| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,728 | A→G | 30.7% | Y290Y (TAT→TAC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,728 | 0 | A | G | 30.7% | 156.3 / 98.4 | 127 | Y290Y (TAT→TAC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base A (44/44); new base G (18/21); total (62/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
CGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAT > minE/2595659‑2595798 | cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa < 1:5487768/70‑1 (MQ=255)cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa < 1:2578486/70‑1 (MQ=255)cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGa < 1:237230/70‑1 (MQ=255)cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt < 1:4913772/71‑1 (MQ=255)cGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGAt < 1:394464/71‑1 (MQ=255) aGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACAt > 1:619326/1‑71 (MQ=255) cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg < 1:4507846/71‑1 (MQ=255) cGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcg < 1:2684351/71‑1 (MQ=255) aCGCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaa < 1:3268683/71‑1 (MQ=255) gCCAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGagaaaa < 1:951590/71‑3 (MQ=255) ccAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt < 1:328670/71‑1 (MQ=255) ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt < 1:5096957/71‑1 (MQ=255) ccAGAAAGATATTCCGTTAATCCGGAATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga > 1:4739359/1‑70 (MQ=255) cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt > 1:4179180/1‑70 (MQ=255) cAGACAGGTACTCCGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAAt < 1:3390875/70‑1 (MQ=255) aGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa < 1:3853428/71‑1 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa > 1:1902425/1‑64 (MQ=255) aaaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaa > 1:3888093/1‑64 (MQ=255) atatTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaag < 1:3366957/61‑1 (MQ=255) atTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGAt > 1:4202109/1‑61 (MQ=255) ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:3432388/70‑1 (MQ=255) tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:4215380/1‑69 (MQ=255) ccGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg > 1:3361955/1‑71 (MQ=255) ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGcc < 1:1361447/46‑1 (MQ=255) ccGTGAACCCTGCATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGc > 1:1502745/1‑68 (MQ=255) cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGagaaga > 1:3009797/1‑56 (MQ=255) cGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa < 1:2437817/69‑1 (MQ=255) ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCcgagcgag < 1:5134430/50‑1 (MQ=255) tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:2040723/1‑71 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:5190734/70‑1 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:1520409/70‑1 (MQ=255) tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc < 1:553044/69‑1 (MQ=255) tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc < 1:395201/69‑1 (MQ=255) tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc < 1:177354/69‑1 (MQ=255) cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCt < 1:4706119/57‑1 (MQ=255) gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc > 1:5226937/1‑54 (MQ=255) gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc > 1:1661599/1‑54 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc > 1:4682640/1‑53 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc > 1:5413957/1‑53 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc > 1:5480005/1‑53 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg > 1:311521/1‑70 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg > 1:1359999/1‑70 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg < 1:4448805/70‑1 (MQ=255) cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggg > 1:1810563/1‑61 (MQ=255) ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga < 1:5646928/40‑1 (MQ=255) ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga < 1:4334479/40‑1 (MQ=255) ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTgaga < 1:628327/40‑1 (MQ=255) ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCa > 1:1753101/1‑70 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg < 1:4337317/71‑1 (MQ=255) tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:4959400/1‑70 (MQ=255) tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:4774993/47‑1 (MQ=255) tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:4538676/47‑1 (MQ=255) tGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:1414141/47‑1 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGAt > 1:4017813/1‑48 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATc > 1:5028236/1‑49 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCAtggc > 1:4981360/1‑57 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCAtggc > 1:5300690/1‑57 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAgg < 1:1432804/70‑1 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGa > 1:2553886/1‑71 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa > 1:1478264/1‑47 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa > 1:5657266/1‑47 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc > 1:3537021/1‑49 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATc > 1:838677/1‑49 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa > 1:3144657/1‑71 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa > 1:5474520/1‑71 (MQ=255) aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt < 1:3163220/71‑1 (MQ=255) aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:5676939/70‑1 (MQ=255) ggCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:2847733/69‑1 (MQ=255) gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc > 1:3936944/1‑71 (MQ=255) ccATAAACCACCAGATACATGCCGAGCGAGAAGATGa > 1:1987143/1‑37 (MQ=255) ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:5705/39‑1 (MQ=255) ccATAAACCACCAGATACATGCCGAGCGAGAAGATGACa < 1:5257019/39‑1 (MQ=255) cATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg > 1:1163787/1‑48 (MQ=255) aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc < 1:4301593/63‑1 (MQ=255) aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc < 1:786349/63‑1 (MQ=255) aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc < 1:1097056/63‑1 (MQ=255) aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccgg < 1:906572/71‑3 (MQ=255) aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:3889169/43‑1 (MQ=255) aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:3435103/43‑1 (MQ=255) aTAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAg > 1:5338130/1‑46 (MQ=255) tAAACCACCAGATATATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:4049902/49‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc < 1:3462397/42‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc < 1:299542/69‑1 (MQ=255) aaaCCACCAGATACATGCCGAGCGAGAAGATGACAAt > 1:2089697/1‑37 (MQ=255) aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa > 1:4664768/1‑42 (MQ=255) aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa > 1:5545859/1‑42 (MQ=255) aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa > 1:1519448/1‑42 (MQ=255) aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc > 1:5229832/1‑51 (MQ=255) accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt < 1:719264/71‑1 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:945133/1‑38 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:1025090/1‑38 (MQ=255) ccaccaGATACATGCCGAGCGAGAAGATGACAATCTGc > 1:1349049/1‑38 (MQ=255) caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa < 1:257140/71‑1 (MQ=255) ccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATc < 1:2879642/71‑1 (MQ=255) caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg < 1:3722434/71‑1 (MQ=255) aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg > 1:1036405/1‑50 (MQ=255) aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg > 1:4186020/1‑70 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa > 1:2385229/1‑65 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc < 1:5510319/69‑1 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAt < 1:1668657/71‑1 (MQ=255) | CGCCAGCACGTTGAGTACGCCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAT > minE/2595659‑2595798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |