| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,650 | G→C | 8.5% | G316G (GGC→GGG) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,650 | 0 | G | C | 8.5% | 131.9 / 3.2 | 47 | G316G (GGC→GGG) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (24/19); new base C (2/2); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
GGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCA > minE/2595582‑2595716 | ggCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGcc > 1:1587922/1‑71 (MQ=255) ttGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc > 1:4249838/1‑71 (MQ=255) ttCATAATAGAAGAGGGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTa < 1:2715517/66‑1 (MQ=255) aataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCa > 1:1830576/1‑71 (MQ=255) ataGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCAc > 1:1467928/1‑71 (MQ=255) aagaGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGtt < 1:4530913/70‑1 (MQ=255) tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa < 1:580369/64‑1 (MQ=255) tgagagaAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCa < 1:4123460/64‑1 (MQ=255) gagagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAg < 1:1046953/71‑1 (MQ=255) gagGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTa > 1:993494/1‑71 (MQ=255) ggTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa < 1:2801304/71‑1 (MQ=255) gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg < 1:3026881/71‑1 (MQ=255) gTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAg < 1:3897867/71‑1 (MQ=255) aagAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCAGATTCAGCACGCCAGACAGgta > 1:3969711/3‑71 (MQ=255) aagAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGTCTGCCAGCAGATTCAGCACGCCAGACAGgta > 1:373104/3‑71 (MQ=255) aGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACg < 1:4723700/51‑1 (MQ=255) aaccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc < 1:3970417/69‑1 (MQ=255) aTCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTcc < 1:2700788/71‑1 (MQ=255) tCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa > 1:4175024/1‑62 (MQ=255) tCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGata > 1:4961856/1‑66 (MQ=255) ccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc > 1:3994860/1‑35 (MQ=255) ccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc > 1:3724830/1‑35 (MQ=255) ccGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATc > 1:3653529/1‑35 (MQ=255) gTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa < 1:153207/71‑1 (MQ=255) ccGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATcc < 1:5288169/71‑1 (MQ=255) tCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg < 1:2943725/66‑1 (MQ=255) gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:3402723/1‑71 (MQ=255) gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:981857/1‑71 (MQ=255) gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:1999490/1‑71 (MQ=255) cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGaaa < 1:3236981/45‑1 (MQ=255) cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa < 1:34383/57‑1 (MQ=255) cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa < 1:2347561/57‑1 (MQ=255) cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcgc < 1:3485629/71‑1 (MQ=255) ggCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:3567205/1‑69 (MQ=255) gCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCAtt < 1:2292966/65‑1 (MQ=255) ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGatat < 1:1465895/46‑1 (MQ=255) ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgc > 1:3961938/1‑65 (MQ=255) ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc > 1:2526325/1‑71 (MQ=255) ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc > 1:5602778/1‑71 (MQ=255) ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc > 1:3780043/1‑71 (MQ=255) cacaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc > 1:4903128/1‑70 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa < 1:5096946/52‑1 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:4126379/1‑71 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:2682705/1‑71 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:1896900/1‑71 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:4029242/1‑71 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:2875794/1‑71 (MQ=255) | GGCATATTGTTCATAATAGAAGAGAGGAAGGCGGTGAGGAATCCGGTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCA > minE/2595582‑2595716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |