| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,790,676 | T→A | 12.7% | intergenic (+71/‑704) | pgi → / → ubiC | glucosephosphate isomerase/chorismate pyruvate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,790,676 | 0 | T | A | 12.7% | 141.1 / 15.0 | 65 | intergenic (+71/‑704) | pgi/ubiC | glucosephosphate isomerase/chorismate pyruvate lyase |
| Reads supporting (aligned to +/- strand): ref base T (17/35); new base A (5/3); total (26/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.57e-01 | |||||||||||
TCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACG > minE/2790606‑2790744 | tcatcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt > 1:2088485/1‑71 (MQ=255) atcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcg > 1:4069955/1‑71 (MQ=255) atcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc > 1:5487258/1‑70 (MQ=255) gATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCtt < 1:1619543/71‑1 (MQ=255) cgaatGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCtt < 1:784617/68‑1 (MQ=11) aatGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTAt < 1:801585/70‑1 (MQ=11) gTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAg < 1:361995/71‑1 (MQ=11) gTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAACAGATGCCTGATGCGACGCTACCGCGTCTTATCa < 1:5478651/70‑1 (MQ=11) tAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAgg > 1:2971764/1‑71 (MQ=255) tAGGACGGATAAGGCGTTTACGCCGCATCCGGCAACAGATGCCTGATGCGACGCTACCGCGTCTTATCAgg > 1:1950254/1‑71 (MQ=11) tAGGACGGATAAGGCGTTTACGCCGCATCCGGCAACAGATGCCTGATGCGACGCTACCGCGTCTTATCAgg > 1:2454585/1‑71 (MQ=11) ggacGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCATGcc < 1:4841860/68‑1 (MQ=1) gacGGATAAGGCGTTTACGCCGCATCCGGCAACAGATGCCTGATGCGACGCTACCGCGTCTTATCAGGcc > 1:2180215/3‑70 (MQ=14) cGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCATGCCTAc < 1:5246677/71‑1 (MQ=11) ggATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCATGCCTACa > 1:4784248/1‑71 (MQ=11) tAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCATGCCTACatag > 1:2545967/1‑68 (MQ=11) tAAGGCGTTTACGCCGCATCCGGCAACAGATGCCTGATGCGACGCTACCGCGTCTTATCAGGCCTACaaaa > 1:2750305/1‑68 (MQ=11) ttCACGCCGCATCCGGCAACCTATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc < 1:1398961/65‑1 (MQ=255) ttCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc > 1:4074165/1‑44 (MQ=255) ttCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc < 1:2228975/57‑1 (MQ=255) cACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGccga > 1:4244148/1‑55 (MQ=255) cACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc < 1:2803497/55‑1 (MQ=255) cACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa > 1:5346588/1‑71 (MQ=255) aCGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg > 1:3721529/1‑69 (MQ=255) cgccgcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata < 1:874482/71‑1 (MQ=255) gccgcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc > 1:1057757/1‑47 (MQ=255) ccgcATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg < 1:5328310/71‑1 (MQ=255) gcATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa > 1:5204864/1‑45 (MQ=9) gcATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa > 1:4982818/1‑45 (MQ=9) gcATCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCa > 1:3962820/1‑45 (MQ=9) cATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGGCGATGCCGat < 1:5494557/65‑1 (MQ=255) aTCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa < 1:3225822/71‑1 (MQ=255) tCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa < 1:1666800/70‑1 (MQ=255) tCCGACAACCGATGCCTGATGCGCCGCTGACGTGACTTATCAgg < 1:2415744/44‑1 (MQ=2) cGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTa > 1:2906940/1‑46 (MQ=255) cGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg < 1:1681247/71‑1 (MQ=255) cGGCAACCGATGCCTGATGCGACGCGGTCGCGTCGTATCAGGCCt > 1:765295/1‑45 (MQ=255) gCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa > 1:11565/1‑35 (MQ=255) aaCCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt < 1:4414672/71‑1 (MQ=255) aCCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTa < 1:1847385/68‑1 (MQ=255) aCCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc > 1:5370321/1‑71 (MQ=255) cGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg < 1:4107343/64‑1 (MQ=255) cGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg > 1:1782458/1‑71 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa > 1:4779939/1‑52 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa < 1:2569914/60‑1 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 1:2539130/71‑1 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 1:2651410/71‑1 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 1:448219/71‑1 (MQ=255) gATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGc < 1:4389140/71‑1 (MQ=255) aTGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTa < 1:3173683/64‑1 (MQ=255) ccTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt < 1:4894105/71‑1 (MQ=255) tGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc < 1:5147233/53‑1 (MQ=255) tGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc < 1:3036693/53‑1 (MQ=255) tGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt < 1:469343/68‑1 (MQ=255) tGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt < 1:617128/68‑1 (MQ=255) gATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc < 1:2657539/37‑1 (MQ=255) gATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTc < 1:687379/37‑1 (MQ=255) aTGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTa > 1:5690586/1‑68 (MQ=255) gCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc < 1:3684779/70‑1 (MQ=255) cGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg < 1:780192/45‑1 (MQ=255) cGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg < 1:806891/45‑1 (MQ=255) gACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg < 1:3753386/44‑1 (MQ=255) cgcgGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc > 1:2305667/1‑45 (MQ=255) cgGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTAGGCAATTAATACATAGCa > 1:5254149/1‑70 (MQ=255) ggTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCggg < 1:2633056/54‑2 (MQ=255) ggTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACg < 1:4471367/71‑1 (MQ=255) tCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt < 1:3863657/48‑1 (MQ=255) tCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt < 1:1499137/48‑1 (MQ=255) tCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt < 1:290331/48‑1 (MQ=255) tCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt < 1:5175360/48‑1 (MQ=255) tCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt < 1:318132/48‑1 (MQ=255) tCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt < 1:3295420/56‑1 (MQ=255) | TCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACG > minE/2790606‑2790744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |