| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,662,853 | T→G | 6.3% | intergenic (‑25/‑164) | rtcR ← / → rtcB | sigma 54‑dependent transcriptional regulator of rtcBA expression/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,662,853 | 0 | T | G | 6.3% | 204.4 / 5.0 | 78 | intergenic (‑25/‑164) | rtcR/rtcB | sigma 54‑dependent transcriptional regulator of rtcBA expression/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (33/40); new base G (2/3); total (35/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCCAGTACGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACGGACGTAAAGATATAATCTTTTTT > minE/2662784‑2662921 | aTCCAGTACGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGata > 1:59063/1‑71 (MQ=255)aTCCAGTACGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGata > 1:2069659/1‑71 (MQ=255)aTCCAGTACGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGata > 1:2703249/1‑71 (MQ=255) cAGTACGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATAttt > 1:1903551/1‑71 (MQ=255) cAGTACGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATAttt < 1:2779685/71‑1 (MQ=255) aCGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCAt > 1:942755/1‑70 (MQ=255) cGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCAtt < 1:1676593/70‑1 (MQ=255) cGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCAtt < 1:2782211/70‑1 (MQ=255) cGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTa < 1:2124237/71‑1 (MQ=255) cGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTa > 1:3656181/1‑71 (MQ=255) cGACAAAGCCAAAAGCGACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTa < 1:1705867/71‑1 (MQ=255) gACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTc > 1:2226927/1‑60 (MQ=255) aCAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGt < 1:1146851/68‑1 (MQ=255) aaagccaaaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATAttt < 1:3429813/56‑1 (MQ=255) aaagccaaaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATAttt < 1:5025205/56‑1 (MQ=255) aaagccaaaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTc > 1:1368732/1‑57 (MQ=255) aagccaaaagccaCTGTTTTACTCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTg < 1:3111058/71‑1 (MQ=255) aagccaaaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTg < 1:3192046/71‑1 (MQ=255) aagccaaaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTg > 1:4572051/1‑71 (MQ=255) agccaaaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTgg > 1:1486444/1‑71 (MQ=255) caaaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCtt > 1:3703833/1‑71 (MQ=255) caaaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCtt > 1:1860532/1‑71 (MQ=255) aaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCAtt < 1:4661556/53‑1 (MQ=255) aaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTAt > 1:3494873/1‑55 (MQ=255) aaagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTg < 1:5627366/71‑1 (MQ=255) aagccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGc < 1:4933504/71‑1 (MQ=255) agccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCg < 1:2357371/71‑1 (MQ=255) agccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCg < 1:4324270/71‑1 (MQ=255) agccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCg < 1:3614063/71‑1 (MQ=255) gccaCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGAGATTTCATTATCGGTAAAtcg < 1:4545447/62‑3 (MQ=255) caCTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCggg < 1:4702398/70‑1 (MQ=255) tGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAAt > 1:1734513/1‑71 (MQ=255) tGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAAt > 1:1478383/1‑71 (MQ=255) gTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCAt > 1:1111529/1‑43 (MQ=255) gTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCAt > 1:1230870/1‑43 (MQ=255) gTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGc < 1:3583512/58‑1 (MQ=255) gTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGc < 1:4968941/58‑1 (MQ=255) ttttACGCATCTTAGATATCCTTATAAAAGACGATAttt < 1:3705698/39‑1 (MQ=255) ttttACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAAtc < 1:3434125/70‑1 (MQ=255) tttACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAAtct > 1:3723354/1‑70 (MQ=255) ttACGCATCTTAGATATCCTTATAAAAGACGATATTTCa > 1:351370/1‑39 (MQ=255) ttACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAAt > 1:3513199/1‑51 (MQ=255) ttACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGaaa > 1:5109758/1‑66 (MQ=255) ttACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAAtctc < 1:4391898/70‑1 (MQ=255) tACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTcc < 1:5048090/70‑1 (MQ=255) tACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTcc < 1:2869614/70‑1 (MQ=255) tACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTcc < 1:1698806/70‑1 (MQ=255) tACGCATCTTAGATATCCTTATAAAAGACGAGATTTCATTATCGTTAAATCGGCTTTGCGGGAAATCTcc < 1:689451/70‑1 (MQ=255) tACGCATCTTAGATATCCTTATAAAAGACGAGATTTCATTATCGGTAAATCGGCTTTGCGGGAAATCTcc < 1:4562811/70‑1 (MQ=255) aCGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCg > 1:1312398/1‑70 (MQ=255) gCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGtt < 1:4430214/71‑1 (MQ=255) tCTTAGATATCCTTATAAAAGACGATATTTCATTAt < 1:3024991/36‑1 (MQ=255) tCTTAGATATCCTTATAAAAGACGATATTTCATTAt < 1:4468802/36‑1 (MQ=255) tCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTa < 1:590022/41‑1 (MQ=255) tCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTgg < 1:1641511/47‑1 (MQ=255) tCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCt > 1:4344058/1‑49 (MQ=255) tCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCt > 1:2250792/1‑49 (MQ=255) cTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTa < 1:229228/40‑1 (MQ=255) cTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTa < 1:5693997/40‑1 (MQ=255) ttAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAAt > 1:2868786/1‑42 (MQ=255) ttAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAAt > 1:5324664/1‑42 (MQ=255) ttAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCg > 1:1521712/1‑52 (MQ=255) ttAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTAc > 1:4710040/1‑68 (MQ=255) tAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACGGAc > 1:142300/1‑71 (MQ=255) aGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACgg < 1:634806/68‑1 (MQ=255) gATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACGGACg < 1:1063710/70‑1 (MQ=255) atatCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACGGACGTa < 1:1375955/71‑1 (MQ=255) ccTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGaaa > 1:729746/1‑49 (MQ=255) cTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACGGACGTAAAGa > 1:1647707/1‑70 (MQ=255) taAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCggg < 1:4779068/41‑1 (MQ=255) aaGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACg > 1:5165286/1‑53 (MQ=255) aaGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACg > 1:2608953/1‑53 (MQ=255) aaGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACg > 1:3197803/1‑53 (MQ=255) aaGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACGGACGTAAAGATATAATCt > 1:4464027/1‑71 (MQ=255) aGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACGGACGTAAAGATATAATCtt < 1:137904/71‑1 (MQ=255) aCGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAAtctc < 1:2744316/42‑1 (MQ=255) aCGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAAtctc < 1:3631012/42‑1 (MQ=255) aCGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACGGACGTaa > 1:1277313/1‑57 (MQ=255) cGATATTTCATTATCTGTAAATTGGCTTTGCGGGa < 1:3941655/35‑1 (MQ=255) cGAGATTTCATTATCGGTAAATCGGCTTTGCGGGAAATCTCCGTTTACGGACGTAAAAAtata > 1:5322624/1‑63 (MQ=255) cGAGATTTCATTATCGGTAAATCGGCTTTGCGGGAAATCTCCGTTTACGGACGTAAAAAtata > 1:2565239/1‑63 (MQ=255) atatTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACGGACGTAAAGATATAATCtttttt < 1:600098/70‑1 (MQ=255) | ATCCAGTACGGTACCGACAAAGCCAAAAGCCACTGTTTTACGCATCTTAGATATCCTTATAAAAGACGATATTTCATTATCGGTAAATTGGCTTTGCGGGAAATCTCCGGTTACGGACGTAAAGATATAATCTTTTTT > minE/2662784‑2662921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |