| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,596,087 | C→T | 10.4% | G171S (GGC→AGC) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,596,087 | 0 | C | T | 10.4% | 231.4 / 13.6 | 86 | G171S (GGC→AGC) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base C (30/47); new base T (6/3); total (36/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01 | |||||||||||
TAACATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTA > minE/2596016‑2596154 | tAACATCACCAGCGT‑‑CAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGcc < 1:1656161/70‑1 (MQ=255) aCATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGcc > 1:5391526/1‑70 (MQ=255) aCATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGcc < 1:4792270/70‑1 (MQ=255) aCATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGcc < 1:4053632/70‑1 (MQ=255) aCATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGcc > 1:2167578/1‑70 (MQ=255) aCATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGcc > 1:169686/1‑70 (MQ=255) cATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGa < 1:95430/71‑1 (MQ=255) aTCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGa < 1:2708317/70‑1 (MQ=255) aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGc > 1:1023456/1‑70 (MQ=255) aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGc > 1:900574/1‑70 (MQ=255) aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGc > 1:3730694/1‑70 (MQ=255) aCCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGc > 1:1413762/1‑70 (MQ=255) gCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCaa < 1:1658216/69‑1 (MQ=255) gCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATc < 1:4271830/71‑1 (MQ=255) acaaTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGc > 1:4656980/1‑71 (MQ=255) aTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGa < 1:350982/71‑1 (MQ=255) gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGaaa < 1:4626850/70‑1 (MQ=255) gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGaaa < 1:4620175/70‑1 (MQ=255) ggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGa < 1:1529366/71‑1 (MQ=255) cgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata > 1:5208722/1‑71 (MQ=255) tatCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGa < 1:2569275/61‑1 (MQ=255) atCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCa > 1:3750295/1‑71 (MQ=255) tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata > 1:4246305/1‑66 (MQ=255) tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata > 1:4212338/1‑66 (MQ=255) tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata > 1:3468187/1‑66 (MQ=255) tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTc > 1:2542745/1‑69 (MQ=255) cACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGaa < 1:1592809/48‑1 (MQ=255) cACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAAt > 1:3275166/1‑50 (MQ=255) cACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAcc < 1:4238865/70‑1 (MQ=255) cACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa < 1:2734764/71‑1 (MQ=255) aCCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGaaa > 1:35870/1‑48 (MQ=255) ccGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGaa < 1:5404506/46‑1 (MQ=255) ccGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAAt > 1:1408805/1‑48 (MQ=255) ccGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGa < 1:245251/55‑1 (MQ=255) cGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGt < 1:3055166/71‑1 (MQ=255) ggCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGaaa > 1:878055/1‑55 (MQ=255) ggCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt < 1:2563728/71‑1 (MQ=255) gCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg < 1:3655638/71‑1 (MQ=255) gatcATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGt > 1:1748718/4‑68 (MQ=255) ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATc < 1:2693380/43‑1 (MQ=255) ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATc < 1:1598296/43‑1 (MQ=255) ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATc < 1:3048243/43‑1 (MQ=255) ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATc < 1:642444/43‑1 (MQ=255) ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc < 1:3571826/52‑1 (MQ=255) cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa > 1:3207204/1‑69 (MQ=255) cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa > 1:2358490/1‑69 (MQ=255) cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaa > 1:4646796/1‑69 (MQ=255) tCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc < 1:2312640/49‑1 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg > 1:467677/1‑63 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa > 1:2641538/1‑70 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt < 1:1330378/71‑1 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt < 1:249723/71‑1 (MQ=255) cGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAg < 1:2248939/38‑1 (MQ=255) cGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGa < 1:1179556/47‑1 (MQ=255) cGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt > 1:3443319/1‑69 (MQ=255) gCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg > 1:1004157/1‑71 (MQ=255) cGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGc < 1:4355585/71‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc < 1:2006210/38‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACg < 1:3508099/39‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACg < 1:3665311/39‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACg < 1:5605021/39‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg < 1:1461946/50‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg < 1:3006230/50‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGACATTCACCAg < 1:1082108/50‑1 (MQ=255) aTTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAgg > 1:349147/1‑50 (MQ=255) aTTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa < 1:1196447/62‑1 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc < 1:2061554/36‑1 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc < 1:223291/45‑1 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg > 1:2173524/1‑52 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg > 1:4434754/1‑52 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGAAATTCACAAGGTTGGAGACAATAAGCGGCAgg > 1:2493236/1‑70 (MQ=255) tCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc < 1:3576795/35‑1 (MQ=255) tCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata > 1:2091012/1‑39 (MQ=255) tCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg > 1:5291843/1‑62 (MQ=255) cgcg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc > 1:5292188/1‑43 (MQ=255) gcg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata < 1:1122080/37‑1 (MQ=255) cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg > 1:3387305/4‑71 (MQ=255) cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg > 1:3393996/4‑71 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc > 1:351590/1‑71 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc < 1:3513256/71‑1 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc < 1:1877612/71‑1 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc > 1:1196194/1‑71 (MQ=255) g‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt < 1:4460923/46‑1 (MQ=255) g‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt < 1:519162/46‑1 (MQ=255) g‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg < 1:3270281/71‑1 (MQ=255) caaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACa < 1:5531407/52‑1 (MQ=255) aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg < 1:3444343/60‑1 (MQ=255) aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg < 1:11626/60‑1 (MQ=255) aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTTGAGACAATAAGCGGCAGGCTGGCgg > 1:2759706/1‑71 (MQ=255) aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa < 1:5092252/39‑1 (MQ=255) aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTa > 1:4325507/1‑71 (MQ=255) gCCGAGGCCAAATAAATCAGCGGAAACGATATTCACCAGGtt < 1:4819783/42‑1 (MQ=255) | TAACATCACCAGCGTGGCAACAATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTA > minE/2596016‑2596154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |