| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,596,127 | G→A | 29.7% | S157S (TCC→TCT) | arsB ← | arsenite/antimonite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,596,127 | 0 | G | A | 29.7% | 119.5 / 60.4 | 88 | S157S (TCC→TCT) | arsB | arsenite/antimonite transporter |
| Reads supporting (aligned to +/- strand): ref base G (31/30); new base A (12/14); total (44/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTA > minE/2596057‑2596193 | gCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg < 1:3655638/71‑1 (MQ=255) cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa > 1:3207204/1‑69 (MQ=255) cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaaa > 1:2358490/1‑69 (MQ=255) cATAACGGAGGCGTACT‑GCGCAAAGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAgaa > 1:4646796/1‑69 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg > 1:467677/1‑63 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa > 1:2641538/1‑70 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt < 1:249723/71‑1 (MQ=255) aCCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt < 1:1330378/71‑1 (MQ=255) cGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt > 1:3443319/1‑69 (MQ=255) gCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg > 1:1004157/1‑71 (MQ=255) cGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGc < 1:4355585/71‑1 (MQ=255) aTTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa < 1:1196447/62‑1 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg > 1:2173524/1‑52 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg > 1:4434754/1‑52 (MQ=255) ttCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGAAATTCACAAGGTTGGAGACAATAAGCGGCAgg > 1:2493236/1‑70 (MQ=255) tCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg > 1:5291843/1‑62 (MQ=255) cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg > 1:3387305/4‑71 (MQ=255) cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg > 1:3393996/4‑71 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc > 1:1196194/1‑71 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc < 1:3513256/71‑1 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc > 1:351590/1‑71 (MQ=255) cg‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc < 1:1877612/71‑1 (MQ=255) g‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg < 1:3270281/71‑1 (MQ=255) caaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACa < 1:5531407/52‑1 (MQ=255) aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg < 1:11626/60‑1 (MQ=255) aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg < 1:3444343/60‑1 (MQ=255) aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTTGAGACAATAAGCGGCAGGCTGGCgg > 1:2759706/1‑71 (MQ=255) aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTa > 1:4325507/1‑71 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:3288686/1‑40 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:4091978/1‑40 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:3708164/1‑40 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:4005825/1‑40 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:1260618/1‑40 (MQ=255) ggCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGga > 1:5066925/1‑40 (MQ=255) gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTg < 1:15690/37‑1 (MQ=255) gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa < 1:303257/46‑1 (MQ=255) ccgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCaa < 1:4561973/67‑1 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa < 1:5329182/45‑1 (MQ=255) ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:61551/71‑1 (MQ=255) cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAta < 1:1027594/69‑2 (MQ=255) cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAt > 1:568668/3‑70 (MQ=255) gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa < 1:2907473/70‑3 (MQ=255) gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa < 1:1074104/70‑3 (MQ=255) aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCa < 1:254872/67‑1 (MQ=255) aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGa < 1:2147117/71‑1 (MQ=255) aagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGc < 1:2102362/65‑1 (MQ=255) aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa < 1:1532620/71‑1 (MQ=255) aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg < 1:2897764/46‑1 (MQ=255) aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt > 1:343780/1‑71 (MQ=255) aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt > 1:2210402/1‑71 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa > 1:627901/1‑40 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa > 1:4757351/1‑40 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTgg > 1:5434049/1‑53 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc < 1:1231880/71‑1 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc > 1:8113/1‑71 (MQ=255) gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc < 1:1474192/71‑1 (MQ=255) aTCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCgg > 1:25208/1‑71 (MQ=255) cAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg > 1:3068564/1‑51 (MQ=255) aggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc < 1:920563/54‑1 (MQ=255) gagaCGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAt > 1:618636/4‑71 (MQ=255) gagaCGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAt > 1:5038161/4‑71 (MQ=255) gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt < 1:332899/71‑1 (MQ=255) gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt < 1:5361754/71‑1 (MQ=255) aCGATATTCACCAGGTTGGAGACAATAAGCGTCAGCCt > 1:1199707/1‑38 (MQ=38) cGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACg < 1:1992297/71‑1 (MQ=255) cGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACg < 1:5620776/71‑1 (MQ=255) cGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAca < 1:2088555/71‑2 (MQ=255) gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc > 1:580845/1‑39 (MQ=255) gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgccgcc < 1:1043670/64‑1 (MQ=255) gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGa < 1:1039141/71‑1 (MQ=255) gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGa < 1:1032259/71‑1 (MQ=255) atatTCACCAGGTTGGAGACAATAAGCGGCAGGCTgg > 1:3356018/1‑37 (MQ=255) atTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATc > 1:2517566/1‑53 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTAt > 1:1198090/1‑40 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:86279/47‑1 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt < 1:5580356/47‑1 (MQ=255) ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc < 1:2205322/71‑1 (MQ=255) ttCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc < 1:2890870/71‑1 (MQ=255) ttCACCAGCTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAt < 1:2379629/62‑1 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGcc < 1:5543577/70‑1 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGcc < 1:4629170/70‑1 (MQ=255) cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc > 1:3401340/1‑69 (MQ=255) cACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGcc < 1:2777360/70‑1 (MQ=255) cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg < 1:1548326/71‑1 (MQ=255) cAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtg > 1:3791224/1‑69 (MQ=255) aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt > 1:3866034/1‑56 (MQ=255) aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt > 1:3513260/1‑56 (MQ=255) aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCa > 1:2594433/1‑67 (MQ=255) ggTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg > 1:4563082/1‑69 (MQ=255) ggTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgtc > 1:1878442/1‑70 (MQ=255) ggTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgtc > 1:2786185/1‑70 (MQ=255) gTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa < 1:4699307/59‑3 (MQ=255) gTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtg < 1:204203/68‑3 (MQ=255) ggAGACAATAAGCGGCAGGCTGGCGGTATCGGaaa > 1:995273/1‑35 (MQ=255) | GCACCATCACCGAGGCGTATTCGCG‑AAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTA > minE/2596057‑2596193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |