Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,595,614 G→C 37.2% T328T (ACC→ACG arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,595,6140GC37.2% 32.1 / 38.9 43T328T (ACC→ACGarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (17/10);  new base C (4/12);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 2.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAA  >  minE/2595544‑2595684
                                                                      |                                                                      
tgctgcCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCg                                                                        <  1:2098620/71‑1 (MQ=255)
     acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg                                                                   >  1:2566778/2‑71 (MQ=255)
        tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                                                                <  1:4671686/71‑1 (MQ=255)
         cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt                                                               >  1:606189/1‑71 (MQ=255)
          aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                                                              <  1:3490046/71‑3 (MQ=255)
          aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc                                                              <  1:4929070/71‑3 (MQ=255)
           aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc                                                              >  1:3660895/1‑70 (MQ=255)
            tGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGt                                                                      <  1:218872/61‑1 (MQ=255)
              gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt                                                          <  1:3523388/71‑1 (MQ=255)
                cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc                                                        >  1:3578976/1‑71 (MQ=255)
                  gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg                                                      <  1:4148179/70‑1 (MQ=255)
                  gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg                                                      <  1:3499369/70‑1 (MQ=255)
                   cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                                           >  1:1755250/1‑65 (MQ=255)
                      accAATCAGCACCGTCGGCATATTGTTCATGACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGaac                                                   <  1:4954810/69‑3 (MQ=255)
                      accAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg                                                  >  1:5332894/2‑71 (MQ=255)
                       ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt                                                 >  1:1209201/1‑71 (MQ=255)
                        cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc                                                <  1:5586629/71‑1 (MQ=255)
                        cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt                                                 <  1:4575829/70‑1 (MQ=255)
                        cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTc                                                >  1:5083682/1‑71 (MQ=255)
                         aatcAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcg                                               >  1:1122560/4‑71 (MQ=255)
                            cAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCgg                                            >  1:4912015/1‑71 (MQ=255)
                                aCCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc                                         <  1:3247073/70‑1 (MQ=255)
                                 ccGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCca                                        <  1:2492122/70‑1 (MQ=255)
                                  cGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata                                      <  1:86341/71‑3 (MQ=255)
                                   gTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata                                      >  1:1935347/1‑68 (MQ=255)
                                   gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg                                     <  1:1573231/71‑1 (MQ=255)
                                    tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcataa                                     >  1:4628111/1‑67 (MQ=255)
                                     cGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa                                                                <  1:1006725/42‑1 (MQ=255)
                                      ggCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGt                                                 <  1:836104/56‑1 (MQ=255)
                                      ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc                                  >  1:1587922/1‑71 (MQ=255)
                                       gCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcat                                       <  1:3142045/65‑2 (MQ=255)
                                          tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc                                         >  1:2083290/1‑60 (MQ=255)
                                            ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                                           >  1:3211892/1‑40 (MQ=255)
                                            ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                                           >  1:5231212/1‑40 (MQ=255)
                                            ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg                                                           >  1:5356225/1‑40 (MQ=255)
                                            ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc                            >  1:4249838/1‑71 (MQ=255)
                                               ttCATAATAGAAGAGGGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTa                              <  1:2715517/66‑1 (MQ=255)
                                                    aataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCa                    >  1:1830576/1‑71 (MQ=255)
                                                     ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCAc                   >  1:1467928/1‑71 (MQ=255)
                                                         aagaGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGtt                <  1:4530913/70‑1 (MQ=255)
                                                          tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa                    <  1:4123460/64‑1 (MQ=255)
                                                          tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa                    <  1:580369/64‑1 (MQ=255)
                                                           gagagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAg             <  1:1046953/71‑1 (MQ=255)
                                                             gagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTa           >  1:993494/1‑71 (MQ=255)
                                                                      ggTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa  <  1:2801304/71‑1 (MQ=255)
                                                                      |                                                                      
TGCTGCCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAA  >  minE/2595544‑2595684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: