (MQ=255) ttCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct > 1:1945590/1‑71 (MQ=255) tCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgct > 1:803359/1‑70 (MQ=255) tCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt > 1:5619693/1‑71 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:5393428/1‑60 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:50638/1‑60 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:3994363/1‑60 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGcc > 1:3866857/1‑60 (MQ=255) cccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCAt > 1:763433/1‑70 (MQ=255) ctaaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTc > 1:2891717/3‑70 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:3949922/70‑1 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:1722350/70‑1 (MQ=255) ccAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:5299032/70‑1 (MQ=255) taaaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCaa > 1:3565030/2‑71 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:1087065/1‑61 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt > 1:2927406/1‑71 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt < 1:833011/71‑1 (MQ=255) aaaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAAt < 1:4455755/71‑1 (MQ=255) aaTCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg < 1:334155/71‑1 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAgtc > 1:269945/1‑51 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc < 1:5437133/71‑2 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAtc < 1:911476/71‑2 (MQ=255) aaTCACAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt > 1:2903195/1‑70 (MQ=255) tCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:4288879/1‑71 (MQ=255) cGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGAt < 1:1812747/40‑1 (MQ=255) gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc < 1:3386210/70‑1 (MQ=255) gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc < 1:871666/70‑1 (MQ=255) gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc < 1:4587681/70‑1 (MQ=255) gCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGAc < 1:3255432/70‑1 (MQ=255) acAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGAc < 1:1526969/69‑1 (MQ=255) acAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACa < 1:214923/70‑1 (MQ=255) cAGCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACag < 1:1179477/71‑2 (MQ=255) cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa < 1:770904/71‑1 (MQ=255) aGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGt < 1:2764875/52‑1 (MQ=255) gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg < 1:2046393/71‑1 (MQ=255) gCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcg < 1:2923227/71‑1 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:1266942/1‑52 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGtgc > 1:416460/1‑52 (MQ=255) ccAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc > 1:4701257/1‑71 (MQ=255) aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:1608917/1‑62 (MQ=255) aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:2176540/1‑62 (MQ=255) aTCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:66522/1‑62 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc < 1:5071896/71‑1 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc < 1:2079645/71‑1 (MQ=255) tCACATTGGCATAAATCATTGCCTCTTTGACGCCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCAcc > 1:2467144/1‑70 (MQ=255) tCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:4010419/1‑61 (MQ=255) tCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGa > 1:2594187/1‑61 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGa > 1:3407748/1‑40 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGa > 1:2980187/1‑40 (MQ=255) tCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCa < 1:2781269/71‑1 (MQ=255) cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATgg > 1:5564043/1‑59 (MQ=255) cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa > 1:5013667/1‑71 (MQ=255) acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca > 1:223661/1‑65 (MQ=255) acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca > 1:3832121/1‑65 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa < 1:466127/70‑1 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:1037860/71‑1 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:5472608/71‑1 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc > 1:3459158/1‑71 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:1634087/71‑1 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:168534/1‑57 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGa > 1:5175147/1‑57 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa < 1:4849085/71‑1 (MQ=255) ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGtactc > 1:2718459/1‑44 (MQ=255) ttGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAAt > 1:2988091/1‑53 (MQ=255) ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:1979696/71‑1 (MQ=255) ttGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAg < 1:3909527/71‑1 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa > 1:2678707/1‑51 (MQ=255) ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa > 1:3308327/1‑50 (MQ=255) ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCaa > 1:3142306/1‑50 (MQ=255) gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa < 1:2396974/70‑1 (MQ=255) gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa < 1:3422995/70‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGt < 1:1312794/35‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATg < 1:135212/48‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:1124572/1‑71 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:2560031/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg > 1:611440/1‑55 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg > 1:3736814/1‑55 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:4762948/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:1911825/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:4005558/1‑71 (MQ=255) aaCCATAGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg > 1:3215243/1‑55 (MQ=255) cATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATc < 1:4131968/39‑1 (MQ=255) gcctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCatat > 1:300896/4‑70 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt > 1:285528/1‑71 (MQ=255) cctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg < 1:4234961/69‑1 (MQ=255) tttGATAACGCCAGATGCCGTGCTGCCATCAATGGACa > 1:2753541/1‑38 (MQ=255) ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:1388473/1‑43 (MQ=255) | TACCAATTGGGGTAATTTTCGGTCCCAAATCGCAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAA > minE/2595460‑2595597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |