| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,790,657 | G→T | 9.5% | intergenic (+52/‑723) | pgi → / → ubiC | glucosephosphate isomerase/chorismate pyruvate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,790,657 | 0 | G | T | 9.5% | 125.2 / 2.1 | 52 | intergenic (+52/‑723) | pgi/ubiC | glucosephosphate isomerase/chorismate pyruvate lyase |
| Reads supporting (aligned to +/- strand): ref base G (26/18); new base T (2/3); total (28/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGC > minE/2790587‑2790727 | taAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCg < 1:5656318/71‑1 (MQ=255) aGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATg < 1:338673/71‑1 (MQ=255) tGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTg < 1:1345077/71‑1 (MQ=255) ttAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcg > 1:3604181/1‑71 (MQ=255) ttAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcg > 1:5129117/1‑71 (MQ=255) aaTCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgcg > 1:4125487/1‑71 (MQ=255) tcatcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCgg > 1:727137/1‑70 (MQ=255) tcatcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt > 1:2088485/1‑71 (MQ=255) atcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcg > 1:4069955/1‑71 (MQ=255) atcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc > 1:5487258/1‑70 (MQ=255) tcgATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc > 1:5344568/1‑50 (MQ=255) gATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCtt < 1:1619543/71‑1 (MQ=255) cgaatGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCtt < 1:784617/68‑1 (MQ=11) aatGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTAt < 1:801585/70‑1 (MQ=11) gTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAg < 1:361995/71‑1 (MQ=11) gTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAACAGATGCCTGATGCGACGCTACCGCGTCTTATCa < 1:5478651/70‑1 (MQ=11) tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc < 1:2712815/42‑1 (MQ=255) tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAgg > 1:2971764/1‑71 (MQ=255) tAGGACGGATAAGGCGTTTACGCCGCATCCGGCAACAGATGCCTGATGCGACGCTACCGCGTCTTATCAgg > 1:2454585/1‑71 (MQ=11) tAGGACGGATAAGGCGTTTACGCCGCATCCGGCAACAGATGCCTGATGCGACGCTACCGCGTCTTATCAgg > 1:1950254/1‑71 (MQ=11) ggacGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCATGcc < 1:4841860/68‑1 (MQ=1) gacGGATAAGGCGTTTACGCCGCATCCGGCAACAGATGCCTGATGCGACGCTACCGCGTCTTATCAGGcc > 1:2180215/3‑70 (MQ=14) ccGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCg < 1:166887/46‑1 (MQ=255) cGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCATGCCTAc < 1:5246677/71‑1 (MQ=11) ggATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCATGCCTACa > 1:4784248/1‑71 (MQ=11) tAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCATGCCTACatag > 1:2545967/1‑68 (MQ=11) tAAGGCGTTTACGCCGCATCCGGCAACAGATGCCTGATGCGACGCTACCGCGTCTTATCAGGCCTACaaaa > 1:2750305/1‑68 (MQ=11) ttCACGCCGCATCCGGCAACCTATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc < 1:1398961/65‑1 (MQ=255) ttCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc > 1:4074165/1‑44 (MQ=255) ttCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc < 1:2228975/57‑1 (MQ=255) cACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGccga > 1:4244148/1‑55 (MQ=255) cACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc < 1:2803497/55‑1 (MQ=255) cACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa > 1:5346588/1‑71 (MQ=255) aCGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg > 1:3721529/1‑69 (MQ=255) cgccgcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata < 1:874482/71‑1 (MQ=255) gccgcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc > 1:1057757/1‑47 (MQ=255) ccgcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg < 1:5328310/71‑1 (MQ=255) gcATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa > 1:5204864/1‑45 (MQ=9) gcATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa > 1:3962820/1‑45 (MQ=9) gcATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa > 1:4982818/1‑45 (MQ=9) cATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGGCGATGCCGat < 1:5494557/65‑1 (MQ=255) aTCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa < 1:3225822/71‑1 (MQ=255) tCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa < 1:1666800/70‑1 (MQ=255) tCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAgg < 1:2415744/44‑1 (MQ=2) cGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa > 1:2906940/1‑46 (MQ=255) cGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg < 1:1681247/71‑1 (MQ=255) cGGCAACCGATGCCTGATGCGACGCGGTCGCGTCGTATCAGGCCt > 1:765295/1‑45 (MQ=255) gCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa > 1:11565/1‑35 (MQ=255) aaCCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt < 1:4414672/71‑1 (MQ=255) aCCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTa < 1:1847385/68‑1 (MQ=255) aCCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc > 1:5370321/1‑71 (MQ=255) cGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg < 1:4107343/64‑1 (MQ=255) cGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg > 1:1782458/1‑71 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa > 1:4779939/1‑52 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa < 1:2569914/60‑1 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 1:2539130/71‑1 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 1:2651410/71‑1 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 1:448219/71‑1 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 1:4389140/71‑1 (MQ=255) | TAAAGCGTGGCGCGGTTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGC > minE/2790587‑2790727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |