| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,608,096 | T→C | 35.6% | intergenic (‑206/+104) | pitA ← / ← nikR | phosphate transporter, low‑affinity/DNA‑binding transcriptional regulator, Ni‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,608,096 | 0 | T | C | 35.6% | 35.1 / 38.8 | 45 | intergenic (‑206/+104) | pitA/nikR | phosphate transporter, low‑affinity/DNA‑binding transcriptional regulator, Ni‑binding |
| Reads supporting (aligned to +/- strand): ref base T (15/14); new base C (11/5); total (26/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01 | |||||||||||
TCAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCCCTT > minE/2608028‑2608165 | tCAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGccc > 1:3930310/1‑71 (MQ=255) cAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCg > 1:1374382/1‑71 (MQ=255) cAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCg > 1:857431/1‑71 (MQ=255) cAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCg > 1:1915611/1‑71 (MQ=255) ggCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGtt > 1:3893897/1‑71 (MQ=255) aaaTACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGttttt > 1:644422/1‑71 (MQ=255) aTACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtttttt > 1:4923204/1‑70 (MQ=255) aTACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtttttt > 1:2118391/1‑70 (MQ=255) tACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtttttt‑t > 1:4453845/1‑70 (MQ=255) tACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtttttt > 1:403954/1‑69 (MQ=255) tACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑Ta < 1:4770038/71‑1 (MQ=255) ccTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAaga < 1:3109219/69‑1 (MQ=255) ccTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAagag > 1:305275/1‑71 (MQ=255) ccTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAaga < 1:673437/70‑1 (MQ=255) atatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTc > 1:3669960/1‑71 (MQ=255) atatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAaga > 1:4937412/1‑60 (MQ=255) atatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAAcg > 1:4228536/1‑70 (MQ=255) atatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAAcg > 1:631526/1‑70 (MQ=255) tatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCg < 1:2676509/71‑1 (MQ=255) ttGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGa > 1:5236651/1‑71 (MQ=255) ttGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGa > 1:1057640/1‑71 (MQ=255) ttGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAAcgtg < 1:2997344/70‑4 (MQ=255) gATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGa > 1:2577980/1‑69 (MQ=255) gTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAaga < 1:4810235/47‑1 (MQ=255) gTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAaga < 1:874692/47‑1 (MQ=255) gTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAaga < 1:3617268/47‑1 (MQ=255) gTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTAtt < 1:2300249/71‑1 (MQ=255) gTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAACGTGGAAAggttt > 1:2578678/1‑67 (MQ=255) gTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAACGTGGAAAggttt < 1:4867585/70‑4 (MQ=255) gACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTaga < 1:5421076/71‑1 (MQ=255) gACCACTGAGGAATCTTTACAATTCAAGCCCGTTTTTTCTAAgagga < 1:4595341/47‑1 (MQ=255) ccACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTagaga < 1:485510/71‑1 (MQ=255) tGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATa < 1:5499600/54‑1 (MQ=255) tGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATa < 1:64158/54‑1 (MQ=255) tGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATa < 1:1322613/54‑1 (MQ=255) gAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAACGTGGAAAggtt > 1:1562825/1‑56 (MQ=255) aTCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGa > 1:3811757/1‑46 (MQ=255) aTCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGa > 1:5631197/1‑46 (MQ=255) aTCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGa > 1:827686/1‑46 (MQ=255) cTTTACAATTCACGCCCGTTTTTTCTAAGAGGAgcgc < 1:3927858/37‑1 (MQ=255) tttACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCaa < 1:3927597/37‑1 (MQ=255) tttACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCaa < 1:1736691/37‑1 (MQ=255) ttACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGc > 1:3951402/1‑71 (MQ=255) cACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGcc > 1:4621154/1‑71 (MQ=255) cACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGcc > 1:815398/1‑71 (MQ=255) gTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCCCtt > 1:3232550/1‑71 (MQ=255) | TCAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCCCTT > minE/2608028‑2608165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |