Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,596,130 G→A 28.7% V156V (GTC→GTT arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,596,1300GA28.7% 114.7 / 53.0 80V156V (GTC→GTTarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (27/30);  new base A (9/14);  total (36/44)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTT  >  minE/2596065‑2596200
                                                                 |                                                                      
aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACaa                                                                    >  1:2641538/1‑70 (MQ=255)
aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                                                                   <  1:249723/71‑1 (MQ=255)
aCCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                                                                   <  1:1330378/71‑1 (MQ=255)
  cGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAAt                                                                   >  1:3443319/1‑69 (MQ=255)
      gCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg                                                             >  1:1004157/1‑71 (MQ=255)
       cGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGc                                                            <  1:4355585/71‑1 (MQ=255)
          aTTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                                                  <  1:1196447/62‑1 (MQ=255)
           ttCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGAAATTCACAAGGTTGGAGACAATAAGCGGCAgg                                                         >  1:2493236/1‑70 (MQ=255)
            tCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAg                                                                >  1:5291843/1‑62 (MQ=255)
              cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg                                                     >  1:3387305/4‑71 (MQ=255)
              cgcaaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTgg                                                     >  1:3393996/4‑71 (MQ=255)
               cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                                                    >  1:1196194/1‑71 (MQ=255)
               cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                                                    >  1:351590/1‑71 (MQ=255)
               cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                                                    <  1:3513256/71‑1 (MQ=255)
               cgAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                                                    <  1:1877612/71‑1 (MQ=255)
                gAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCg                                                   <  1:3270281/71‑1 (MQ=255)
                caaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACa                                                                     <  1:5531407/52‑1 (MQ=255)
                 aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg                                                             <  1:11626/60‑1 (MQ=255)
                 aaaGCTCAGACCGAAGAAGTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCgg                                                             <  1:3444343/60‑1 (MQ=255)
                 aaaGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTTGAGACAATAAGCGGCAGGCTGGCgg                                                  >  1:2759706/1‑71 (MQ=255)
                   aGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTa                                                >  1:4325507/1‑71 (MQ=255)
                          gCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                                                  <  1:303257/46‑1 (MQ=255)
                           ccgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCaa                                         <  1:4561973/67‑1 (MQ=255)
                           ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                                                  <  1:5329182/45‑1 (MQ=255)
                           ccAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt                                        <  1:61551/71‑1 (MQ=255)
                            cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAta                                       <  1:1027594/69‑2 (MQ=255)
                            cgaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAt                                        >  1:568668/3‑70 (MQ=255)
                             gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa                                      <  1:1074104/70‑3 (MQ=255)
                             gaagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAAtaa                                      <  1:2907473/70‑3 (MQ=255)
                             aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCa                                          <  1:254872/67‑1 (MQ=255)
                             aaaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGa                                      <  1:2147117/71‑1 (MQ=255)
                              aagaagTCCGCCGAGACGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGc                                           <  1:2102362/65‑1 (MQ=255)
                              aaGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGaa                                     <  1:1532620/71‑1 (MQ=255)
                               aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCgg                                                             <  1:2897764/46‑1 (MQ=255)
                               aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt                                    >  1:2210402/1‑71 (MQ=255)
                               aGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAAt                                    >  1:343780/1‑71 (MQ=255)
                                gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                                                  >  1:4757351/1‑40 (MQ=255)
                                gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATa                                                                  >  1:627901/1‑40 (MQ=255)
                                gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTgg                                                     >  1:5434049/1‑53 (MQ=255)
                                gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc                                   <  1:1231880/71‑1 (MQ=255)
                                gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc                                   <  1:1474192/71‑1 (MQ=255)
                                gAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATc                                   >  1:8113/1‑71 (MQ=255)
                                   aTCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCgg                                >  1:25208/1‑71 (MQ=255)
                                     cAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCgg                                                  >  1:3068564/1‑51 (MQ=255)
                                        aggAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGc                                           <  1:920563/54‑1 (MQ=255)
                                          gagaCGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAt                         >  1:5038161/4‑71 (MQ=255)
                                          gagaCGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAt                         >  1:618636/4‑71 (MQ=255)
                                          gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt                         <  1:332899/71‑1 (MQ=255)
                                          gAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt                         <  1:5361754/71‑1 (MQ=255)
                                             aCGATATTCACCAGGTTGGAGACAATAAGCGTCAGCCt                                                       >  1:1199707/1‑38 (MQ=38)
                                              cGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACg                     <  1:1992297/71‑1 (MQ=255)
                                              cGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACg                     <  1:5620776/71‑1 (MQ=255)
                                              cGATATTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAca                     <  1:2088555/71‑2 (MQ=255)
                                               gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGc                                                    >  1:580845/1‑39 (MQ=255)
                                               gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGgccgcc                           <  1:1043670/64‑1 (MQ=255)
                                               gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGa                    <  1:1039141/71‑1 (MQ=255)
                                               gATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGa                    <  1:1032259/71‑1 (MQ=255)
                                                atatTCACCAGGTTGGAGACAATAAGCGGCAGGCTgg                                                     >  1:3356018/1‑37 (MQ=255)
                                                  atTCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATc                                   >  1:2517566/1‑53 (MQ=255)
                                                   ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTAt                                               >  1:1198090/1‑40 (MQ=255)
                                                   ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt                                        <  1:86279/47‑1 (MQ=255)
                                                   ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAAt                                        <  1:5580356/47‑1 (MQ=255)
                                                   ttCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc                <  1:2205322/71‑1 (MQ=255)
                                                   ttCACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGc                <  1:2890870/71‑1 (MQ=255)
                                                   ttCACCAGCTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCAt                         <  1:2379629/62‑1 (MQ=255)
                                                     cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGcc               <  1:4629170/70‑1 (MQ=255)
                                                     cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGcc               <  1:5543577/70‑1 (MQ=255)
                                                     cACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGc                >  1:3401340/1‑69 (MQ=255)
                                                     cACCAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGcc               <  1:2777360/70‑1 (MQ=255)
                                                        cAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg           <  1:1548326/71‑1 (MQ=255)
                                                        cAGGTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtg           >  1:3791224/1‑69 (MQ=255)
                                                         aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt                         >  1:3866034/1‑56 (MQ=255)
                                                         aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCAt                         >  1:3513260/1‑56 (MQ=255)
                                                         aGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCa              >  1:2594433/1‑67 (MQ=255)
                                                          ggTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCg           >  1:4563082/1‑69 (MQ=255)
                                                          ggTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgtc         >  1:1878442/1‑70 (MQ=255)
                                                          ggTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtgtc         >  1:2786185/1‑70 (MQ=255)
                                                           gTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGAcaa                    <  1:4699307/59‑3 (MQ=255)
                                                           gTTAGAAACAATGAGCGGCAGGCTGGCCGTATCTGCAATAAATCCTGCAGCCATGACAAAGGCCAgtg           <  1:204203/68‑3 (MQ=255)
                                                              ggAGACAATAAGCGGCAGGCTGGCGGTATCGGaaa                                         >  1:995273/1‑35 (MQ=255)
                                                               gagaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCg                                 <  1:4551296/42‑1 (MQ=255)
                                                               gagaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGcc    <  1:2455605/71‑1 (MQ=255)
                                                                 gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt  <  1:5668941/71‑1 (MQ=255)
                                                                 gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt  <  1:3078189/71‑1 (MQ=255)
                                                                 gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt  <  1:2713506/71‑1 (MQ=255)
                                                                 gaCAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCtt  <  1:1011863/71‑1 (MQ=255)
                                                                 |                                                                      
ACCGAGGCGTATTCGCGAAAGCCGAGGCCAAAGAAATCAGCGGAAACGATATTCACCAGGTTGGAGACAATAAGCGGCAGGCTGGCGGTATCGGCAATGAATCCGGCCGCCATCACGAACGCCAGCGTAGTGCCTT  >  minE/2596065‑2596200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: