| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,617 | G→C | 35.6% | L327L (CTC→CTG) ‡ | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,617 | 0 | G | C | 35.6% | 38.2 / 39.2 | 45 | L327L (CTC→CTG) ‡ | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (17/12); new base C (4/12); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.95e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
CCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAG > minE/2595549‑2595685 | acATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAAAAGAAGAGAGGAAGGCGGTGAg > 1:2566778/2‑71 (MQ=255) tCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa < 1:4671686/71‑1 (MQ=255) cAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAAt > 1:606189/1‑71 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc < 1:4929070/71‑3 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGaagc < 1:3490046/71‑3 (MQ=255) aTGGACAACCCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATc > 1:3660895/1‑70 (MQ=255) gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt < 1:3523388/71‑1 (MQ=255) cAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGc > 1:3578976/1‑71 (MQ=255) gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:4148179/70‑1 (MQ=255) gcgcaCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:3499369/70‑1 (MQ=255) cgcgCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:1755250/1‑65 (MQ=255) accAATCAGCACCGTCGGCATATTGTTCATGACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGaac < 1:4954810/69‑3 (MQ=255) accAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg > 1:5332894/2‑71 (MQ=255) ccAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt > 1:1209201/1‑71 (MQ=255) cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc < 1:5586629/71‑1 (MQ=255) cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGt < 1:4575829/70‑1 (MQ=255) cAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTc > 1:5083682/1‑71 (MQ=255) aatcAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTcg > 1:1122560/4‑71 (MQ=255) cAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCgg > 1:4912015/1‑71 (MQ=255) aCCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc < 1:3247073/70‑1 (MQ=255) ccGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCca < 1:2492122/70‑1 (MQ=255) cGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata < 1:86341/71‑3 (MQ=255) gTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcata > 1:1935347/1‑68 (MQ=255) gTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAg < 1:1573231/71‑1 (MQ=255) tCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcataa > 1:4628111/1‑67 (MQ=255) cGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGaa < 1:1006725/42‑1 (MQ=255) ggCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGt < 1:836104/56‑1 (MQ=255) ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc > 1:1587922/1‑71 (MQ=255) gCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCcat < 1:3142045/65‑2 (MQ=255) tatTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGccc > 1:2083290/1‑60 (MQ=255) ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:3211892/1‑40 (MQ=255) ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:5231212/1‑40 (MQ=255) ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCgg > 1:5356225/1‑40 (MQ=255) ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc > 1:4249838/1‑71 (MQ=255) ttCATAATAGAAGAGGGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTa < 1:2715517/66‑1 (MQ=255) aataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCa > 1:1830576/1‑71 (MQ=255) ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCAc > 1:1467928/1‑71 (MQ=255) aagaGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGtt < 1:4530913/70‑1 (MQ=255) tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa < 1:4123460/64‑1 (MQ=255) tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa < 1:580369/64‑1 (MQ=255) gagagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAg < 1:1046953/71‑1 (MQ=255) gagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTa > 1:993494/1‑71 (MQ=255) ggTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa < 1:2801304/71‑1 (MQ=255) gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg < 1:3897867/71‑1 (MQ=255) gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg < 1:3026881/71‑1 (MQ=255) | CCATCAATGGACAACGCGCCAACCAGTACCGTCGGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAG > minE/2595549‑2595685 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |