| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,562 | A→G | 16.5% | L346L (TTG→CTG) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,562 | 0 | A | G | 16.5% | 269.3 / 41.3 | 121 | L346L (TTG→CTG) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base A (53/48); new base G (9/11); total (62/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
CAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGC > minE/2595492‑2595630 | cAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa < 1:770904/71‑1 (MQ=255) gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg < 1:2046393/71‑1 (MQ=255) gCCAATCACATTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGcg > 1:4631505/1‑71 (MQ=255) aTCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGcc < 1:5071896/71‑1 (MQ=255) tCACATTGGCATAAATCATTGCCTCTTTGACGCCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCAcc > 1:2467144/1‑70 (MQ=255) cacaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa > 1:464410/1‑71 (MQ=255) acaTTGGCATAAATCATTGCCTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAgcgca > 1:3832121/1‑65 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa < 1:466127/70‑1 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:1037860/71‑1 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:3254161/71‑1 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc > 1:3459158/1‑71 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:5472608/71‑1 (MQ=255) acaTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc > 1:2308503/1‑71 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAcc < 1:1634087/71‑1 (MQ=255) caTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAAc < 1:1966156/70‑1 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAc < 1:2983404/61‑1 (MQ=255) aTTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa < 1:4849085/71‑1 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa > 1:3920017/1‑66 (MQ=255) tGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCaa > 1:3055542/1‑66 (MQ=255) ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt < 1:1472548/70‑1 (MQ=255) ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt < 1:1822723/70‑1 (MQ=255) ggCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGt < 1:4262823/70‑1 (MQ=255) gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa < 1:3422995/70‑1 (MQ=255) gCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTa < 1:2396974/70‑1 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa > 1:5394825/1‑53 (MQ=255) tAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:1124572/1‑71 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc > 1:1249439/1‑67 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTAc > 1:1088319/1‑67 (MQ=255) aaaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:2560031/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACaa > 1:2762815/1‑51 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg > 1:5035245/1‑55 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg > 1:2718291/1‑55 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg > 1:611440/1‑55 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg > 1:3736814/1‑55 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc < 1:4005571/70‑1 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:4571849/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:190643/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:4762948/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:4005558/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCg > 1:1911825/1‑71 (MQ=255) aaCCATCGCTTCTTTGATAACGCCAGATGCCGAGCTGCCATCAATGGACAACGCGCCAAcc > 1:4243777/1‑61 (MQ=255) aaCCATAGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgcg > 1:3215243/1‑55 (MQ=255) gcctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCatat > 1:300896/4‑70 (MQ=255) gCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATAtt > 1:285528/1‑71 (MQ=255) cctcttTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg < 1:4234961/69‑1 (MQ=255) cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAAcgc < 1:5218190/43‑1 (MQ=255) cttTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt < 1:1743118/69‑1 (MQ=255) tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt < 1:1311838/69‑1 (MQ=255) ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:1388473/1‑43 (MQ=255) ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:2577139/1‑43 (MQ=255) ttGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACgcgc > 1:367765/1‑43 (MQ=255) tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGt > 1:389031/1‑55 (MQ=255) tGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCat < 1:278853/70‑1 (MQ=255) gATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCataa > 1:467426/1‑71 (MQ=255) aTAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGt > 1:5610185/1‑64 (MQ=255) cgaCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTc < 1:2852098/63‑1 (MQ=255) aCGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat < 1:5301797/65‑1 (MQ=255) aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATaga < 1:1441744/71‑1 (MQ=255) aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:5685742/70‑1 (MQ=255) aCGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:4980414/70‑1 (MQ=255) cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa < 1:3890566/71‑1 (MQ=255) gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa > 1:2004573/1‑41 (MQ=255) gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCa > 1:1560367/1‑41 (MQ=255) gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaag < 1:1003764/71‑1 (MQ=255) ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:1625058/71‑1 (MQ=255) gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:1026562/1‑42 (MQ=255) gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:3029323/1‑42 (MQ=255) gCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTc > 1:5313032/1‑42 (MQ=255) gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg > 1:3789575/1‑51 (MQ=255) gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGt > 1:2675742/1‑52 (MQ=255) ccGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGtt > 1:4158798/1‑52 (MQ=255) ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat < 1:1134433/55‑1 (MQ=255) ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat < 1:4648604/55‑1 (MQ=255) cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg > 1:4332174/1‑69 (MQ=255) tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCgg < 1:2187126/40‑1 (MQ=255) tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg < 1:2098620/71‑1 (MQ=255) ctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCat > 1:569487/1‑41 (MQ=255) acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg > 1:2566778/2‑71 (MQ=255) cgtCAATCGACAGCGCACCAATCAGTACCGTCGGCATATTGTTCat > 1:1992271/3‑46 (MQ=25) cgtCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCat > 1:566624/3‑46 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:2668579/39‑1 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:1539955/39‑1 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTg < 1:3412685/39‑1 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa < 1:4671686/71‑1 (MQ=255) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt > 1:606189/1‑71 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:1108406/1‑43 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:2090024/1‑43 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:1973001/1‑43 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCata > 1:2973408/1‑43 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag > 1:2789313/1‑52 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc < 1:4929070/71‑3 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc < 1:3490046/71‑3 (MQ=255) aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc > 1:3660895/1‑70 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:2407353/45‑1 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:3637119/45‑1 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATag < 1:3300775/45‑1 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt < 1:218872/61‑1 (MQ=255) gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt < 1:3523388/71‑1 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa > 1:1057806/1‑53 (MQ=255) cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc > 1:3578976/1‑71 (MQ=255) | CAGCCAATCACATTGGCATAAACCATCGCTTCTTTGATAACGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGC > minE/2595492‑2595630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |