| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,595,764 | G→C | 35.5% | A278A (GCC→GCG) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,595,764 | 0 | G | C | 35.5% | 93.2 / 86.7 | 93 | A278A (GCC→GCG) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (33/27); new base C (17/16); total (50/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
TAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGC > minE/2595695‑2595832 | tAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg > 1:2040723/1‑71 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:5190734/70‑1 (MQ=255) aaTCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:1520409/70‑1 (MQ=255) tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc < 1:177354/69‑1 (MQ=255) tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc < 1:395201/69‑1 (MQ=255) tCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGc < 1:553044/69‑1 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg > 1:311521/1‑70 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg > 1:1359999/1‑70 (MQ=255) cATTGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACcgcg < 1:4448805/70‑1 (MQ=255) cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg > 1:5508891/1‑70 (MQ=255) cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg > 1:3923888/1‑70 (MQ=255) cATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgcg > 1:4283970/1‑70 (MQ=255) ttGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCa > 1:1753101/1‑70 (MQ=255) ttGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg < 1:4337317/71‑1 (MQ=255) tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:2682276/1‑70 (MQ=255) tGCGCAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAg > 1:4959400/1‑70 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAgg < 1:1432804/70‑1 (MQ=255) gcgcAGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGa > 1:2553886/1‑71 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa > 1:3144657/1‑71 (MQ=255) gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa > 1:5474520/1‑71 (MQ=255) cgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGAc < 1:3725958/71‑1 (MQ=255) cAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACtt > 1:117966/1‑71 (MQ=255) aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt < 1:3163220/71‑1 (MQ=255) aGGCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:5676939/70‑1 (MQ=255) ggCCATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtt < 1:2847733/69‑1 (MQ=255) gCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAc > 1:3936944/1‑71 (MQ=255) ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt > 1:3117455/1‑68 (MQ=255) ccATAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACtttt > 1:176605/1‑68 (MQ=255) aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc < 1:3387899/63‑1 (MQ=255) aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc < 1:1097056/63‑1 (MQ=255) aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc < 1:4301593/63‑1 (MQ=255) aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc < 1:786349/63‑1 (MQ=255) aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc < 1:192852/63‑1 (MQ=255) aTAGACCACGATGTACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccgg < 1:906572/71‑3 (MQ=255) tAAACCACCAGATATATGCCGAGCGAGAAGATGACAATCTGCCAggggg < 1:4049902/49‑1 (MQ=255) tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTAccc < 1:299542/69‑1 (MQ=255) aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc > 1:5229832/1‑51 (MQ=255) aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc > 1:1532783/1‑51 (MQ=255) accaccAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAtt < 1:719264/71‑1 (MQ=255) caccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa < 1:257140/71‑1 (MQ=255) ccaGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATc < 1:2879642/71‑1 (MQ=255) caGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg < 1:3722434/71‑1 (MQ=255) aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg > 1:234510/1‑50 (MQ=255) aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAg > 1:1036405/1‑50 (MQ=255) aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg > 1:4186020/1‑70 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa > 1:2385229/1‑65 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc < 1:5510319/69‑1 (MQ=255) aTACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCAt < 1:1668657/71‑1 (MQ=255) tACATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAAt < 1:1845789/70‑1 (MQ=255) aCATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGa > 1:5107652/1‑71 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGtg > 1:5689818/1‑57 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGtg > 1:4378702/1‑57 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc > 1:180481/1‑71 (MQ=255) cATGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAc > 1:1060550/1‑71 (MQ=255) aTGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGAc < 1:2724969/47‑1 (MQ=255) tGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa < 1:2975736/59‑1 (MQ=255) tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt < 1:4840439/49‑1 (MQ=255) tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACttt < 1:4282590/49‑1 (MQ=255) gCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa > 1:3895040/1‑44 (MQ=255) gCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa > 1:4394685/1‑44 (MQ=255) ccGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg < 1:2361138/71‑1 (MQ=255) cgagcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt < 1:4160915/71‑1 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt > 1:3451039/1‑69 (MQ=255) agcgagAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt > 1:1319482/1‑69 (MQ=255) agaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACtt > 1:3309099/1‑39 (MQ=19) agaaaaTAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccg > 1:1081720/1‑43 (MQ=255) gaagaTGACAATCTTCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:5332342/1‑65 (MQ=255) gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:2317579/1‑65 (MQ=255) gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt > 1:189617/1‑65 (MQ=255) gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcga > 1:713254/1‑71 (MQ=255) aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgc > 1:1017289/1‑61 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa < 1:4941705/45‑1 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGcgacga > 1:4151440/1‑70 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac < 1:4033741/71‑1 (MQ=255) aTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCgacgac < 1:1089310/71‑1 (MQ=255) tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa > 1:3044607/1‑53 (MQ=255) tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa > 1:2307392/1‑53 (MQ=255) tGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa < 1:694332/71‑1 (MQ=255) gACAATCTGCCAGGTGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc > 1:2381533/1‑63 (MQ=255) gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc > 1:2823252/1‑63 (MQ=255) gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc > 1:1600690/1‑63 (MQ=255) gACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACa > 1:1396365/1‑70 (MQ=255) aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa < 1:1027949/71‑1 (MQ=255) aCAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa < 1:4115735/71‑1 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa > 1:2115101/1‑70 (MQ=255) cAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaat < 1:3595832/71‑1 (MQ=255) aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa < 1:5520304/39‑1 (MQ=255) aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGt < 1:3955823/54‑1 (MQ=255) aTCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataa < 1:5658511/71‑1 (MQ=255) tCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaacag > 1:1109487/1‑67 (MQ=255) cTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACaaa > 1:3463228/1‑66 (MQ=255) ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCtttt > 1:334792/1‑51 (MQ=255) ccATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCTTTTCGCCACCACaaacag < 1:3102050/66‑4 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAaataat < 1:5305533/67‑1 (MQ=255) ccAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAatat < 1:5287046/69‑1 (MQ=255) cAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAg > 1:4824907/1‑71 (MQ=255) aGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGc > 1:939516/1‑37 (MQ=255) ggggCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAgcgc < 1:5058683/71‑1 (MQ=255) | TAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGCGACGACAAATAATATCAGCGC > minE/2595695‑2595832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |