| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,596,106 | G→C | 15.4% | S164S (TCC→TCG) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,596,106 | 0 | G | C | 15.4% | 232.3 / 31.3 | 97 | S164S (TCC→TCG) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (36/46); new base C (7/8); total (43/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
ATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGG > minE/2596038‑2596170 | aTCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGa < 1:350982/71‑1 (MQ=255) gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGaaa < 1:4626850/70‑1 (MQ=255) gcggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGaaa < 1:4620175/70‑1 (MQ=255) ggcgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGa < 1:1529366/71‑1 (MQ=255) cgATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata > 1:5208722/1‑71 (MQ=255) tatCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGa < 1:2569275/61‑1 (MQ=255) atCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCa > 1:3750295/1‑71 (MQ=255) tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata > 1:4212338/1‑66 (MQ=255) tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata > 1:4246305/1‑66 (MQ=255) tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata > 1:3468187/1‑66 (MQ=255) tCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTc > 1:2542745/1‑69 (MQ=255) cACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAcc < 1:4238865/70‑1 (MQ=255) cACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa < 1:2734764/71‑1 (MQ=255) ccGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGa < 1:245251/55‑1 (MQ=255) cGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGt < 1:3055166/71‑1 (MQ=255) ggCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGaaa > 1:878055/1‑55 (MQ=255) ggCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt < 1:2563728/71‑1 (MQ=255) gCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg < 1:3655638/71‑1 (MQ=255) gatcATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGt > 1:1748718/4‑68 (MQ=255) ccATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc < 1:3571826/52‑1 (MQ=255) cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa > 1:2358490/1‑69 (MQ=255) cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa > 1:3207204/1‑69 (MQ=255) cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaa > 1:4646796/1‑69 (MQ=255) tCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc < 1:2312640/49‑1 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg > 1:467677/1‑63 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa > 1:2641538/1‑70 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt < 1:1330378/71‑1 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt < 1:249723/71‑1 (MQ=255) cGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGa < 1:1179556/47‑1 (MQ=255) cGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt > 1:3443319/1‑69 (MQ=255) gCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg > 1:1004157/1‑71 (MQ=255) cGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGc < 1:4355585/71‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc < 1:2006210/38‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACg < 1:3665311/39‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACg < 1:5605021/39‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACg < 1:3508099/39‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg < 1:1461946/50‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAg < 1:3006230/50‑1 (MQ=255) tATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGACATTCACCAg < 1:1082108/50‑1 (MQ=255) aTTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAgg > 1:349147/1‑50 (MQ=255) aTTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa < 1:1196447/62‑1 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc < 1:2061554/36‑1 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc < 1:223291/45‑1 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg > 1:2173524/1‑52 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg > 1:4434754/1‑52 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGAAATTCACAAGGTTGGAGACAATAAGCGGCAgg > 1:2493236/1‑70 (MQ=255) tCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAAc < 1:3576795/35‑1 (MQ=255) tCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata > 1:2091012/1‑39 (MQ=255) tCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg > 1:5291843/1‑62 (MQ=255) cgcg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCAc > 1:5292188/1‑43 (MQ=255) gcg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGata < 1:1122080/37‑1 (MQ=255) cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg > 1:3387305/4‑71 (MQ=255) cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg > 1:3393996/4‑71 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc > 1:1196194/1‑71 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc < 1:1877612/71‑1 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc > 1:351590/1‑71 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc < 1:3513256/71‑1 (MQ=255) g‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt < 1:519162/46‑1 (MQ=255) g‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGtt < 1:4460923/46‑1 (MQ=255) g‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg < 1:3270281/71‑1 (MQ=255) caaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACa < 1:5531407/52‑1 (MQ=255) aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg < 1:3444343/60‑1 (MQ=255) aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg < 1:11626/60‑1 (MQ=255) aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTTGAGACAATAAGCGGCAGGCTGGCgg > 1:2759706/1‑71 (MQ=255) aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCa < 1:5092252/39‑1 (MQ=255) aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTa > 1:4325507/1‑71 (MQ=255) gCCGAGGCCAAATAAATCAGCGGAAACGATATTCACCAGGtt < 1:4819783/42‑1 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:5066925/1‑40 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:4091978/1‑40 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:4005825/1‑40 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:3708164/1‑40 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:1260618/1‑40 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:3288686/1‑40 (MQ=255) gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg < 1:15690/37‑1 (MQ=255) gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa < 1:303257/46‑1 (MQ=255) ccgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCaa < 1:4561973/67‑1 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa < 1:5329182/45‑1 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:61551/71‑1 (MQ=255) cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAta < 1:1027594/69‑2 (MQ=255) cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAt > 1:568668/3‑70 (MQ=255) gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa < 1:1074104/70‑3 (MQ=255) gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa < 1:2907473/70‑3 (MQ=255) aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCa < 1:254872/67‑1 (MQ=255) aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGa < 1:2147117/71‑1 (MQ=255) aagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGc < 1:2102362/65‑1 (MQ=255) aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa < 1:1532620/71‑1 (MQ=255) aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg < 1:2897764/46‑1 (MQ=255) aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt > 1:343780/1‑71 (MQ=255) aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt > 1:2210402/1‑71 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa > 1:4757351/1‑40 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa > 1:627901/1‑40 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTgg > 1:5434049/1‑53 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc < 1:1474192/71‑1 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc < 1:1231880/71‑1 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc > 1:8113/1‑71 (MQ=255) aTCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCgg > 1:25208/1‑71 (MQ=255) cAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg > 1:3068564/1‑51 (MQ=255) aggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:920563/54‑1 (MQ=255) | ATCGCGGCGATATCCACCGGCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGG > minE/2596038‑2596170 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |