| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,561 | C→T | 16.8% | Q104Q (CAG→CAA) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,561 | 0 | C | T | 16.8% | 250.0 / 33.8 | 101 | Q104Q (CAG→CAA) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (50/34); new base T (11/6); total (61/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCA > minE/312491‑312627 | tCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAcc > 1:2304822/1‑71 (MQ=255) aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCt < 1:1199429/70‑1 (MQ=255) aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg > 1:124613/1‑71 (MQ=255) aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg > 1:444578/1‑71 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcg > 1:5373523/1‑71 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcg > 1:3268437/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:1201616/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc < 1:1253011/71‑1 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:3131442/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:1830243/1‑71 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcg > 1:630402/1‑70 (MQ=255) tgctgcTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGa > 1:2052532/1‑70 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGata > 1:3822746/1‑71 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGata > 1:5065724/1‑71 (MQ=255) ctgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGatat > 1:3947693/1‑71 (MQ=255) ctgAACTTCTTGCGGCAGCACCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGCACCTGCGCGATATCCg > 1:2945349/1‑71 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:448469/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:4642006/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:515958/1‑70 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCt > 1:2598920/1‑54 (MQ=255) ttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:5200020/1‑71 (MQ=255) tGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:4264842/1‑70 (MQ=255) tGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:2379489/1‑70 (MQ=255) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtc < 1:2828131/68‑2 (MQ=16) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:233171/1‑70 (MQ=16) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:3609367/1‑70 (MQ=16) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTa < 1:4587399/68‑1 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc < 1:5343728/69‑1 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:4298404/1‑69 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:2560355/1‑69 (MQ=255) cGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccgg < 1:4162183/71‑3 (MQ=16) cGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg < 1:4544287/70‑2 (MQ=16) cGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCa < 1:3411071/70‑1 (MQ=255) agcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtg < 1:4679745/71‑2 (MQ=16) agcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGa < 1:409901/68‑1 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:4628002/71‑1 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:3845462/71‑1 (MQ=255) caACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtgga < 1:3355794/71‑4 (MQ=12) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATc > 1:2660822/1‑51 (MQ=255) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGa < 1:3765029/66‑1 (MQ=255) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:1095740/71‑1 (MQ=255) aaCGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtgg > 1:4200181/1‑67 (MQ=12) aaCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:3928987/70‑1 (MQ=255) aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:1332078/1‑71 (MQ=255) cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg > 1:5432914/1‑71 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:3842056/1‑69 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:1776993/71‑1 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:1006632/1‑71 (MQ=255) gCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTc < 1:480879/71‑1 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg > 1:4784177/1‑71 (MQ=255) tCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg > 1:4466570/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:2117113/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:5243350/1‑71 (MQ=255) ccAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:1625862/71‑1 (MQ=255) aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa < 1:3901193/70‑1 (MQ=255) aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt < 1:2939575/71‑1 (MQ=255) gCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtc > 1:5688232/1‑46 (MQ=16) ggaGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGAc > 1:1986088/3‑50 (MQ=12) gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCa > 1:3125337/1‑41 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGc < 1:4687738/70‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa < 1:3328822/71‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa < 1:5134130/71‑1 (MQ=255) aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg > 1:444222/1‑59 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg < 1:165212/52‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg < 1:208285/59‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg < 1:159424/59‑1 (MQ=255) tGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:5196927/1‑40 (MQ=12) tGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTccc > 1:2778589/1‑40 (MQ=12) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:809661/47‑1 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:4119164/1‑71 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:692386/1‑71 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:1367844/1‑71 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:2858834/1‑71 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:2388103/1‑71 (MQ=255) ttCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGTAATTGTCACGCTAcc > 1:183813/1‑71 (MQ=255) tCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGTAATTGTCACGCTAccg > 1:3060230/1‑70 (MQ=255) tCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACgg < 1:1061429/66‑1 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc < 1:3835512/70‑1 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc < 1:5346171/70‑1 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc > 1:2931164/1‑70 (MQ=255) tGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa < 1:2028869/49‑1 (MQ=255) tGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa < 1:2099292/49‑1 (MQ=255) gCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg > 1:2156103/1‑71 (MQ=255) cACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg > 1:1186796/1‑49 (MQ=255) cACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg > 1:1963865/1‑49 (MQ=255) cACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg < 1:4528965/70‑1 (MQ=255) ccTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc < 1:4420204/53‑1 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:342486/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:5405150/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:1548633/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:2754127/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:4096186/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt > 1:4291963/1‑70 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa > 1:5314578/1‑71 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa > 1:5319846/1‑71 (MQ=255) aCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:5263897/45‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:3187097/62‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:2092730/62‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:3717452/62‑1 (MQ=255) cTGCGCGATATCAGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:2477612/62‑1 (MQ=255) | TCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCA > minE/312491‑312627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |