| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,695 | T→G | 10.0% | L301F (TTA→TTC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,695 | 0 | T | G | 10.0% | 236.0 / 21.1 | 100 | L301F (TTA→TTC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base T (52/38); new base G (4/6); total (56/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGG > minE/2595627‑2595765 | gTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa < 1:153207/71‑1 (MQ=255) ccGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATcc < 1:5288169/71‑1 (MQ=255) tCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg < 1:2943725/66‑1 (MQ=255) gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:981857/1‑71 (MQ=255) gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:3402723/1‑71 (MQ=255) gcgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:1999490/1‑71 (MQ=255) cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa < 1:2347561/57‑1 (MQ=255) cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTa < 1:34383/57‑1 (MQ=255) cgGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcgc < 1:3485629/71‑1 (MQ=255) ggCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:3567205/1‑69 (MQ=255) gCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCAtt < 1:2292966/65‑1 (MQ=255) ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgc > 1:3961938/1‑65 (MQ=255) ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc > 1:2526325/1‑71 (MQ=255) ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc > 1:3780043/1‑71 (MQ=255) ccACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc > 1:5602778/1‑71 (MQ=255) cacaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGc > 1:4903128/1‑70 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTaa < 1:5096946/52‑1 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:1896900/1‑71 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:4029242/1‑71 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:4126379/1‑71 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:2875794/1‑71 (MQ=255) acaGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCa > 1:2682705/1‑71 (MQ=255) cGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc < 1:5526257/71‑1 (MQ=255) ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg < 1:5383454/49‑1 (MQ=255) ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg < 1:5618776/49‑1 (MQ=255) ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg < 1:127529/49‑1 (MQ=255) ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg < 1:2278032/49‑1 (MQ=255) ttATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa < 1:4828598/67‑1 (MQ=255) tATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac < 1:2842828/70‑1 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa > 1:1257627/1‑49 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCa > 1:4861178/1‑49 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAgg > 1:2411165/1‑58 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac > 1:2871876/1‑69 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac > 1:4305840/1‑69 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac > 1:1762316/1‑69 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca > 1:2537540/1‑68 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca > 1:1326877/1‑68 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacca > 1:5442086/1‑68 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc > 1:2400230/1‑67 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAacc > 1:4164990/1‑67 (MQ=255) aTCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac > 1:1798524/1‑66 (MQ=255) ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa > 1:5314827/3‑61 (MQ=255) ctgCCAGCAGATTCAGCACGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATa > 1:3648782/3‑61 (MQ=255) cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa < 1:5487768/70‑1 (MQ=255) cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa < 1:2578486/70‑1 (MQ=255) cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa < 1:237230/70‑1 (MQ=255) cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt < 1:394464/71‑1 (MQ=255) cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt < 1:4913772/71‑1 (MQ=255) ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAAt < 1:1728665/38‑1 (MQ=255) ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAAt < 1:4727417/38‑1 (MQ=255) ccAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATa < 1:5692575/70‑1 (MQ=255) cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATc > 1:2163570/1‑38 (MQ=255) cAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATc > 1:3740996/1‑38 (MQ=255) aGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACAt > 1:619326/1‑71 (MQ=255) cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg < 1:4507846/71‑1 (MQ=255) cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg < 1:2684351/71‑1 (MQ=255) gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg < 1:913053/35‑1 (MQ=255) gTTGAGTACGCCAGAAAGATATTCCGTTAATCCgg < 1:2980581/35‑1 (MQ=255) tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAAccacca > 1:1491589/1‑57 (MQ=255) tGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc > 1:1370406/1‑71 (MQ=255) gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:564529/1‑39 (MQ=255) gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTgcg > 1:53938/1‑39 (MQ=255) gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac > 1:5452169/1‑51 (MQ=255) gAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAac > 1:962069/1‑51 (MQ=255) aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa < 1:3268683/71‑1 (MQ=255) aCGCCAGAAAGATATTCCTTTAATCCAGCATTGCGCAGGCCATaaa > 1:4252219/1‑46 (MQ=255) aCGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaaa > 1:3645637/1‑46 (MQ=255) aCGACAGAAAGATATTCCGTTAATCCGGCATTGAGCAGGCCATaaa > 1:545180/1‑46 (MQ=39) cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa > 1:40940/1‑44 (MQ=255) cGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag > 1:3286343/1‑68 (MQ=255) gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa < 1:951590/71‑3 (MQ=255) ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt < 1:328670/71‑1 (MQ=255) ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac > 1:1359151/1‑47 (MQ=255) ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAaccac > 1:3714069/1‑47 (MQ=255) ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt < 1:5096957/71‑1 (MQ=255) ccAGAAAGATATTCCGTTAATCCGGAATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga > 1:4739359/1‑70 (MQ=255) cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt < 1:3390875/70‑1 (MQ=255) cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt > 1:4179180/1‑70 (MQ=255) aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa < 1:3853428/71‑1 (MQ=255) gAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATaa < 1:885608/39‑1 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa > 1:1902425/1‑64 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa > 1:3888093/1‑64 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa > 1:5593260/1‑64 (MQ=255) aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt > 1:5290268/1‑71 (MQ=255) atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag < 1:3366957/61‑1 (MQ=255) atTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt > 1:4202109/1‑61 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagc < 1:343597/52‑1 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc < 1:2489466/71‑1 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc < 1:5425102/71‑1 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:3432388/70‑1 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:639752/70‑1 (MQ=255) tCCGTTAATCCGGCATTGCGCAGGCCATAAaccacc > 1:1406758/1‑36 (MQ=255) tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:4215380/1‑69 (MQ=255) ccGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg > 1:3361955/1‑71 (MQ=255) ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGcc < 1:1361447/46‑1 (MQ=255) ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGc > 1:1502745/1‑68 (MQ=255) cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga > 1:3009797/1‑56 (MQ=255) cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa < 1:2437817/69‑1 (MQ=255) ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag < 1:5134430/50‑1 (MQ=255) tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:2040723/1‑71 (MQ=255) | GTGCCGAGCGTCGCGGCCCACAGGCCGTTATCCGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGG > minE/2595627‑2595765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |