| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,573 | C→A | 14.0% | A100A (GCG→GCT) ‡ | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,573 | 0 | C | A | 14.0% | 356.7 / 25.2 | 120 | A100A (GCG→GCT) ‡ | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (51/52); new base A (10/7); total (61/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 128 total aligned reads displayed.
CTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGT > minE/312504‑312643 | ctgAACTTCTTGCGGCAGCACCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGCACCTGCGCGATATCCg > 1:2945349/1‑71 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:515958/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:4642006/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:448469/1‑70 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg < 1:4296053/69‑1 (MQ=255) tGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:4264842/1‑70 (MQ=255) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtc < 1:870112/68‑2 (MQ=16) gCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:233171/1‑70 (MQ=16) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc < 1:5343728/69‑1 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:2560355/1‑69 (MQ=255) cGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccgg < 1:4162183/71‑3 (MQ=16) cGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg < 1:4544287/70‑2 (MQ=16) cGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCa < 1:3411071/70‑1 (MQ=255) agcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtg < 1:4679745/71‑2 (MQ=16) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:4628002/71‑1 (MQ=255) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGa < 1:3765029/66‑1 (MQ=255) caACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:1095740/71‑1 (MQ=255) aaCGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACtgg > 1:4200181/1‑67 (MQ=12) aaCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:3928987/70‑1 (MQ=255) cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg > 1:5432914/1‑71 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:3842056/1‑69 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:1776993/71‑1 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:1006632/1‑71 (MQ=255) aTCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg > 1:4784177/1‑71 (MQ=255) tCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg > 1:4466570/1‑71 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGt > 1:5243350/1‑71 (MQ=255) aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGa < 1:3901193/70‑1 (MQ=255) aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt < 1:3479724/71‑1 (MQ=255) gCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtc > 1:5688232/1‑46 (MQ=16) ggaGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGAc > 1:1986088/3‑50 (MQ=12) gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCa > 1:3125337/1‑41 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGc < 1:4687738/70‑1 (MQ=255) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa < 1:3328822/71‑1 (MQ=255) aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAg > 1:444222/1‑59 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg < 1:165212/52‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg < 1:208285/59‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAggg < 1:159424/59‑1 (MQ=255) tGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:1841353/1‑40 (MQ=12) tGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccg > 1:5196927/1‑40 (MQ=12) tGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTccc > 1:2778589/1‑40 (MQ=12) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:809661/47‑1 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:1367844/1‑71 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:2388103/1‑71 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:4119164/1‑71 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:2858834/1‑71 (MQ=255) ttCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGTAATTGTCACGCTAcc > 1:183813/1‑71 (MQ=255) tCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGTAATTGTCACGCTAccg > 1:3060230/1‑70 (MQ=255) tCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACgg < 1:1061429/66‑1 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc < 1:3835512/70‑1 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc < 1:5346171/70‑1 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc > 1:2931164/1‑70 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg > 1:515794/1‑71 (MQ=255) tGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa < 1:2099292/49‑1 (MQ=255) gCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg > 1:2156103/1‑71 (MQ=255) cACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg > 1:1186796/1‑49 (MQ=255) cACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAgg > 1:1963865/1‑49 (MQ=255) cACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg < 1:4528965/70‑1 (MQ=255) ccTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc < 1:4420204/53‑1 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:4096186/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:1548633/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:342486/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:5405150/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATc > 1:2754127/1‑51 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt > 1:4291963/1‑70 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa > 1:5319846/1‑71 (MQ=255) aCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:5263897/45‑1 (MQ=255) aCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:2689198/45‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:2092730/62‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:3187097/62‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:3717452/62‑1 (MQ=255) cTGCGCGATATCAGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg < 1:2477612/62‑1 (MQ=255) tGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa < 1:2324496/37‑1 (MQ=255) tGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:3784515/1‑70 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTc > 1:5035813/1‑35 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa > 1:5584548/1‑48 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:1688633/1‑69 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:5504038/1‑69 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:887521/1‑69 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:2221081/1‑69 (MQ=255) cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:3921548/1‑68 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa < 1:4701024/47‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTCCCCGTgg > 1:3660772/1‑54 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt < 1:3748658/57‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt < 1:66138/57‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt < 1:4944176/57‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTag > 1:5381166/1‑66 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa < 1:4340702/70‑1 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa > 1:2985424/1‑70 (MQ=255) atCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGtt < 1:68859/63‑1 (MQ=255) tCCTCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:4604137/38‑1 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:2836004/38‑1 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg < 1:5204235/38‑1 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGc < 1:3000640/39‑1 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAcc > 1:2715166/1‑47 (MQ=255) tCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACAt < 1:4856884/71‑1 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg < 1:2920438/70‑1 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg < 1:1534703/70‑1 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg < 1:4864189/70‑1 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg < 1:5139095/70‑1 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGt < 1:4151029/71‑1 (MQ=255) | CTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGT > minE/312504‑312643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |