Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,182	A→G	6.0%	L250L (TTG→CTG)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,182	0	A	G	6.0%	236.6 / 4.3	83	L250L (TTG→CTG)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand):  ref base A (47/31);  new base G (2/3);  total (49/34)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

AGAGGAACTCCGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGTCGATAAAATCG  >  minE/2677112‑2677252                                                                       |                                                                       agagGAACTCCGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCaa                                                                        <  1:2575114/71‑1 (MQ=255)   agGAACTCCGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATc                                                                      >  1:4063120/1‑71 (MQ=255)   agGAACTCCGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATc                                                                      >  1:3406544/1‑71 (MQ=255)          ccGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCg                                                                >  1:2233742/1‑70 (MQ=255)          ccGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCg                                                                >  1:4432514/1‑70 (MQ=255)          ccGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCg                                                                >  1:2227312/1‑70 (MQ=255)           cGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATc                                                                      >  1:469898/1‑63 (MQ=255)           cGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATc                                                                      >  1:3592952/1‑63 (MQ=255)           cGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGc                                                               >  1:3978426/1‑70 (MQ=255)            ggATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCaa                                                             <  1:2413551/71‑1 (MQ=255)                tCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTc                                                                  >  1:477769/1‑62 (MQ=255)                tCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGt                                                         >  1:4851246/1‑71 (MQ=255)                    gCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCAt                                                      >  1:1679226/1‑70 (MQ=255)                     cATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTa                                                    <  1:3722993/71‑1 (MQ=255)                      attattTTGGCTATATGCAGGGC‑TTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTaaa                                                  >  1:1993270/1‑71 (MQ=255)                       ttattTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTaaa                                                  <  1:3674607/71‑1 (MQ=255)                       ttattTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTaaa                                                  <  1:2999804/71‑1 (MQ=255)                         attTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATc                                                                      >  1:497589/1‑49 (MQ=255)                         attTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAAct                                                >  1:1442851/1‑71 (MQ=255)                         attTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAAc                                                 >  1:1614431/1‑70 (MQ=255)                            ttGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAActct                                              <  1:4030237/70‑1 (MQ=255)                               gCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCtt                                          <  1:2238824/71‑1 (MQ=255)                                  atatGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCaa                                                             >  1:2286552/1‑49 (MQ=255)                                  atatGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTtgtg                                       >  1:3018182/1‑71 (MQ=255)                                   tatGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCtt                                                                    <  1:865956/41‑1 (MQ=255)                                   tatGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTgtgt                                      >  1:2640550/1‑71 (MQ=255)                                     tGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAAt                                                                       <  1:985862/36‑1 (MQ=255)                                     tGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTa                                                    >  1:663721/1‑55 (MQ=255)                                     tGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAActc                                               <  1:2322354/60‑1 (MQ=255)                                     tGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGttt                                    >  1:5674497/1‑71 (MQ=255)                                     tGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGttt                                    >  1:458701/1‑71 (MQ=255)                                     tGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGttt                                    >  1:2039009/1‑71 (MQ=255)                                      gCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCttt                                                                   <  1:4568646/39‑1 (MQ=255)                                      gCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTc                                   >  1:2855571/1‑71 (MQ=255)                                       cAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCg                                  >  1:2114348/1‑71 (MQ=255)                                        aGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTcc                                                                 >  1:5476994/1‑39 (MQ=255)                                        aGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCgg                                 <  1:3123443/71‑1 (MQ=255)                                         gggCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTc                                                        <  1:1161130/47‑1 (MQ=255)                                         gggCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGttt                                    <  1:4605188/67‑1 (MQ=255)                                          ggCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGt                                >  1:2284974/1‑70 (MQ=255)                                            cTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGTAATAGTCATTAAACTCTCCTTGTGTTTCGGTGaa                             >  1:665471/1‑71 (MQ=255)                                            cTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTaaa                                                  >  1:3730373/1‑50 (MQ=255)                                            cTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTg                               <  1:2654664/69‑1 (MQ=255)                                            cTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGaa                             >  1:5566757/1‑71 (MQ=255)                                             tttGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTa                                                    <  1:1186435/47‑1 (MQ=255)                                              ttGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGaa                             >  1:1695325/1‑69 (MQ=255)                                               tGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGt                          >  1:2339278/1‑71 (MQ=255)                                               tGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGt                          >  1:3608937/1‑71 (MQ=255)                                               tGGATTGTTCCACCTCCTGCCGCAATATTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGt                          >  1:3721111/1‑71 (MQ=255)                                                 gATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAg                                                          >  1:1901807/1‑37 (MQ=255)                                                 gATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGtt                         <  1:2158191/70‑1 (MQ=255)                                                 gATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTc                        >  1:1466136/1‑71 (MQ=255)                                                   ttGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCa                                                       <  1:1809776/38‑1 (MQ=255)                                                   ttGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCgg                      <  1:3575475/71‑1 (MQ=255)                                                   ttGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCgg                      <  1:2933328/71‑1 (MQ=255)                                                    tGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTaaa                                                  >  1:1461287/1‑42 (MQ=255)                                                     gTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCAtt                                                     >  1:4177365/1‑38 (MQ=255)                                                     gTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTa                                                    <  1:4398553/39‑1 (MQ=255)                                                     gTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGc                     <  1:1473989/70‑1 (MQ=255)                                                     gTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGc                     <  1:2483895/70‑1 (MQ=255)                                                      ttCCCACTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCa                    <  1:1604583/70‑1 (MQ=255)                                                       tCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTaaa                                                  >  1:2550433/1‑39 (MQ=255)                                                       tCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCg                       >  1:783954/1‑66 (MQ=255)                                                       tCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCg                       >  1:4082537/1‑66 (MQ=255)                                                       tCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCaa                   >  1:5549790/1‑70 (MQ=255)                                                       tCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAAc                  >  1:3991758/1‑71 (MQ=255)                                                       tCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAAc                  >  1:2568676/1‑71 (MQ=255)                                                         ccccTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACg                 <  1:1969183/70‑1 (MQ=255)                                                          cccTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTACTTGTGTTTCGGTg                               <  1:4651551/55‑1 (MQ=255)                                                            ttcctGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTtttg                                       <  1:4111483/44‑1 (MQ=12)                                                            ttcctGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTttgta                                     >  1:3008479/2‑46 (MQ=12)                                                            ctcctGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCaa                   <  1:3778954/65‑1 (MQ=255)                                                            ctcctGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCaa                   <  1:4730658/65‑1 (MQ=255)                                                            ctcctGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCaa                   <  1:1389454/65‑1 (MQ=255)                                                            ctcctGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCaa                   <  1:2492474/65‑1 (MQ=255)                                                            ctcctGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGt             >  1:1520338/1‑71 (MQ=255)                                                             tcctGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTttgt                                      <  1:5020024/45‑1 (MQ=12)                                                             tcctGCCGTAGCCGTTCAGCAATAGTCATTAATCTCTCCTTttgt                                      <  1:1771849/45‑1 (MQ=12)                                                             tcctGCCGCAGCCGTTCAGCAATAGTCATTAATCTCTCCt                                           >  1:2142348/1‑40 (MQ=16)                                                                 gCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGTCGAt         >  1:3202811/1‑70 (MQ=255)                                                                 gCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGTCGAt         >  1:5014097/1‑70 (MQ=255)                                                                  ccGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTgtgt                                      <  1:1110434/40‑1 (MQ=255)                                                                  ccGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGTCGATaa       <  1:5446177/71‑1 (MQ=255)                                                                    gCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGTCGATaaaa     >  1:1918759/1‑71 (MQ=255)                                                                      aaTCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGTCGATAAAATc   <  1:1136212/71‑1 (MQ=255)                                                                      aaTCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGTCGATAAAATc   <  1:2978835/71‑1 (MQ=255)                                                                       aTCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGTCGATAAAATCg  >  1:1451415/1‑71 (MQ=255)                                                                       aTCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGTCGATAAAATCg  >  1:4838314/1‑71 (MQ=255)                                                                       |                                                                       AGAGGAACTCCGGATTCAAGCATTATTTTGGCTATATGCAGGGCTTTGGATTGTTCCCCCTCCTGCCGCAATCTTTCCGCAATAGTCATTAAACTCTCCTTGTGTTTCGGTGAACGTTCGGCAACGCCGTCGATAAAATCG  >  minE/2677112‑2677252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑