| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,513 | T→C | 9.8% | Q120Q (CAA→CAG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,513 | 0 | T | C | 9.8% | 252.7 / 13.9 | 92 | Q120Q (CAA→CAG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (50/33); new base C (6/3); total (56/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
GATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAC > minE/312444‑312583 | gATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtctt > 1:5520150/1‑71 (MQ=255)gATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtctt > 1:2008611/1‑71 (MQ=255)gATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtct > 1:3463587/1‑70 (MQ=255) aTAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtctt < 1:3206255/70‑1 (MQ=255) tAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGc < 1:724190/71‑1 (MQ=255) aCGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCgg < 1:3627125/71‑1 (MQ=255) cGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCgg > 1:816479/1‑70 (MQ=255) gCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCgg > 1:83276/1‑69 (MQ=255) gCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAAAGCTCACCCCTTGCTGCTGAACTTCTTGCgg > 1:465464/1‑69 (MQ=255) aaCCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGc < 1:4396384/61‑1 (MQ=255) ccATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCgg > 1:3797976/1‑61 (MQ=255) cATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCa > 1:4944481/1‑71 (MQ=255) cATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCa > 1:4614853/1‑71 (MQ=255) aTCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCAt < 1:4390367/71‑1 (MQ=255) aTCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCAt > 1:1760925/1‑71 (MQ=255) aGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtctt > 1:4292374/1‑52 (MQ=255) aGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCg < 1:1136844/70‑1 (MQ=255) ggAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGcc < 1:1255017/71‑1 (MQ=255) gAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCa < 1:4812912/71‑1 (MQ=255) gctgctGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCaa < 1:395066/57‑1 (MQ=255) gctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACg > 1:1248655/1‑56 (MQ=255) ctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATc > 1:899414/1‑60 (MQ=255) ctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATc > 1:4102938/1‑60 (MQ=255) tgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGc > 1:2005831/1‑44 (MQ=255) tgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGc > 1:1138464/1‑44 (MQ=255) tgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGc > 1:4150828/1‑44 (MQ=255) tgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt > 1:3063409/1‑71 (MQ=255) tgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt > 1:563107/1‑71 (MQ=255) ggATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAg < 1:1328137/69‑1 (MQ=255) ggATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGtt > 1:2160592/1‑71 (MQ=255) gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTg < 1:2282503/41‑1 (MQ=255) gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTg < 1:4504649/41‑1 (MQ=255) gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTg < 1:2077533/41‑1 (MQ=255) gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTg < 1:2900383/41‑1 (MQ=255) gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGttt > 1:1003350/1‑71 (MQ=255) atgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTg < 1:168728/71‑1 (MQ=255) tgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGt < 1:2535880/71‑1 (MQ=255) gatTTCTCAACGCTCACCCCTTGCTGCTGAActtct > 1:155326/1‑36 (MQ=255) gatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGtt > 1:2136240/1‑71 (MQ=255) atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGgcagc < 1:949662/44‑1 (MQ=255) atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCt > 1:4356838/1‑60 (MQ=255) atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCt > 1:4721322/1‑60 (MQ=255) atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTc > 1:3648995/1‑71 (MQ=255) tttCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCt > 1:2938909/1‑71 (MQ=255) tctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTg > 1:1653928/1‑70 (MQ=255) tctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTg > 1:4675381/1‑70 (MQ=255) ttcAACACTGATCCCCTGCTGCTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCa > 1:2213787/2‑71 (MQ=255) ttcAACACTGATCCCCTGCTGCTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCa > 1:3473372/2‑71 (MQ=255) ctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc < 1:2942190/70‑1 (MQ=255) aaCGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCt > 1:5522174/1‑71 (MQ=255) aCGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCAt > 1:3613632/1‑44 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTg < 1:381890/70‑1 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:5650579/71‑1 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:4243671/71‑1 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:1460206/71‑1 (MQ=255) gCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGaa > 1:2302376/1‑71 (MQ=255) cTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt < 1:5464836/54‑1 (MQ=255) cTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:5181787/1‑71 (MQ=255) cTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:5188030/1‑71 (MQ=255) cTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:4415100/1‑71 (MQ=255) tCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAcc > 1:2304822/1‑71 (MQ=255) aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCt < 1:1199429/70‑1 (MQ=255) aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg > 1:124613/1‑71 (MQ=255) aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg > 1:444578/1‑71 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTg < 1:195482/52‑1 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTg < 1:1997438/52‑1 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcg > 1:5373523/1‑71 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcg > 1:3268437/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:1201616/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc < 1:1253011/71‑1 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:3131442/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcg > 1:630402/1‑70 (MQ=255) cTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCAcc > 1:2674422/1‑59 (MQ=255) ttGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGtt < 1:3360678/47‑1 (MQ=255) ttGCTGCTCAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:2550526/61‑1 (MQ=255) tgctgcTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTg < 1:238437/41‑1 (MQ=19) tgctgcTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTg < 1:914114/41‑1 (MQ=19) tgctgcTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGa > 1:2052532/1‑70 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt > 1:3854876/1‑44 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCt > 1:442104/1‑57 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGata > 1:3822746/1‑71 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGata > 1:5065724/1‑71 (MQ=255) gctgctGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGtt < 1:2431612/45‑1 (MQ=16) gctgctGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTc > 1:3609236/1‑50 (MQ=19) gctgctGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTc > 1:3360919/1‑50 (MQ=19) ctgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGatat > 1:3947693/1‑71 (MQ=255) gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt < 1:3670099/41‑1 (MQ=255) gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt < 1:4092937/41‑1 (MQ=255) gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt < 1:4430184/41‑1 (MQ=255) gctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc < 1:4098675/58‑1 (MQ=255) ctgAACTTCTTGCGGCAGCACCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGCACCTGCGCGATATCCg > 1:2945349/1‑71 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:448469/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:4642006/1‑70 (MQ=255) cttcttGCGGCTGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc < 1:277137/44‑1 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc < 1:3342115/44‑1 (MQ=255) ttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGa < 1:2553563/49‑1 (MQ=255) tcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:1774050/1‑50 (MQ=255) tcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc > 1:1260472/1‑50 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg < 1:4296053/69‑1 (MQ=255) ttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:5200020/1‑71 (MQ=255) | GATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAC > minE/312444‑312583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |