| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,708,913 | G→A | 78.1% | E54E (GAG→GAA) | yhfZ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,708,913 | 0 | G | A | 78.1% | 113.9 / 29.9 | 64 | E54E (GAG→GAA) | yhfZ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base A (30/20); total (37/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
AGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATA > minE/2708847‑2708975 | aGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATc > 1:4853117/1‑69 (MQ=255)aGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATc > 1:4782177/1‑69 (MQ=255)aGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATc > 1:1432258/1‑69 (MQ=255) gATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAg > 1:3417358/1‑71 (MQ=255) tGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCgg < 1:847574/67‑1 (MQ=255) ggCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGa < 1:1448394/67‑1 (MQ=255) aatgaatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACg < 1:2943138/71‑1 (MQ=255) tgaatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGa > 1:3459342/1‑61 (MQ=255) aatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGAtt > 1:3209483/1‑71 (MQ=255) aatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGAtt > 1:3036705/1‑71 (MQ=255) atgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTg < 1:4978589/71‑1 (MQ=255) tgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTg < 1:1501590/70‑1 (MQ=255) tgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTg < 1:5660691/70‑1 (MQ=255) cGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAAc < 1:5201614/70‑1 (MQ=255) gTTCATCCGTTGGCCTGACGCAGGCCGCTTTGAAAACGCTGGAATCAAGCgg < 1:295796/52‑1 (MQ=255) cATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGt > 1:1564634/1‑71 (MQ=255) cATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGa > 1:1278612/1‑54 (MQ=255) cATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGt > 1:4033568/1‑71 (MQ=255) cATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGt > 1:1989860/1‑71 (MQ=255) aTCCTTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGt < 1:3333137/70‑1 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:1010979/71‑1 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:5140493/71‑1 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:544019/71‑1 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGgcg > 1:1410223/1‑70 (MQ=255) tGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc > 1:2489090/1‑70 (MQ=255) tGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc > 1:5332886/1‑70 (MQ=255) ggCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCaa > 1:2724693/1‑71 (MQ=255) ggCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGt > 1:1410442/1‑63 (MQ=255) ggCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGt > 1:1404947/1‑63 (MQ=255) gCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAAt < 1:3925667/71‑1 (MQ=255) ccTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATg < 1:5284244/71‑1 (MQ=255) aCGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGaa > 1:3828186/1‑51 (MQ=255) aCGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAg > 1:4499871/1‑71 (MQ=255) cGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt > 1:4935379/1‑71 (MQ=255) cGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt > 1:203992/1‑71 (MQ=255) gCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGtt < 1:1168167/71‑1 (MQ=255) gCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt > 1:1385710/1‑70 (MQ=255) gCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt > 1:3402161/1‑70 (MQ=255) gCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt > 1:2416082/1‑70 (MQ=255) ccGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTg > 1:1183657/1‑71 (MQ=255) ccGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTg > 1:5609510/1‑71 (MQ=255) ccGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTg < 1:3074273/71‑1 (MQ=255) cgcgTTGAAAACGCTGGAATCAAGCGGAGCGATACGGAt > 1:2138272/1‑39 (MQ=255) gcgTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGAtt > 1:2002797/1‑39 (MQ=255) gcgTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAg > 1:3847029/1‑62 (MQ=255) gcgTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTgg < 1:4010145/70‑1 (MQ=255) cgTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTc < 1:2477216/71‑1 (MQ=255) tGAAAACGCTGGAATCAAGCTGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTgg > 1:5043449/1‑66 (MQ=255) tGAAAACGCTGGAATCAAGCGGATCGATACGGATTGAACGCCGTGGgcgc < 1:609743/50‑1 (MQ=255) tGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGaa > 1:637766/1‑38 (MQ=255) tGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:560475/50‑1 (MQ=255) gAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt > 1:3028858/1‑58 (MQ=255) gAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTAt > 1:4562422/1‑61 (MQ=255) gAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTc > 1:381545/1‑67 (MQ=255) gAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAg < 1:4815048/70‑1 (MQ=255) gAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAg < 1:3661627/70‑1 (MQ=255) gAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAg < 1:4808684/70‑1 (MQ=255) aaaaCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAgg < 1:3347439/70‑1 (MQ=255) aCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAata < 1:1983704/71‑1 (MQ=255) aCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAata < 1:1965070/71‑1 (MQ=255) aCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAata < 1:5573913/71‑1 (MQ=255) cGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAga > 1:1499004/1‑65 (MQ=255) cGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAga > 1:2567475/1‑65 (MQ=255) gCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTAt > 1:4489624/1‑55 (MQ=255) | AGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATA > minE/2708847‑2708975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |