| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,708,514 | G→T | 11.3% | intergenic (+52/‑238) | trpS → / → yhfZ | tryptophanyl‑tRNA synthetase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,708,514 | 0 | G | T | 11.3% | 153.1 / 5.9 | 53 | intergenic (+52/‑238) | trpS/yhfZ | tryptophanyl‑tRNA synthetase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (19/28); new base T (3/3); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01 | |||||||||||
TTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGTAGGCCTGATAAGCGTAGCGCATCAGG > minE/2708446‑2708584 | ttGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc > 1:797493/1‑71 (MQ=255) tgtgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGcc < 1:5037687/70‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt < 1:610389/70‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:1204911/71‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:736673/71‑1 (MQ=255) tgGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:186081/71‑1 (MQ=255) cGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCtt < 1:1095803/67‑1 (MQ=255) aaGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:2277731/66‑1 (MQ=255) ccGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGcc > 1:4915527/1‑70 (MQ=255) gTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa > 1:5523793/1‑70 (MQ=255) tAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc > 1:23368/1‑70 (MQ=255) aTTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTa < 1:5000217/67‑1 (MQ=255) ttCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAg < 1:4359624/71‑1 (MQ=255) aTTGTTGGCAAAGTGTGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAt > 1:4534983/1‑52 (MQ=11) aTTGTTGGCAAAGTGTGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAt > 1:5060043/1‑52 (MQ=11) aTTGTTGGCAAAGTGTGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTAt > 1:3300924/1‑52 (MQ=11) ttGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCt > 1:5399815/1‑48 (MQ=255) ttGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGc < 1:1674458/70‑1 (MQ=255) ttGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGc < 1:56510/70‑1 (MQ=255) gCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGcaca > 1:4685801/1‑71 (MQ=255) tGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa < 1:859954/46‑1 (MQ=255) aacAACTTGCGCTTTGTTGATGCCGGATGCGGCGTGAATTCCTTATCCgg < 1:5467278/48‑1 (MQ=11) acttGCGCTTTGTTGATGCCGGATGCGGCGTGAATTCCTTATCCg > 1:3592912/3‑45 (MQ=11) acttGCGCTTTGTTGATGCCGGATGCGGCGTGAATTCCTTATCCg > 1:3127233/3‑45 (MQ=11) acttGCGCTTTGTTGATGCCGGATGCGGCGTGAATTCCTTATCCg > 1:2113157/3‑45 (MQ=11) aaTTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCa < 1:1939108/71‑1 (MQ=255) cttGCGCTTTGTTGATGCCGGATGCGGCGTGAATTCCTTATCCg < 1:2178324/43‑1 (MQ=11) ttGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAg < 1:421498/71‑1 (MQ=255) tGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTaa > 1:580117/1‑55 (MQ=38) gcgcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAgg > 1:4676054/1‑70 (MQ=255) cgcTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGaa < 1:1651142/71‑1 (MQ=255) cTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAAt < 1:3270070/71‑1 (MQ=255) tttGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATg > 1:1982313/1‑71 (MQ=255) tGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATg < 1:4888478/69‑1 (MQ=255) aTGCCGGATGCGGCGTGAACTCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGa < 1:1860145/60‑1 (MQ=255) tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGa < 1:3954048/59‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGTAGGCCTGa > 1:2272304/1‑70 (MQ=255) cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACat < 1:2739686/46‑1 (MQ=255) cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGTAGGCCTGATa < 1:5031994/71‑1 (MQ=255) tGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGTAGGCCTGATAAGCg > 1:3603607/1‑71 (MQ=255) gcggcgTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGTAGGCCTGATa > 1:2940464/1‑66 (MQ=255) cggcgTGAACGCCTTATCCGGCCTACAAAGTAATg > 1:4387541/1‑35 (MQ=255) gcgTGAACGCGTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGTAGGCCTGATAAGCGTAgc < 1:1384881/71‑1 (MQ=255) gcgTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTg < 1:4038131/42‑1 (MQ=255) cgTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGt > 1:3409375/1‑52 (MQ=255) cgTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGTAGGCCTGATAAGCGTAgcg < 1:4653431/71‑1 (MQ=255) tGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCaa < 1:1146221/42‑1 (MQ=255) tGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCaa < 1:1383325/42‑1 (MQ=255) tGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCaa < 1:66273/42‑1 (MQ=255) tGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCaa < 1:1975071/42‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGTAGGCCTGATAAGCGTAGCGCAt > 1:3370133/1‑70 (MQ=255) cGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATg < 1:355489/45‑1 (MQ=255) cGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATg > 1:299035/1‑45 (MQ=255) gCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATg < 1:16924/44‑1 (MQ=255) gCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGTAGGCCTGATAAGCGTAGCGCATCAgg > 1:813129/1‑71 (MQ=255) gCCTTATACGGCCTACAAAGTAATGCACATATTGCAAGGaaa > 1:1495741/1‑42 (MQ=255) | TTGTGGCGAAGCCGTAATTCACTATTGCTGGCAAATTGCGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTAATGCACATATTGCAAGGAAATGTAGGCCTGATAAGCGTAGCGCATCAGG > minE/2708446‑2708584 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |