| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,602 | A→T | 36.6% | S332S (TCT→TCA) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,602 | 0 | A | T | 36.6% | 34.8 / 40.5 | 41 | S332S (TCT→TCA) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base A (17/9); new base T (5/10); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.97e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01 | |||||||||||
CGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTT > minE/2595532‑2595670 | cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa < 1:464555/71‑1 (MQ=255)cGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaa < 1:3890566/71‑1 (MQ=255) gCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaag < 1:1003764/71‑1 (MQ=255) ccAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:1625058/71‑1 (MQ=255) gATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagagag < 1:5283863/71‑1 (MQ=255) cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaatg > 1:4332174/1‑69 (MQ=255) tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg < 1:2098620/71‑1 (MQ=255) acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg > 1:2566778/2‑71 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa < 1:4671686/71‑1 (MQ=255) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt > 1:606189/1‑71 (MQ=255) aaTGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAagag > 1:2789313/1‑52 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc < 1:3490046/71‑3 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc < 1:4929070/71‑3 (MQ=255) aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc > 1:3660895/1‑70 (MQ=255) tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt < 1:218872/61‑1 (MQ=255) gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt < 1:3523388/71‑1 (MQ=255) gACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGaa > 1:1057806/1‑53 (MQ=255) cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc > 1:3578976/1‑71 (MQ=255) gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:4148179/70‑1 (MQ=255) gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:3499369/70‑1 (MQ=255) cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:2020014/1‑42 (MQ=255) cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:5598692/1‑42 (MQ=255) cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATagaaga > 1:800238/1‑42 (MQ=255) cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:1755250/1‑65 (MQ=255) accAATCAGCACCGTCGGCATATTGTTCATGACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGaac < 1:4954810/69‑3 (MQ=255) accAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg > 1:5332894/2‑71 (MQ=255) ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt > 1:1209201/1‑71 (MQ=255) cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc < 1:5586629/71‑1 (MQ=255) cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt < 1:4575829/70‑1 (MQ=255) cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTc > 1:5083682/1‑71 (MQ=255) aatcAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcg > 1:1122560/4‑71 (MQ=255) aaCCAGTACCGTCGGCATATTGTTCATAATagaaga < 1:579353/36‑1 (MQ=255) cAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCgg > 1:4912015/1‑71 (MQ=255) aCCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc < 1:3247073/70‑1 (MQ=255) ccGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCca < 1:2492122/70‑1 (MQ=255) cGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata < 1:86341/71‑3 (MQ=255) gTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata > 1:1935347/1‑68 (MQ=255) gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg < 1:1573231/71‑1 (MQ=255) tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcataa > 1:4628111/1‑67 (MQ=255) cGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa < 1:1006725/42‑1 (MQ=255) ggCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGt < 1:836104/56‑1 (MQ=255) ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc > 1:1587922/1‑71 (MQ=255) gCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcat < 1:3142045/65‑2 (MQ=255) tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc > 1:2083290/1‑60 (MQ=255) ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:3211892/1‑40 (MQ=255) ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:5231212/1‑40 (MQ=255) ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:5356225/1‑40 (MQ=255) ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc > 1:4249838/1‑71 (MQ=255) ttCATAATAGAAGAGGGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTa < 1:2715517/66‑1 (MQ=255) aataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCa > 1:1830576/1‑71 (MQ=255) ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCAc > 1:1467928/1‑71 (MQ=255) aagaGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGtt < 1:4530913/70‑1 (MQ=255) | CGCCAGATGCCGTGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTT > minE/2595532‑2595670 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |